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RSeQC has some useful modules but is super super slow (looking at you geneBody_coverage.py). Some we have in there currently are probably also not so useful and are clogging up the MultiQC report.
I started working on this on branch refactor_units_qc, but we should come to a decision as to what to include/remove. Annoyingly all of these tools require different format annotation files (GTFs/BED12/refFlat etc.) so need to do some interconversion.
RSeQC has some useful modules but is super super slow (looking at you
geneBody_coverage.py
). Some we have in there currently are probably also not so useful and are clogging up the MultiQC report.RNA-SeQC - https://github.com/getzlab/rnaseqc - has a lot of the same functionality but is much faster. Mainly would like to use this for strandedness reporting & gene body coverage profiles but there should be other useful metrics in there. https://github.com/getzlab/rnaseqc/blob/master/Metrics.md
I also liked the look of Picard's collectRNASeqMetrics - https://broadinstitute.github.io/picard/picard-metric-definitions.html#RnaSeqMetrics - but have to decide how much of this is already covered by other tools and whether this is necessary.
I started working on this on branch
refactor_units_qc
, but we should come to a decision as to what to include/remove. Annoyingly all of these tools require different format annotation files (GTFs/BED12/refFlat etc.) so need to do some interconversion.Some other possible additions - https://github.com/zavolanlab/tin-score-calculation - handy to know if seeing extensive RNA degradation within samples. We'd have to plug this into multiQC ourselves, though this has already been done in their pipeline https://github.com/zavolanlab/zarp
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