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# Evaluating binning results | ||
# Evaluating binning results | ||
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Run `gbintk evaluate --help` or `gbintk evaluate -h` to list the help message for evaluation. | ||
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```shell | ||
Usage: gbintk evaluate [OPTIONS] | ||
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Evaluate the binning results given a ground truth | ||
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Options: | ||
--binned PATH path to the .csv file with the initial binning | ||
output from an existing tool [required] | ||
--groundtruth PATH path to the .csv file with the ground truth | ||
[required] | ||
--delimiter [,|;| |" "] delimiter for input/output results. Supports a | ||
comma (,), a semicolon (;), a tab ($'\t'), a space | ||
(" ") and a pipe (|) [default: ,] | ||
--output PATH path to the output folder [required] | ||
-h, --help Show this message and exit. | ||
``` |
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# Using GraphBin2 | ||
# Using GraphBin2 | ||
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Run `gbintk graphbin2 --help` or `gbintk graphbin2 -h` to list the help message for GraphBin2. | ||
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```shell | ||
Usage: gbintk graphbin2 [OPTIONS] | ||
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GraphBin2: Refined and Overlapped Binning of Metagenomic Contigs Using | ||
Assembly Graphs | ||
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Options: | ||
--assembler [spades|megahit|flye] | ||
name of the assembler used (SPAdes, MEGAHIT | ||
or Flye) [required] | ||
--graph PATH path to the assembly graph file [required] | ||
--contigs PATH path to the contigs file [required] | ||
--paths PATH path to the contigs.paths (metaSPAdes) or | ||
assembly.info (metaFlye) file | ||
--abundance PATH path to the abundance file [required] | ||
--binned PATH path to the .csv file with the initial | ||
binning output from an existing tool | ||
[required] | ||
--output PATH path to the output folder [required] | ||
--prefix TEXT prefix for the output file | ||
--depthb INTEGER maximum depth for the breadth-first-search. | ||
[default: 5] | ||
--threshold FLOAT threshold for determining inconsistent | ||
vertices. [default: 1.5] | ||
--delimiter [,|;|$'\t'|" "] delimiter for input/output results. Supports | ||
a comma (,), a semicolon (;), a tab ($'\t'), | ||
a space (" ") and a pipe (|) [default: ,] | ||
--nthreads INTEGER number of threads to use. [default: 8] | ||
-h, --help Show this message and exit. | ||
``` |
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# Using GraphBin | ||
# Using GraphBin | ||
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Run `gbintk graphbin --help` or `gbintk graphbin -h` to list the help message for GraphBin. | ||
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```shell | ||
Usage: gbintk graphbin [OPTIONS] | ||
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GraphBin: Refined Binning of Metagenomic Contigs using Assembly Graphs | ||
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Options: | ||
--assembler [spades|megahit|flye] | ||
name of the assembler used (SPAdes, MEGAHIT | ||
or Flye) [required] | ||
--graph PATH path to the assembly graph file [required] | ||
--contigs PATH path to the contigs file [required] | ||
--paths PATH path to the contigs.paths (metaSPAdes) or | ||
assembly.info (metaFlye) file | ||
--binned PATH path to the .csv file with the initial | ||
binning output from an existing tool | ||
[required] | ||
--output PATH path to the output folder [required] | ||
--prefix TEXT prefix for the output file | ||
--max_iteration INTEGER maximum number of iterations for label | ||
propagation algorithm [default: 100] | ||
--diff_threshold FLOAT RANGE difference threshold for label propagation | ||
algorithm [default: 0.1; 0<=x<=1] | ||
--delimiter [,|;|$'\t'|" "] delimiter for input/output results. Supports | ||
a comma (,), a semicolon (;), a tab ($'\t'), | ||
a space (" ") and a pipe (|) [default: ,] | ||
-h, --help Show this message and exit. | ||
``` |
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# Using MetaCoAG | ||
# Using MetaCoAG | ||
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Run `gbintk metacoag --help` or `gbintk metacoag -h` to list the help message for MetaCoAG. | ||
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```shell | ||
Usage: gbintk metacoag [OPTIONS] | ||
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MetaCoAG: Binning Metagenomic Contigs via Composition, Coverage and Assembly | ||
Graphs | ||
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Options: | ||
--assembler [spades|megahit|flye] | ||
name of the assembler used (SPAdes, MEGAHIT | ||
or Flye) [required] | ||
--graph PATH path to the assembly graph file [required] | ||
--contigs PATH path to the contigs file [required] | ||
--paths PATH path to the contigs.paths (metaSPAdes) or | ||
assembly.info (metaFlye) file | ||
--abundance PATH path to the abundance file [required] | ||
--output PATH path to the output folder [required] | ||
--hmm TEXT path to marker.hmm file. [default: | ||
auxiliary/marker.hmm] | ||
--prefix TEXT prefix for the output file | ||
--min_length INTEGER minimum length of contigs to consider for | ||
binning. [default: 1000] | ||
--p_intra FLOAT RANGE minimum probability of an edge matching to | ||
assign to the same bin. [default: 0.1; | ||
0<=x<=1] | ||
--p_inter FLOAT RANGE maximum probability of an edge matching to | ||
create a new bin. [default: 0.01; 0<=x<=1] | ||
--d_limit INTEGER distance limit for contig matching. | ||
[default: 20] | ||
--depthlp INTEGER depth to consider for label propagation. | ||
[default: 10] | ||
--n_mg INTEGER total number of marker genes. [default: | ||
108] | ||
--no_cut_tc do not use --cut_tc for hmmsearch. | ||
--mg_threshold FLOAT RANGE length threshold to consider marker genes. | ||
[default: 0.5; 0<=x<=1] | ||
--bin_mg_threshold FLOAT RANGE minimum fraction of marker genes that should | ||
be present in a bin. [default: 0.33333; | ||
0<=x<=1] | ||
--min_bin_size INTEGER minimum size of a bin to output in base | ||
pairs (bp). [default: 200000] | ||
--delimiter [,|;|$'\t'|" "] delimiter for input/output results. Supports | ||
a comma (,), a semicolon (;), a tab ($'\t'), | ||
a space (" ") and a pipe (|) [default: ,] | ||
--nthreads INTEGER number of threads to use. [default: 8] | ||
-h, --help Show this message and exit. | ||
``` |
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# Formatting binning results for GraphBin/GraphBin2 | ||
# Formatting binning results for GraphBin/GraphBin2 | ||
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Run `gbintk prepare --help` or `gbintk prepare -h` to list the help message for formatting. | ||
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```shell | ||
Usage: gbintk prepare [OPTIONS] | ||
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Format the initial binning result from an existing binning tool | ||
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Options: | ||
--assembler [spades|megahit|flye] | ||
name of the assembler used (SPAdes, MEGAHIT | ||
or Flye) [required] | ||
--resfolder PATH path to the folder containing FASTA files | ||
for individual bins [required] | ||
--delimiter [,|;| |" "] delimiter for input/output results. Supports | ||
a comma (,), a semicolon (;), a tab ($'\t'), | ||
a space (" ") and a pipe (|) [default: ,] | ||
--prefix TEXT prefix for the output file | ||
--output PATH path to the output folder [required] | ||
-h, --help Show this message and exit. | ||
``` |
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# Visualising binning results | ||
# Visualising binning results | ||
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Run `gbintk visualise --help` or `gbintk visualise -h` to list the help message for visualisation. | ||
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```shell | ||
Usage: gbintk visualise [OPTIONS] | ||
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Visualise binning and refinement results | ||
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Options: | ||
--assembler [spades|megahit|flye] | ||
name of the assembler used (SPAdes, MEGAHIT | ||
or Flye) [required] | ||
--initial PATH path to the initial binning result | ||
[required] | ||
--final PATH path to the final binning result [required] | ||
--graph PATH path to the assembly graph file [required] | ||
--paths PATH path to the contigs.paths (metaSPAdes) or | ||
assembly.info (metaFlye) file | ||
--output PATH path to the output folder [required] | ||
--prefix TEXT prefix for the output file | ||
--dpi INTEGER dpi value [default: 300] | ||
--width INTEGER width of the image in pixels [default: | ||
2000] | ||
--height INTEGER height of the image in pixels [default: | ||
2000] | ||
--vsize INTEGER size of the vertices [default: 50] | ||
--lsize INTEGER size of the vertex labels [default: 8] | ||
--margin INTEGER margin of the figure [default: 50] | ||
--type TEXT type of the image (jpg, png, eps, svg) | ||
[default: png] | ||
--delimiter [,|;|$'\t'|" "] delimiter for input/output results. Supports | ||
a comma (,), a semicolon (;), a tab ($'\t'), | ||
a space (" ") and a pipe (|) [default: ,] | ||
-h, --help Show this message and exit. | ||
``` |