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nextflow.config
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params {
h =null
help =null
fqlist = "fq.lst"
// a txt file that recording the fastq file list.
//Each row either have two columns as samplename and R1.fastq.gz
// or have three columns as samplename, R1.fastq.gz and R2.fastq.gz
outdir = 'result'
Trim_Mode = null
// use this parameter to determine how to trim adaptors 1-4;
species = "hsa"
//select species. set hsa for human or mmu for mouse.
prefix = "sRNAseq"
A = null //NEXTflex
}
profiles {
local {
process.executor = 'local'
process {
withName: Trim_Galore_NEXTflex {
module = ['trimgalore/0.6.6', 'fastqc/0.11.8', 'python37']
memory = '8GB'
}
}
process {
withName: Trim_Galore_Custom {
module = ['trimgalore/0.6.6', 'fastqc/0.11.8', 'python37']
memory = '8GB'
}
}
process {
withName: Cat_SingleEndFastqGz_MultiLanes {
memory = '8GB'
}
}
process {
withName: Collapse_FqToFa {
module = ['perl/5.20.1']
memory = '8GB'
}
}
process {
withName: Run_Animal {
module = ['bowtie/1.2.2','R/4.3.2','samtools/1.9','bedtools/2.25.0']
memory = '30GB'
}
}
process {
withName: Join_Tables {
module = 'R/4.3.2'
memory = '8GB'
}
}
}
cluster {
process.executor = 'lsf'
executor.queueSize = 500
process.queue = 'priority' //'standard'
process.scratch=true
process.clusterOptions = '-P sRNAtools'
executor.jobName = { "$task.name - $task.hash" }
process {
withName: Trim_Galore_NEXTflex {
module = ['trimgalore/0.6.6', 'fastqc/0.11.8', 'python37']
memory = '8GB'
}
}
process {
withName: Trim_Galore_Custom {
module = ['trimgalore/0.6.6', 'fastqc/0.11.8', 'python37']
memory = '8GB'
}
}
process {
withName: Cat_SingleEndFastqGz_MultiLanes {
memory = '8GB'
}
}
process {
withName: Collapse_FqToFa {
module = ['perl/5.20.1']
memory = '8GB'
}
}
process {
withName: Run_Animal {
module = ['bowtie/1.2.2','R/4.3.2','samtools/1.9','bedtools/2.25.0']
memory = '30GB'
}
}
process {
withName: Join_Tables {
module = 'R/4.3.2'
memory = '8GB'
}
}
}
}