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v0.2.11 #18

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Mar 26, 2022
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2 changes: 1 addition & 1 deletion README.md
Original file line number Diff line number Diff line change
Expand Up @@ -38,7 +38,7 @@ To process data locally using SEQC, you must install the <a href=https://github.
Once all dependencies have been installed, SEQC can be installed by running:

```bash
export SEQC_VERSION="0.2.10"
export SEQC_VERSION="0.2.11"
wget https://github.com/hisplan/seqc/archive/v${SEQC_VERSION}.tar.gz
tar xvzf v${SEQC_VERSION}.tar.gz
cd seqc-${SEQC_VERSION}
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8 changes: 4 additions & 4 deletions docs/README.md
Original file line number Diff line number Diff line change
Expand Up @@ -8,9 +8,9 @@

## Generating Reference Packages

This generates a reference package (STAR index and GTF) using SEQC v0.2.10.
This generates a reference package (STAR index and GTF) using SEQC.

- Ensembl 86
- Ensembl 85
- Gene annotation file that contains only the reference chromosomes (no scaffolds, no patches)
- Only these biotypes: 'protein_coding', 'lincRNA', 'IG_V_gene', 'IG_C_gene', 'IG_J_gene', 'TR_C_gene', 'TR_J_gene', 'TR_V_gene', 'TR_D_gene', 'IG_D_gene'
- Not passing anything to `--additional-id-types`
Expand All @@ -22,7 +22,7 @@ This generates a reference package (STAR index and GTF) using SEQC v0.2.10.
SEQC index \
-o homo_sapiens \
-f homo_sapiens \
--ensemble-release 93 \
--ensemble-release 85 \
--valid-biotypes protein_coding lincRNA antisense IG_V_gene IG_D_gene IG_J_gene IG_C_gene TR_V_gene TR_D_gene TR_J_gene TR_C_gene \
--read-length 101 \
--folder ./test-data/index/ \
Expand All @@ -35,7 +35,7 @@ SEQC index \
SEQC index \
-o homo_sapiens \
-f homo_sapiens \
--ensemble-release 93 \
--ensemble-release 85 \
--valid-biotypes protein_coding lincRNA antisense IG_V_gene IG_D_gene IG_J_gene IG_C_gene TR_V_gene TR_D_gene TR_J_gene TR_C_gene \
--read-length 101 \
--upload-prefix s3://dp-lab-test/seqc/index/86/ \
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6 changes: 3 additions & 3 deletions docs/install-SUSE.md
Original file line number Diff line number Diff line change
Expand Up @@ -43,8 +43,8 @@ conda install -c bioconda star
## Install SEQC

```
wget https://github.com/dpeerlab/seqc/archive/v0.2.10.tar.gz
tar xvzf v0.2.10.tar.gz
cd seqc-0.2.10/
wget https://github.com/dpeerlab/seqc/archive/v0.2.11.tar.gz
tar xvzf v0.2.11.tar.gz
cd seqc-0.2.11/
pip install .
```
3 changes: 3 additions & 0 deletions src/seqc/core/run.py
Original file line number Diff line number Diff line change
Expand Up @@ -533,6 +533,9 @@ def create_read_array(
args.output_prefix + "_sparse_molecule_counts.mtx",
args.output_prefix + "_sparse_counts_barcodes.csv",
args.output_prefix + "_sparse_counts_genes.csv",
"raw_feature_bc_matrix/matrix.mtx.gz",
"raw_feature_bc_matrix/barcodes.tsv.gz",
"raw_feature_bc_matrix/features.tsv.gz",
]

if os.path.exists(args.output_prefix + "_cb-correction.csv.gz"):
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2 changes: 1 addition & 1 deletion src/seqc/io.py
Original file line number Diff line number Diff line change
Expand Up @@ -136,7 +136,7 @@ def download_awscli(cls, link, prefix='./', overwrite=True, recursive=False):
'provided link %s was a prefix but download was not called recursively. '
'Please provide a filename or download recursively.' % link)

cmd = 'aws s3 cp %s %s' % (link, prefix)
cmd = 'aws s3 cp --force-glacier-transfer %s %s' % (link, prefix)
if recursive:
cmd += ' --recursive'

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2 changes: 1 addition & 1 deletion src/seqc/version.py
Original file line number Diff line number Diff line change
@@ -1 +1 @@
__version__ = "0.2.10"
__version__ = "0.2.11"