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0. Set‐up
A few basic things need to be set up before we can get to the actual work. In the interests of time and speed we have setup a You'll need to create and start a VM based on a snapshot we provide that has a lot of bioinformatics software pre-installed. As you know getting bioinformatics software installed is often a time-consuming task that we have done for you.
You will need a Google account to run this workshop. If you have a google account you want to use for this workshop you can skip this step.
If you don't have a Google account you can create one at https://account.google.com by clicking on the Create account link. You'll have to go through several screens following the instructions to create the account.
Once you have an account you should be able to go to
https://console.cloud.google.com/getting-started
Go to Compute Engine->Virtual Machines -> VM Instances
Use the name "asm-ngs-$USER" with $USER replaced with your username
Set Region to "us-cental1 (Iowa)" and Zone to "us-cental1a"
In Machine Configuration, set to "e2-standard-8"
Change the standard Boot Disk by clicking on the Change button
Select Custom Images and then cbi-2024-asm-ngs-wkshp-image-10102024
Increase the size of the disk to 100 GB and click SELECT
Set allow full Access to all Cloud APIs and allow HTTPs traffic and then hit the Create button at the bottom
It will take a few minutes to create the new VM. Once it is running click the SSH button to the right of the VM list.
It may take a few moments to make the connection, transfer SSH keys, etc. You can just close the "Please consider adding..." dialog box if it appears.
If you successfully login you should be in the terminal and able to type commands in
This work was supported by the National Center for Biotechnology Information of the National Library of Medicine (NLM) and the National Institute of Allergy and Infectious disease (NIAID), National Institutes of Health