The notebooks in this repo contain analysis of serum escape mutants to validate data for XBB.1.5 library experiments
Data in this repo includes:
- Raw infectivity data for each mutant. At: data/virus_titer_RLU/virus_titers.csv
- Fraction infectivity data for each serum neutralization. At: data/neuts/serum_neuts_batch2_frac_infectivity.csv
- DMS escape data taken from XBB.1.5 library experiments. At: data/dms
config file contains paths to files above.
Notebooks in this repo are as follows:
- Measure rescued luciferase-carrying virus infectivity. Notebook: virus_titers.ipynb.
- Plot neutralization curves for each virus mutant and compare to DMS data. Notebook: virus_neutralization.ipynb
plasmid_maps folder includes plasmid maps for each mutants used in neutralization experiments as well as plasmids used in XBB.1.5 and BA.2 library production experiments.
Outputs from each notebook can be found at results folder.
By Bernadeta Dadonaite