Other Input Files

Housekeeping Genes File

List of genes to use as housekeeping genes for the estimation of False-Negative probabilities

Text file containing one gene name per line If omitted, FastProject uses the default list of human housekeeping genes stored in:

<FastProject Root>/FastProject/Housekeeping Genes/

Projection File

List of X and Y coordinates for each sample

If a projection was calculated elsewhere the coordinates can be input into FastProject to be compared against signatures.

Format is tab delimited text, one sample per line. The name of the file is used when listing the projection in the output report.

Example:

Cell1	0.45	-0.24
Cell2	-0.58	1.03
Cell3	.98	0.76
...	...	...

Weights File

A weight (0.0 to 1.0) for each input measurement.

Normally weights and false-negative probabilities are estimated by FastProject. However, it is also possible to import weights estimated with another tool.

File format is text, tab-delimited. One row per gene, one column per sample/cell. Same format as the input gene expression matrix.

This file is specified by using the --weights option when running FastProject