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If VerifyBamId adds CRAM support remove dependancy on Bio::DB::Sam and switch to Bio::DB::HTS.
This will then be fully BAM/CRAM agnostic.
Modify the perl wrapper to sample the relevant locations from an input CRAM to a temporary BAM:
samtools view -F 3844 -L loci.bed -q 10 -b orig.cram | tee new.bam | samtools index - > new.bam.bai
Make the applied flags obvious in code:
4 - read unmapped (0x4) 256 - not primary alignment (0x100) 512 - read fails platform/vendor quality checks (0x200) 1024 - read is PCR or optical duplicate (0x400) 2048 - supplementary alignment (0x800)
The text was updated successfully, but these errors were encountered:
Investigate suggestion here:
statgen/verifyBamID#2
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First pass at #6
0804584
keiranmraine
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If VerifyBamId adds CRAM support remove dependancy on Bio::DB::Sam and switch to Bio::DB::HTS.
This will then be fully BAM/CRAM agnostic.
Modify the perl wrapper to sample the relevant locations from an input CRAM to a temporary BAM:
Make the applied flags obvious in code:
The text was updated successfully, but these errors were encountered: