Dev

tb-profiler

@@ -1,7 +1,7 @@
 #! /usr/bin/env python3
 import sys
 import pathogenprofiler as pp
-from pathogenprofiler import TempFilePrefix
+from pathogenprofiler import TempFilePrefix, TempFolder
 import pathogenprofiler.variant_calling as vc
 import argparse
 from rich_argparse import ArgumentDefaultsRichHelpFormatter
@@ -17,6 +17,7 @@ from joblib import Parallel, delayed
 from tqdm import tqdm
 import logging
 from rich.logging import RichHandler
+from copy import deepcopy
 
 import importlib
 import pkgutil
@@ -81,18 +82,28 @@ contents of the error log (%s)
 ###############################################################################
 """ % (outfile))
 
+def vcf_job(args: argparse.Namespace,sample_name: str):
+    # logging.info(f"\nExtracting variants and running pipeline for {sample_name}\n")
+    copy_of_args = deepcopy(args)
+    copy_of_args.prefix = sample_name
+    with TempFolder() as tmpfolder:
+        cmd = f"bcftools view -s {sample_name} -ac 1 {args.vcf} | bcftools +fixploidy -Oz -o {tmpfolder}/{args.vcf} && bcftools index {tmpfolder}/{args.vcf} " % vars(copy_of_args)
+        pp.run_cmd(cmd)
+        os.chdir(tmpfolder)
+        copy_of_args.files_prefix = os.path.abspath(f"{os.getcwd()}/{copy_of_args.prefix}")
+        main_profile(copy_of_args)
+        os.chdir('../')
 
 
 def multisample_vcf_run(args):
     vcf_obj = pp.Vcf(args.vcf)
-    args.original_vcf = args.vcf
+    jobs = []
+
     for sample_name in vcf_obj.samples:
-        logging.info(f"\nExtracting variants and running pipeline for {sample_name}\n")
-        args.prefix = sample_name
-        args.tmp_vcf = "%s.%s.vcf.gz" % (args.files_prefix,uuid4())
-        pp.run_cmd("bcftools view -s %(prefix)s -ac 1 %(original_vcf)s | bcftools +fixploidy -Oz -o %(tmp_vcf)s && bcftools index %(tmp_vcf)s " % vars(args))
-        args.vcf = args.tmp_vcf
-        main_profile(args)
+        jobs.append((args,sample_name))
+
+    parallel = Parallel(n_jobs=args.threads, return_as='generator')
+    [r for r in tqdm(parallel(delayed(vcf_job)(cmd[0],cmd[1]) for cmd in jobs),total=len(jobs),desc="Running jobs")]
 
 def create_output_directories(args,directories=["bam","vcf","results"]):
     if pp.nofolder(args.dir):

tbprofiler/__init__.py

@@ -8,7 +8,7 @@
 from .docx import *
 from abc import ABC, abstractmethod
 
-__version__ = "6.4.1"
+__version__ = "6.5.0"
 
 
 class ProfilePlugin:

tbprofiler/consensus.py

@@ -70,7 +70,8 @@ def get_consensus_vcf(sample: str,input_vcf: str,args: argparse.Namespace) -> st
     tmp_aln = str(uuid4())
     run_cmd(f"cat {args.conf['ref']} {consensus_file}> {tmp_aln}")
     outfile = f"{args.files_prefix}.masked.vcf"
-    run_cmd(f"faToVcf -includeNoAltN {tmp_aln} {outfile}")
-    os.remove(tmp_aln)
+    run_cmd(f"fa2vcf.py {tmp_aln} {outfile}")
+    run_cmd(f'rm {tmp_aln} {tmp_aln}.fai')
+
     return outfile
 

tbprofiler/snp_dists.py

@@ -12,6 +12,7 @@
 import argparse
 from .models import ProfileResult, LinkedSample
 from typing import List, Tuple
+from datetime import datetime
 
 def extract_variant_set(vcf_file: str) -> Tuple[set,set]:
     ref_diffs = set()
@@ -74,6 +75,7 @@ def store(self,result: ProfileResult, vcf_file: str) -> None:
         self.missing = missing
     def search(self,result: ProfileResult, vcf_file: str, cutoff: int = 20) -> List[LinkedSample]:
         logging.info("Searching for close samples in %s" % self.filename)
+        start_timestamp = datetime.now()
         self.c.execute("SELECT sample, diffs, missing FROM variants WHERE lineage=?",(result.sub_lineage,))
         self.diffs,self.missing = extract_variant_set(vcf_file)
         sample_dists = []
@@ -89,6 +91,8 @@ def search(self,result: ProfileResult, vcf_file: str, cutoff: int = 20) -> List[
                         positions = list(dist)
                     )
                 )
+        end_timestamp = datetime.now()
+        logging.info("Finished searching for close samples in %s. Took %s" % (self.filename,end_timestamp-start_timestamp))
         logging.info("Found %s close samples" % len(sample_dists))
         return sample_dists