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Add the option "untrimmed reads" to the first splitting step
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This avoids having an empty fastq file in the output collection that causes problems with alignments .
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Delphine-L authored Mar 31, 2022
1 parent a133660 commit 026b43f
Showing 1 changed file with 13 additions and 12 deletions.
25 changes: 13 additions & 12 deletions topics/genome-annotation/tutorials/tnseq/tutorial.md
Original file line number Diff line number Diff line change
Expand Up @@ -196,9 +196,10 @@ We would like now to split our Tnseq reads in `Tnseq-Tutorial-reads` given the b
> - In *"Adapter Options"*
> - *"Maximum error rate"*: `0.15` (to allow 1 mismatch)
> - *"Match times"*: `3` (to cover cases where barcodes are attached several times)
> - In *"Output Options"*
> - *"Report"*: `Yes`
> - *"Multiple output"*: `Yes` (to separate the reads into one file per condition)
> - In *"Output Selector"*, select
> - *"Report"*
> - *"Multiple output"* (to separate the reads into one file per condition)
> - *"Untrimmed reads"* (to write reads that do not contain the adapter to a separate file)
>
> The output is a collection of the different conditions datasets, here control and condition, and a report text file.
>
Expand Down Expand Up @@ -237,8 +238,8 @@ Our reads are now divided by condition. We need to trim their tail containing th
> - *"Enter custom 3' adapter sequence"*: `CGTTATGGCACGC`
> - In *"Adapter Options"*
> - *"Match times"*: `3` (to cover cases where barcodes are attached several times)
> - In *"Output Options"*
> - *"Report"*: `Yes`
> - In *"Output Selector"*, select
> - *"Report"*
>
> > ### {% icon question %} Questions
> > What are the outputs at this step?
Expand All @@ -262,8 +263,8 @@ Our reads are now divided by condition. We need to trim their tail containing th
> - In *"Filter Options"*
> - *"Minimum length"* : `64`
> - *"Maximum length"* : `70`
> - In *"Output Options"*
> - *"Report"*: `Yes`
> - In *"Output Selector"*, select
> - *"Report"*
>
> 4. Inspect the generated report files
>
Expand Down Expand Up @@ -312,9 +313,9 @@ The constructs used in this experiment contain different strengths and direction
> - {% icon param-file %} *"Choose file containing 5' adapters"*: `construct_barcodes` file
> - In *"Adapter Options"*
> - *"Match times"*: `3` (to cover cases where barcodes are attached several times)
> - In *"Output Options"*
> - *"Report"*: `Yes`
> - *"Multiple output"* : `Yes` (to separate the reads into one file per condition)
> - In *"Output Selector"*, select
> - *"Report"*
> - *"Multiple output"* (to separate the reads into one file per condition)
>
> 2. Inspect the report files
>
Expand Down Expand Up @@ -347,8 +348,8 @@ The last remaining transposon sequence in the reads is the linker with the MmeI
> - *"Enter custom 3' adapter sequence"*: `ACAGGTTGGATGATAAGTCCCCGGTCTATATTGAGAGTAACTACATTT`
> - In *"Adapter Options"*
> - *"Maximum error rate"*: `0.15`
> - In *"Output Options"*
> - *"Report"*: `Yes`
> - In *"Output Selector"*, select
> - *"Report"*
>
> 2. Inspect the report files and check that the majority of the read have been trimmed
{: .hands_on}
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