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Abbreviations
#5-i. Abbreviations
- AWS EC2: Amazon Web Services, Elastic Compute
- BAM: Binary Sequence Alignment/Map
- BED: Browser Extensible Data
- CIGAR: Compact Idiosyncratic Gapped Alignment Report
- DNA: Deoxyribonucleic Acid
- FPKM: Fragments Per Kilobase Of Exon Per Million Fragments Mapped
- FPM: Fragments Per Million Fragments Mapped
- GFF: Generic Feature Format
- GTF: Gene Transfer Format
- IGB: Integrated Genome Browser
- IGV: Integrative Genome Viewer
- PCA: Principal Component Analysis
- polyA: Polyadenylation
- QC: Quality Control
- qPCR: Quantitative Polymerase Chain Reaction
- RNA: Ribonucleic acid
- RPKM: Reads Per Kilobase Of Exon Per Million Fragments Mapped
- RPM: Reads Per Million Fragments Mapped
- RT-PCR: Reverse Transcription Polymerase Chain Reaction
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##Note: The current version of this tutorial is now at www.rnaseq.wiki
Table of Contents
Module 0: Authors | Citation | Syntax | Intro to AWS | Log into AWS | Unix | Environment | Resources
Module 1: Installation | Reference Genomes | Annotations | Indexing | Data | Data QC
Module 2: Adapter Trim | Alignment | IGV | Alignment Visualization | Alignment QC
Module 3: Expression | Differential Expression | DE Visualization
Module 4: Ref Guided | De novo | Merging | Differential Splicing | Splicing Visualization
Module 5: Kallisto
Appendix: Abbreviations | Lectures | Practical Exercise Solutions | Integrated Assignment | Proposed Improvements | AWS Setup