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| <code>extras\/microscope_settings\.json</code> | | **[QA/QC]** A file generated by the micro-meta app that contains a description of the settings that were used to acquire the image data. Email HuBMAP Consortium Help Desk <[email protected]> if help is required in generating this document. | | | ||
| <code>raw\/.*</code> | ✓ | This is a directory containing raw data. | | | ||
| <code>lab_processed\/images\/[^\/]+\.ome\.tiff</code> | ✓ | OME-TIFF file (multichannel, multi-layered) produced by the experiment. If compressed, must use loss-less compression algorithm. See the following link for the set of fields that are required in the OME TIFF file XML header. <https://docs.google.com/spreadsheets/d/1YnmdTAA0Z9MKN3OjR3Sca8pz-LNQll91wdQoRPSP6Q4/edit#gid=0> | | | ||
| <code>lab_processed\/images\/[^\/]*ome-tiff\.channels\.csv</code> | ✓ | This file should describe any processing that was done to generate the images in each channel of the accommpanying OME TIFF. The file should contain one row per OME TIFF channel. Two columns should be booleans "is this a channel to use for nuclei segmentation" and "is this a channel to use for cell segmentation". | | | ||
| <code>lab_processed\/images\/[^\/]*ome-tiff\.channels\.csv</code> | ✓ | This file should describe any processing that was done to generate the images in each channel of the accompanying OME TIFF. The file should contain one row per OME TIFF channel. Two columns should be booleans "is this a channel to use for nuclei segmentation" and "is this a channel to use for cell segmentation". | | | ||
| <code>lab_processed\/annotations\/.*</code> | | Directory containing segmentation masks. | | | ||
| <code>lab_processed\/annotations\/[^\/]+\.segmentations\.ome\.tiff</code> | | The segmentation masks should be stored as multi-channel pyramidal OME TIFF bitmasks with one channel per mask, where a single mask contains all instances of a type of object (e.g., all cells, a class of FTUs, etc). The class of objects contained in the mask is documented in the segmentation-masks.csv file. Each individual object in a mask should be represented by a unique integer pixel value starting at 1, with 0 meaning background (e.g., all pixels belonging to the first instance of a T-cell have a value of 1, the pixels for the second instance of a T-cell have a value of 2, etc). The pixel values should be unique within a mask. FTUs and other structural elements should be captured the same way as cells with segmentation masks and the appropriate channel feature definitions. | lab_processed\/annotations\/.* | | ||
| <code>lab_processed\/annotations\/segmentation-masks\.csv</code> | | This file contains details about each mask, with one row per mask. Each column in this file contains details describing the mask (e.g., channel number, mask name, ontological ID, etc). Each mask is stored as a channel in the segmentations.ome.tiff file and the mask name should be ontologically based and linked to the ASCT+B table where possible. The number of rows in this file should equal the number of channels in the segmentations.ome.tiff. For example, one row in this file would ontologically describe cells, if the segmentations.ome.tiff file contained a mask of all cells. A minimum set of fields (required and optional) is included below. If multiple segmentations.ome.tiff files are used, this segmentation-masks.csv file should document the masks across all of the OME TIFF files. | lab_processed\/annotations\/.* | | ||
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Moved to [github pages](https://hubmapconsortium.github.io/ingest-validation-tools/segmentation-mask/). |
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--- | ||
title: Segmentation Mask | ||
schema_name: segmentation-mask | ||
category: Derived Datasets | ||
all_versions_deprecated: False | ||
exclude_from_index: False | ||
layout: default | ||
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--- | ||
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Related files: | ||
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Excel and TSV templates for this schema will be available when the draft next-generation schema, to be used in all future submissions, is finalized (no later than Sept. 30). | ||
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For additional documentation on Segmentation Masks, please visit [here](https://docs.google.com/document/d/1TSQon8nTIoyA5bEKxd8IAKYO6nsDabGbbQ8uKN1gj4E). | ||
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## Metadata schema | ||
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<summary><a href="https://docs.google.com/spreadsheets/d/1sMMyKtrxD_PO4TVj0JhOpeLF0fRYe2Fjmxnhp-fNzdM"><b>Version 2 (use this one)</b> (draft - submission of data prepared using this schema will be supported by Sept. 30)</a></summary> | ||
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<br> | ||
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## Directory schemas | ||
<summary><a href="https://docs.google.com/spreadsheets/d/1lAGryXYfGIP0jBXjIopB7PtFfo6RqC2bw3RUI4GzTWQ"><b>Version 2 (use this one)</b> (draft - submission of data prepared using this schema will be supported by Sept. 30) </a></summary> | ||
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