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New Filter, Plot, and Explore Tutorial Using Seurat Tools! #4759

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Apr 9, 2024
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9990bfa
First Half (Through QC Plots) of Seurat Buttons
Camila-goclowski Feb 28, 2024
e2d1590
changed tutorial title
Camila-goclowski Feb 28, 2024
08243b9
added through choosing thresholds
Camila-goclowski Feb 28, 2024
89aad91
Through filtering cells
Camila-goclowski Feb 28, 2024
c51ccc1
fixed other tutorial references
Camila-goclowski Feb 28, 2024
c2a34c9
Through processing
Camila-goclowski Feb 29, 2024
57ff669
Through Dim Reduc and UMAP Visualization
Camila-goclowski Feb 29, 2024
a16679d
Through first look plotting
Camila-goclowski Feb 29, 2024
d684b7d
through DE
Camila-goclowski Feb 29, 2024
c64143e
beginings of tech. assessment
Camila-goclowski Feb 29, 2024
ce6525f
added plotting markers for cell type ID
Camila-goclowski Feb 29, 2024
528e6bb
hid dev tool boxes for rendering
Camila-goclowski Feb 29, 2024
01bafc7
Merge branch 'galaxyproject:main' into FPE_SeuratTools
Camila-goclowski Feb 29, 2024
92beb70
small edit
Camila-goclowski Feb 29, 2024
3e482ae
full deleted developer tools to hopefully render
Camila-goclowski Feb 29, 2024
fb72108
fixed tip box
Camila-goclowski Feb 29, 2024
c097067
Merge branch 'galaxyproject:main' into FPE_SeuratTools
Camila-goclowski Mar 12, 2024
0920a43
Updated available tools (removed links to missing ones)
Camila-goclowski Mar 21, 2024
33e6076
fixed broken images
Camila-goclowski Mar 21, 2024
f5ce50d
images fix
Camila-goclowski Mar 21, 2024
c5c9f6c
reformatting numbered sections
Camila-goclowski Mar 21, 2024
388bb4b
Merge branch 'galaxyproject:main' into FPE_SeuratTools
Camila-goclowski Mar 21, 2024
adffea1
Merge branch 'galaxyproject:main' into FPE_SeuratTools
Camila-goclowski Mar 25, 2024
a5eb2a3
updated plot and umap tools!
Camila-goclowski Mar 25, 2024
eb033d5
Heading Level fixes
Camila-goclowski Mar 25, 2024
cf3ee39
Removed redundant default values from tool params
Camila-goclowski Mar 25, 2024
4878968
small fixes through processing
Camila-goclowski Mar 25, 2024
7a85141
Added Pablo as an author
Camila-goclowski Mar 26, 2024
7db3e54
Update topics/single-cell/tutorials/scrna-case_FilterPlotandExplore_S…
Camila-goclowski Apr 2, 2024
0be5509
Update topics/single-cell/tutorials/scrna-case_FilterPlotandExplore_S…
Camila-goclowski Apr 2, 2024
dcf7287
Update topics/single-cell/tutorials/scrna-case_FilterPlotandExplore_S…
Camila-goclowski Apr 2, 2024
9933db3
Update topics/single-cell/tutorials/scrna-case_FilterPlotandExplore_S…
Camila-goclowski Apr 2, 2024
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Update topics/single-cell/tutorials/scrna-case_FilterPlotandExplore_S…
Camila-goclowski Apr 2, 2024
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Update topics/single-cell/tutorials/scrna-case_FilterPlotandExplore_S…
Camila-goclowski Apr 2, 2024
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Camila-goclowski Apr 2, 2024
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Update topics/single-cell/tutorials/scrna-case_FilterPlotandExplore_S…
Camila-goclowski Apr 2, 2024
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Update topics/single-cell/tutorials/scrna-case_FilterPlotandExplore_S…
Camila-goclowski Apr 2, 2024
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Update topics/single-cell/tutorials/scrna-case_FilterPlotandExplore_S…
Camila-goclowski Apr 2, 2024
c906e58
Update topics/single-cell/tutorials/scrna-case_FilterPlotandExplore_S…
Camila-goclowski Apr 2, 2024
ff4f3e7
Update topics/single-cell/tutorials/scrna-case_FilterPlotandExplore_S…
Camila-goclowski Apr 2, 2024
af931b4
Update topics/single-cell/tutorials/scrna-case_FilterPlotandExplore_S…
Camila-goclowski Apr 3, 2024
6028d45
Update topics/single-cell/tutorials/scrna-case_FilterPlotandExplore_S…
Camila-goclowski Apr 3, 2024
5a0ee28
Update topics/single-cell/tutorials/scrna-case_FilterPlotandExplore_S…
Camila-goclowski Apr 3, 2024
416e274
Update topics/single-cell/tutorials/scrna-case_FilterPlotandExplore_S…
Camila-goclowski Apr 3, 2024
73c3277
Update topics/single-cell/tutorials/scrna-case_FilterPlotandExplore_S…
Camila-goclowski Apr 3, 2024
a4b16bc
Update topics/single-cell/tutorials/scrna-case_FilterPlotandExplore_S…
Camila-goclowski Apr 3, 2024
4718fc3
Update topics/single-cell/tutorials/scrna-case_FilterPlotandExplore_S…
Camila-goclowski Apr 3, 2024
1a9712c
Update topics/single-cell/tutorials/scrna-case_FilterPlotandExplore_S…
Camila-goclowski Apr 3, 2024
27170f6
Update topics/single-cell/tutorials/scrna-case_FilterPlotandExplore_S…
Camila-goclowski Apr 3, 2024
8a28462
Update topics/single-cell/tutorials/scrna-case_FilterPlotandExplore_S…
Camila-goclowski Apr 3, 2024
6d416a7
Update topics/single-cell/tutorials/scrna-case_FilterPlotandExplore_S…
Camila-goclowski Apr 3, 2024
661224f
Update topics/single-cell/tutorials/scrna-case_FilterPlotandExplore_S…
Camila-goclowski Apr 3, 2024
34bf3ff
Update topics/single-cell/tutorials/scrna-case_FilterPlotandExplore_S…
Camila-goclowski Apr 3, 2024
f4a6bfe
Update topics/single-cell/tutorials/scrna-case_FilterPlotandExplore_S…
Camila-goclowski Apr 3, 2024
4474037
Update topics/single-cell/tutorials/scrna-case_FilterPlotandExplore_S…
Camila-goclowski Apr 3, 2024
35bddd0
Update topics/single-cell/tutorials/scrna-case_FilterPlotandExplore_S…
Camila-goclowski Apr 3, 2024
c2dff43
Update topics/single-cell/tutorials/scrna-case_FilterPlotandExplore_S…
Camila-goclowski Apr 3, 2024
3c15a56
Update topics/single-cell/tutorials/scrna-case_FilterPlotandExplore_S…
Camila-goclowski Apr 3, 2024
0e80ef7
Fixed featurws vs feature and RDS file input formatting
Camila-goclowski Apr 3, 2024
10f7653
changed suggested tutorials
Camila-goclowski Apr 3, 2024
b6dd9b5
suggested --> requirements
Camila-goclowski Apr 3, 2024
a6e335f
Added Elbow Plot
Camila-goclowski Apr 3, 2024
ff3951b
Fixed Plot errors @ end
Camila-goclowski Apr 3, 2024
8146a35
Fixed remaining plots
Camila-goclowski Apr 3, 2024
93a773b
removed double PNG tags on images
Camila-goclowski Apr 3, 2024
531118d
Made alt text of figures more descriptive -- thank you Helena for poi…
Camila-goclowski Apr 3, 2024
96e5f0c
Renamed Objects to make easier to follow along
Camila-goclowski Apr 3, 2024
6de40e0
added choose your own tutorial
Camila-goclowski Apr 4, 2024
ba4d694
'' instead of ""
Camila-goclowski Apr 4, 2024
48e6c53
[hopefully] corrected choose your own tutorial
Camila-goclowski Apr 4, 2024
0547574
removed icon tool
Camila-goclowski Apr 4, 2024
a10edc4
Merge branch 'galaxyproject:main' into FPE_SeuratTools
Camila-goclowski Apr 4, 2024
72a306e
Must use "safe" titles, consistent formatting
hexylena Apr 5, 2024
d8eb83b
It cannot be a tip: it is a mandatory step
hexylena Apr 5, 2024
39321ab
combine get data and generate seurat objects into one section
pavanvidem Apr 5, 2024
21241d5
add get data under Zenodo option
pavanvidem Apr 5, 2024
b845b46
Update topics/single-cell/tutorials/scrna-case_FilterPlotandExplore_S…
pavanvidem Apr 5, 2024
4181a5f
fix the remaininf class names
pavanvidem Apr 5, 2024
be5929b
switch back to spaces in the options
pavanvidem Apr 5, 2024
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Original file line number Diff line number Diff line change
@@ -0,0 +1,78 @@
# This is the bibliography file for your tutorial.
#
# To add bibliography (bibtex) entries here, follow these steps:
# 1) Find the DOI for the article you want to cite
# 2) Go to https://doi2bib.org and fill in the DOI
# 3) Copy the resulting bibtex entry into this file
#
# To cite the example below, in your tutorial.md file
# use {% cite Batut2018 %}
#
# If you want to cite an online resourse (website etc)
# you can use the 'online' format (see below)
#
# You can remove the examples below

@article{Bacon2018,
doi = {10.3389/fimmu.2018.02523},
url = {https://doi.org/10.3389/fimmu.2018.02523},
year = {2018},
month = nov,
publisher = {Frontiers Media {SA}},
volume = {9},
author = {Wendi A. Bacon and Russell S. Hamilton and Ziyi Yu and Jens Kieckbusch and Delia Hawkes and Ada M. Krzak and Chris Abell and Francesco Colucci and D. Stephen Charnock-Jones},
title = {Single-Cell Analysis Identifies Thymic Maturation Delay in Growth-Restricted Neonatal Mice},
journal = {Frontiers in Immunology}
}

@article{Wolf2018,
doi = {10.1186/s13059-017-1382-0},
url = {https://doi.org/10.1186/s13059-017-1382-0},
year = {2018},
month = feb,
publisher = {Springer Science and Business Media {LLC}},
volume = {19},
number = {1},
author = {F. Alexander Wolf and Philipp Angerer and Fabian J. Theis},
title = {{SCANPY}: large-scale single-cell gene expression data analysis},
journal = {Genome Biology}
}

@article{Satija2015,
doi = {10.1038/nbt.3192},
url = {https://doi.org/10.1038/nbt.3192},
year = {2015},
month = May,
publisher = {Nature Anerica Inc. All rights reserved},
volume = {33},
number = {5},
author = {Rahul Satija and Jeffrey A. Farrell and David Gennert and Alexander F Schier and Aviv Regev},
title = {Spatial reconstruction of single-cell gene expression data},
journal = {Nature Biotechnology}
}

@article{Trapnell2014,
doi = {10.1038/nbt.2859},
url = {https://doi.org/10.1038/nbt.2859},
year = {2014},
month = April,
publisher = {Nature Anerica Inc. All rights reserved},
volume = {32},
number = {4},
author = {Cole Trapnell and Davide Cacchiarelli and Jonna Grimsby and Prapti Pokharel and Shuqiang Li and Michael Morse and Niall J Lennon and Kenneth J Livak and Tarjei S Mikkelsen and John L Rinn},
title = {Pseudo-temporal ordering of individual cells reveals dynamics and regulators of cell fate decisions},
journal = {Nature Biotechnology}
}

@article{McCarthy2017,
doi = {10.1093/bioinformatics/btw777},
url = {https://doi.org/10.1093/bioinformatics/btw777},
year = {2017},
month = January,
publisher = {Advanced Access Publication},
volume = {33},
number = {8},
author = {Davis J McCarthy and Kieran R Campbell and Aaron T L Lun and Quin F Wills},
title = {Scater: pre-processing, quality control, normalization and visualization of single-cell RNA-seq data in R},
journal = {Bioinformatics}
}

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