Skip to content

ivars-silamikelis/fastq_qual_trimmer

Folders and files

NameName
Last commit message
Last commit date

Latest commit

 

History

14 Commits
 
 
 
 
 
 
 
 
 
 
 
 

Repository files navigation

#fastq_qual_trimmer ##What it does? It trims and filters Sanger encoded FASTQ files by quality, length and homopolymer count. ##Installation To install on Unix systems with git, type:

git clone https://github.com/ivars-silamikelis/fastq_qual_trimmer
cd fastq_qual_trimmer
make

binary file will appear that can be copied to /usr/local/bin/ or added to your $PATH

##Usage If you wish to trim by quality 25 both ends of reads in FASTQ file named test.fq, type

./fastq_qual_trimmer -i test.fq -q 25 

##Available options

Option Argument Description
-h None Prints help message
-i Filename Specifies input file
-q Integer Smallest allowed quality for nucleotides at read ends (default: 20)
-m Integer Smallest allowed mean read quality (default 0)
-l Integer Minimal allowed read length (default 1)
-H Integer Reads with homopolymers with specified length or higher will be filtered. Use 0 to switch off (default: 0)
-w None Use sliding window trimming. Trim nucleotides from both ends by calculating mean quality of nucleotides in the window (default: off)
-s Integer Specify size of sliding window (default: 5)
-b None Use sliding window and after that - default trimming approaches (default: off)

About

No description, website, or topics provided.

Resources

Stars

Watchers

Forks

Releases

No releases published

Packages

No packages published