Can't read the genome sequence

[zhidaobuzhidao]

Hi,
When evaluating genomes, are there any requirements for genome size or individual sequence length?
I am now evaluating a 25G genome, but the run log shows no sequence read.
As follows

[M::[email protected]1.00] read 0 bases in 0 contigs
[M::[email protected]1.00] 0 blocks
[M::[email protected]1.00] collected syncmers
[M::[email protected]1.00] 0 kmer-block pairs

How can I solve this problem?
Looking forward to reply!

[huangnengCSU]

Hi,
This should be the bug of miniprot aligning proteins to large chromosome (maybe greater than 2Gb) #59. We need to wait for miniprot to fix this problem.

A sub-optimal solution is separating the large chromosome into several parts and each part has a size less than 1.5Gb. Then evaluate the assembly with new chromosome. This may have a little impact on the evaluation result.

[zhidaobuzhidao]

Thanks for your reply and suggestions！
It is no problem to cut chromosomes into contig to run.