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Update rnaseq_BLAST.md
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wyim-pgl authored Nov 20, 2023
1 parent 3e9ecc9 commit 5e835f8
Showing 1 changed file with 2 additions and 6 deletions.
8 changes: 2 additions & 6 deletions _episodes/rnaseq_BLAST.md
Original file line number Diff line number Diff line change
Expand Up @@ -513,10 +513,6 @@ cd Venn
cut -f 1 ../DEG/DESeq2.#####.dir/RSEM.isoform.counts.matrix.DT_vs_WT.DESeq2.DE_results.P0.001_C1.DT-UP.subset | grep -v sample > DESeq.UP.subset
cut -f 1 ../DEG/DESeq2.####.dir/RSEM.isoform.counts.matrix.DT_vs_WT.DESeq2.DE_results.P0.001_C1.WT-UP.subset | grep -v sample > DESeq.DOWN.subset

###edgeR
cut -f 1 ../DEG/edgeR.####.dir/RSEM.isoform.counts.matrix.DT_vs_WT.edgeR.DE_results.P0.001_C1.DT-UP.subset | grep -v sample > edgeR.UP.subset
cut -f 1 ../DEG/edgeR.####.dir/RSEM.isoform.counts.matrix.DT_vs_WT.edgeR.DE_results.P0.001_C1.WT-UP.subset | grep -v sample > edgeR.DOWN.subset

```
```bash
## IF YOU ARE NOT IN BASE ENVIRONMENT
Expand All @@ -526,8 +522,8 @@ conda activate venn
pip install --upgrade numpy pandas

### Drawing
intervene venn -i DESeq.DOWN.subset DESeq.UP.subset edgeR.DOWN.subset edgeR.UP.subset --type list --save-overlaps
intervene upset -i DESeq.DOWN.subset DESeq.UP.subset edgeR.DOWN.subset edgeR.UP.subset --type list --save-overlaps
intervene venn -i DESeq.DOWN.subset DESeq.UP.subset --type list --save-overlaps
intervene upset -i DESeq.DOWN.subset DESeq.UP.subset --type list --save-overlaps
```
### Assignment

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