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clean tools parameters and input files
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yvanlebras authored Dec 13, 2024
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Showing 1 changed file with 7 additions and 8 deletions.
15 changes: 7 additions & 8 deletions topics/ecology/tutorials/ENA_Biodiv_submission/tutorial.md
Original file line number Diff line number Diff line change
Expand Up @@ -73,7 +73,7 @@ The goal is to present an accessible and reproductible workflow for data submiss
>
> {% snippet faqs/galaxy/datasets_change_datatype.md %}
>
> 5. **Build a Collection** containing these two files
> 5. **Build a Collection** containing these two files, you can ame i "ab1" for example
>
> {% snippet faqs/galaxy/collections_build_list.md %}
>
Expand All @@ -88,10 +88,7 @@ Following steps take as input ab1 sequences files and produce filtered FastQ and
> <hands-on-title> ab1 to FASTQ converter </hands-on-title>
> 1. {% tool [ab1 to FASTQ converter](toolshed.g2.bx.psu.edu/repos/ecology/ab1_fastq_converter/ab1_fastq_converter/1.20.0) %} with the following parameters:
> - {% icon param-file %} *"Input ab1 file"*: `A2_RC_8F2_B.pl_HCOI.ab1`
> 2. Repeat this step for the file `A12_RC_9G4_B.md_HCOI.ab1`
>
>
> - {% icon param-file %} *"Input ab1 file"*: `ab1` data collection created at the previous step
>
{: .hands_on}
Expand All @@ -101,12 +98,14 @@ Following steps take as input ab1 sequences files and produce filtered FastQ and
We are doing a first Quality control on the raw files using FastQC and MultiQC.
> <hands-on-title> FastQC </hands-on-title>
> 1. {% tool [FastQC](toolshed.g2.bx.psu.edu/repos/devteam/fastqc/fastqc/0.74+galaxy0) %} on the Fastq files:
>
> 2. {% tool [MultiQC](toolshed.g2.bx.psu.edu/repos/iuc/multiqc/multiqc/1.11+galaxy1) %} with the following parameters:
> 1. {% tool [FastQC](toolshed.g2.bx.psu.edu/repos/devteam/fastqc/fastqc/0.74+galaxy0) %} with the following parameters:
> - {% icon param-file %} *"Raw read data from your current history"*: `ab1.fastq` data collection created at the previous step
> 2. {% tool [MultiQC](toolshed.g2.bx.psu.edu/repos/iuc/multiqc/multiqc/1.11+galaxy1) %} with the following parameters:
> - In *"Results"*:
> - {% icon param-repeat %} *"Insert Results"*
> - *"Which tool was used generate logs?"*: `FastQC`
> - In *"FastQC output"*:
> - {% icon param-file %} *"RawData FastQC output"*: `FastQC on collection X:` data collection created at the previous step
>
> 3. *Check on the HTML files the general quality statistics of your sequences*
>
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