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Do I need spliced fastq? #120

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HyojeenLee opened this issue Dec 1, 2022 · 0 comments
Open

Do I need spliced fastq? #120

HyojeenLee opened this issue Dec 1, 2022 · 0 comments

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@HyojeenLee
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HyojeenLee commented Dec 1, 2022 •
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Hello,

I am now following the tutorial for single cell, RNA seq, somatic mutation calling.
I am wondering if the input fastq file had to be spliced (spicing introns) in advance of the workflow?

Otherwise, does CTAT automatically remove intron part for analysis?

Thank you.
@HyojeenLee HyojeenLee reopened this Dec 1, 2022
@HyojeenLee HyojeenLee changed the title Can I use mmi reference and splice option together? Do I need spliced fastq? Dec 1, 2022
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