SCALE and droplet-based sequencing protocols

[JamesDanielOReilly]

Hello,

Before I spend time trying to figure out how exactly to use the tool, I wanted to ask if SCALE can be used on data generated from droplet-based sequencing, without spike-ins. Specifically, I'm using CROP-seq data. Thanks for your time.

All the best,
James

[yuchaojiang]

No you would not have enough coverage / heterozygous loci. Yuchao

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Sent from my iPhone On May 5, 2021, at 4:26 AM, JamesDanielOReilly ***@***.***> wrote:  Hello, Before I spend time trying to figure out how exactly to use the tool, I wanted to ask if SCALE can be used on data generated from droplet-based sequencing, without spike-ins. Specifically, I'm using CROP-seq data. Thanks for your time. All the best, James — You are receiving this because you are subscribed to this thread. Reply to this email directly, view it on GitHub<#11>, or unsubscribe<https://github.com/notifications/unsubscribe-auth/ACNKFSLA2E4H3RDN46X7ZWLTMD6JXANCNFSM44EGUWGQ>.