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feat: updated version of pretext workflow developed by Delphine that takes HiFi and HiC as input. #584

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35d936d
feat: updated version of pretext workflow developed by Delphine that …
Smeds Oct 28, 2024
5ce525d
Fix connections between optional parameters and mandatory inputs,...
Delphine-L Nov 5, 2024
4e20b69
add license, authors, and diverse labels and tags
Delphine-L Nov 12, 2024
b666689
change telomere track height to correspond to the size of the telomeres
Delphine-L Nov 12, 2024
42a7240
Add README and CHANGELOG
Delphine-L Nov 12, 2024
e6c4591
update pretextgraph to fix track display issue, and add parameters to…
Delphine-L Nov 14, 2024
6cc5b23
add new parameter to the README
Delphine-L Nov 14, 2024
851f762
Remove explicit data conversion, it got fixed in galaxy
Delphine-L Nov 20, 2024
0cf305a
Rename folder
Delphine-L Nov 20, 2024
2a0c7bb
add release number and remove parameter from the READMA
Delphine-L Nov 20, 2024
4de0fbc
expose output to be used for Jbrowse2 workflow
Delphine-L Nov 20, 2024
3cf74ab
add tests
Delphine-L Nov 26, 2024
95bd01c
replace dockstore file after cleaning my local repository
Delphine-L Nov 26, 2024
2779664
add Marius comments
Delphine-L Nov 26, 2024
d4ba83e
rename folder
Delphine-L Nov 26, 2024
ce121eb
Make more clear that HiC reads need to be in collections
Delphine-L Nov 26, 2024
a8fc19f
use smaller test data
Delphine-L Nov 26, 2024
c4611ad
Remove unnecessary specification that the input is a collection
Delphine-L Nov 27, 2024
1ff2cd8
add marius comments and more details about the inputs
Delphine-L Nov 27, 2024
306265e
correct typo
Delphine-L Nov 27, 2024
4707632
use even smaller data
Delphine-L Nov 27, 2024
14c03d2
rename output of Gfastats
Delphine-L Nov 27, 2024
425d64c
update tools
Delphine-L Dec 13, 2024
db8b8ad
renaming folder with more descriptive name
Delphine-L Dec 16, 2024
67f1492
change folder name to lower case
Delphine-L Dec 16, 2024
0911819
Improve name of the Workflow
Delphine-L Dec 16, 2024
3264afa
update dockstore file
Delphine-L Dec 16, 2024
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Original file line number Diff line number Diff line change
@@ -0,0 +1,13 @@
version: 1.2
workflows:
- name: main
subclass: Galaxy
publish: true
primaryDescriptorPath: /PretextMap_Generation.ga
testParameterFiles:
- /PretextMap_Generation-tests.yml
authors:
- name: Patrik Smeds
orcid: 0000-0001-6228-2785
- name: Delphine Lariviere
orcid: 0000-0001-6421-3484
Original file line number Diff line number Diff line change
@@ -0,0 +1,5 @@
# Changelog

## [1.0] 2024-11-12

- Creation of a workflow for the generation of Hi-C Maps with coverage, gaps and Telomere Tracks
Original file line number Diff line number Diff line change
@@ -0,0 +1,87 @@
- doc: Test outline for PretextMap-Generation-from-2-haplotypes
job:
Haplotype 1:
class: File
location: https://zenodo.org/records/14230702/files/Haplotype%201.fasta
filetype: fasta
Haplotype 2:
class: File
location: https://zenodo.org/records/14230702/files/Haplotype%202.fasta
filetype: fasta
Hi-C reads - forward:
class: Collection
collection_type: list
elements:
- class: File
identifier: HiC forward reads
location: https://zenodo.org/records/14230702/files/HiC%20forward.fastqsanger.gz
Hi-C reads - reverse:
class: Collection
collection_type: list
elements:
- class: File
identifier: HI-C reverse reads
location: https://zenodo.org/records/14230702/files/HiC%20reverse.fastqsanger.gz
PacBio reads:
class: Collection
collection_type: list
elements:
- class: File
identifier: PacBio reads.fastq.gz
location: https://zenodo.org/records/14230702/files/PacBio%20reads.fastq.gz
Do you want to add suffixes to the scaffold names?: true
Haplotype 1 suffix: hap1
Haplotype 2 suffix: hap2
Do you want to trim the Hi-C data?: true
Telomere repeat to suit species: CCCTAA
outputs:
Merged Haplotypes:
asserts:
has_text:
text: ">scaffold_10.hap1"
has_text:
text: ">scaffold_10.hap2"
Gaps Bed:
asserts:
has_text:
text: "scaffold_10.hap1 34145604 34145804"
has_text:
text: "scaffold_10.hap2 137138839 137139039"
Seqtk-telo Output:
asserts:
has_text:
text: "scaffold_10.hap2 0 11012 139653677"
Gaps Bedgraph:
asserts:
has_text:
text: "scaffold_10.hap2 137138839 137139039 200"
BigWig Coverage:
asserts:
has_size:
value : 112000
delta: 4000
Telomeres Bedgraph:
asserts:
has_text:
text: "scaffold_10.hap2 0 11012 11012"
Trimmed Hi-C Forward Reads:
asserts:
has_size:
value : 13900000
delta: 2000000
Trimmed Hi-C Reverse Reads:
asserts:
has_size:
value : 14300000
delta: 2000000
Merged Hi-C Alignments:
asserts:
has_size:
value : 4600000
delta: 1000000
Pretext All tracks:
asserts:
has_size:
value : 946900
delta: 40000

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