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#!/bin/bash | ||
# | ||
# Demo script for V'DJer | ||
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# Before running this script: | ||
# Run make to generate the vdjer executable. | ||
# Download the vdjer references and untar, updating the VDJER_REF_DIR variable below. | ||
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VDJER_REF_DIR=<path/to/vdjer/referenes>/igh | ||
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VDJER=../vdjer | ||
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# Run V'DJer on the input BAM file for the IgH chain. | ||
# read length = 50 | ||
# median insert size = 175 | ||
# num threads = 4 | ||
# | ||
# Assembled contigs appear in vdj_contigs.fa | ||
# Reads mapped to contigs appear in vdjer.sam | ||
$VDJER --in star.sort.bam --rl 50 --t 4 --ins 175 --chain IGH --ref-dir $VDJER_REF_DIR > vdjer.sam 2> vdjer.log |
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