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Merge pull request #8 from nf-core/dev
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Dev
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d4straub authored Jul 14, 2020
2 parents b08d336 + b377a82 commit 44b6ea1
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22 changes: 18 additions & 4 deletions docs/output.md
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Expand Up @@ -166,13 +166,27 @@ Sequencing depth per contig and sample is generated by `jgi_summarize_bam_contig

- `<assembler>-<assembly>-depth.txt.gz`: Sequencing depth for each contig and sample, only for short reads.

### Metabat
### MetaBAT2

[metabat](https://bitbucket.org/berkeleylab/metabat) recovers genome bins (that is, contigs/scaffolds that all belongs to a same organism) from metagenome assemblies. Additionally, Quast is run again on all the genome bins.
[MetaBAT2](https://bitbucket.org/berkeleylab/metabat) recovers genome bins (that is, contigs/scaffolds that all belongs to a same organism) from metagenome assemblies.

**output directory: `results/GenomeBinning/MetaBat2`**
**output directory: `results/GenomeBinning/MetaBAT2`**

- `*.fa`: Genome bins retrieved from the different input assemblies
- `$assembler-$sample.*.fa`: Genome bins retrieved from input assembly
- `$assembler-$sample.unbinned.*.fa`: Contigs that were not binned with other contigs but considered interesting. By default, these are at least 1 Mbp (`--min_length_unbinned_contigs`) in length and at most the 100 longest contigs (`--max_unbinned_contigs`) are reported.

All the files in this folder will be assessed by Quast and Busco.

**output directory: `results/GenomeBinning/MetaBAT2/discarded`**

- `*.lowDepth.fa`: Low depth contigs that are filtered by MetaBat2
- `*.tooShort.fa`: Too short contigs that are filtered by MetaBat2
- `*.unbinned.pooled.fa`: Pooled unbinned contigs equal or above `--min_contig_size`, by default 1500 bp.
- `*.unbinned.remaining.fa`: Remaining unbinned contigs below `--min_contig_size`, by default 1500 bp, but not in any other file.

All the files in this folder contain small and/or unbinned contigs that are not further processed.

Files in these two folders contain all contigs of an assembly.

### Busco

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