Skip to content
New issue

Have a question about this project? Sign up for a free GitHub account to open an issue and contact its maintainers and the community.

By clicking “Sign up for GitHub”, you agree to our terms of service and privacy statement. We’ll occasionally send you account related emails.

Already on GitHub? Sign in to your account

Refine minimal QC modules and reporting #38

Open
SamBryce-Smith opened this issue Apr 19, 2022 · 0 comments
Open

Refine minimal QC modules and reporting #38

SamBryce-Smith opened this issue Apr 19, 2022 · 0 comments
Assignees

Comments

@SamBryce-Smith
Copy link
Member

SamBryce-Smith commented Apr 19, 2022

RSeQC has some useful modules but is super super slow (looking at you geneBody_coverage.py). Some we have in there currently are probably also not so useful and are clogging up the MultiQC report.

RNA-SeQC - https://github.com/getzlab/rnaseqc - has a lot of the same functionality but is much faster. Mainly would like to use this for strandedness reporting & gene body coverage profiles but there should be other useful metrics in there. https://github.com/getzlab/rnaseqc/blob/master/Metrics.md

I also liked the look of Picard's collectRNASeqMetrics - https://broadinstitute.github.io/picard/picard-metric-definitions.html#RnaSeqMetrics - but have to decide how much of this is already covered by other tools and whether this is necessary.

I started working on this on branch refactor_units_qc, but we should come to a decision as to what to include/remove. Annoyingly all of these tools require different format annotation files (GTFs/BED12/refFlat etc.) so need to do some interconversion.

Some other possible additions - https://github.com/zavolanlab/tin-score-calculation - handy to know if seeing extensive RNA degradation within samples. We'd have to plug this into multiQC ourselves, though this has already been done in their pipeline https://github.com/zavolanlab/zarp

Sign up for free to join this conversation on GitHub. Already have an account? Sign in to comment
Labels
None yet
Projects
None yet
Development

No branches or pull requests

1 participant