Skip to content

Commit

Permalink
beta 2.1
Browse files Browse the repository at this point in the history
  • Loading branch information
hsnguyen committed Aug 14, 2020
1 parent 33df30a commit 74b25f3
Showing 1 changed file with 8 additions and 2 deletions.
10 changes: 8 additions & 2 deletions docs/npgraph-ru.md
Original file line number Diff line number Diff line change
Expand Up @@ -55,12 +55,18 @@ Options:
The provided functionality is similar to *npGraph* except no consensus is supported since MSA is slow for this time-critical server. Also PAF is the only alignment format used for rRPC communications since its light-weighted property.

### How to
To use the pipeline, first you need to familiarize yourself with the experiment of [*ReadFish*](https://github.com/LooseLab/readfish). A slight modification of this repo to operate with *npGraphServer* is [*here*](https://github.com/hsnguyen/ru).
To use the pipeline, first you need to familiarize yourself with [*ReadFish*](https://github.com/LooseLab/readfish). A slight modification of this repo to operate with *npGraphServer* is [*here*](https://github.com/hsnguyen/ru). So after install original ReadUntil code, you'll have to update (from the virtualenv) by
```
pip install -U git+https://github.com/hsnguyen/ru@master
```

Beside the Guppy basecall server, you'll need to start a *npGraphServer* as well for the run.

After finishing all installation, you can start to test running selective sequencing with *npGraphServer*.Beside the Guppy basecall server, you'll need to start a *npGraphServer* as well for the run.
```
java -cp target/assembly-x.x.x.jar org.rtassembly.NPGraphServerCmd -si assembly_graph.[fastg|gfa] -output output/ -read 1 -expect 20000 -gui
```
Follow the procedure similar to *ReadFish* but using the [*npgraph_selection.toml*](https://github.com/hsnguyen/ru/blob/master/examples/npgraph_selection.toml) instead.

## Reference


Expand Down

0 comments on commit 74b25f3

Please sign in to comment.