diff --git a/404.html b/404.html
index ef75f12..eb7cda1 100644
--- a/404.html
+++ b/404.html
@@ -318,8 +318,8 @@ <h1>Page not found</h1>
   "url": "https://ccb-hms.github.io/osca-carpentries/404.html",
   "identifier": "https://ccb-hms.github.io/osca-carpentries/404.html",
   "dateCreated": "2024-01-10",
-  "dateModified": "2024-04-02",
-  "datePublished": "2024-04-02"
+  "dateModified": "2024-04-09",
+  "datePublished": "2024-04-09"
 }
 
   </script><script>
diff --git a/CODE_OF_CONDUCT.html b/CODE_OF_CONDUCT.html
index 89ad579..6a026b1 100644
--- a/CODE_OF_CONDUCT.html
+++ b/CODE_OF_CONDUCT.html
@@ -256,7 +256,7 @@ <h2 class="accordion-header" id="flush-headingTwelve">
       </div>
       <hr></nav><main id="main-content" class="main-content"><div class="container lesson-content">
         <h1>Contributor Code of Conduct</h1>
-        <p>Last updated on 2024-03-25 |
+        <p>Last updated on 2024-04-09 |
         
         <a href="https://github.com/ccb-hms/osca-carpentries/edit/main/CODE_OF_CONDUCT.md" class="external-link">Edit this page <i aria-hidden="true" data-feather="edit"></i></a></p>
         
@@ -339,8 +339,8 @@ <h1>Contributor Code of Conduct</h1>
   "url": "https://ccb-hms.github.io/osca-carpentries/CODE_OF_CONDUCT.html",
   "identifier": "https://ccb-hms.github.io/osca-carpentries/CODE_OF_CONDUCT.html",
   "dateCreated": "2024-01-10",
-  "dateModified": "2024-03-25",
-  "datePublished": "2024-04-02"
+  "dateModified": "2024-04-09",
+  "datePublished": "2024-04-09"
 }
 
   </script><script>
diff --git a/LICENSE.html b/LICENSE.html
index 1a34a2b..51e0d76 100644
--- a/LICENSE.html
+++ b/LICENSE.html
@@ -256,7 +256,7 @@ <h2 class="accordion-header" id="flush-headingTwelve">
       </div>
       <hr></nav><main id="main-content" class="main-content"><div class="container lesson-content">
         <h1>Licenses</h1>
-        <p>Last updated on 2024-03-25 |
+        <p>Last updated on 2024-04-09 |
         
         <a href="https://github.com/ccb-hms/osca-carpentries/edit/main/LICENSE.md" class="external-link">Edit this page <i aria-hidden="true" data-feather="edit"></i></a></p>
         
@@ -390,8 +390,8 @@ <h1>Licenses</h1>
   "url": "https://ccb-hms.github.io/osca-carpentries/LICENSE.html",
   "identifier": "https://ccb-hms.github.io/osca-carpentries/LICENSE.html",
   "dateCreated": "2024-01-10",
-  "dateModified": "2024-03-25",
-  "datePublished": "2024-04-02"
+  "dateModified": "2024-04-09",
+  "datePublished": "2024-04-09"
 }
 
   </script><script>
diff --git a/aio.html b/aio.html
index 17ed3d2..11f9bef 100644
--- a/aio.html
+++ b/aio.html
@@ -306,7 +306,7 @@ <h2 class="accordion-header" id="flush-headingTwelve">
         
 <section id="aio-intro-sce"><p>Content from <a href="intro-sce.html">Introduction to Bioconductor and the SingleCellExperiment class</a></p>
 <hr>
-<p>Last updated on 2024-03-25 |
+<p>Last updated on 2024-04-09 |
         
         <a href="https://github.com/ccb-hms/osca-carpentries/edit/main/episodes/intro-sce.Rmd" class="external-link">Edit this page <i aria-hidden="true" data-feather="edit"></i></a></p>
 <div class="text-end">
@@ -778,7 +778,7 @@ <h3 class="callout-title">Exercise 1<a class="anchor" aria-label="anchor" href="
 <button class="accordion-button hint-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseHint1" aria-expanded="false" aria-controls="collapseHint1">
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 </button>
-<div id="collapseHint1" class="accordion-collapse collapse" data-bs-parent="#accordionHint1" aria-labelledby="headingHint1">
+<div id="collapseHint1" class="accordion-collapse collapse" aria-labelledby="headingHint1" data-bs-parent="#accordionHint1">
 <div class="accordion-body">
 <p>The <code>SingleCellExperiment</code> function can be used to create
 a new SingleCellExperiment object</p>
@@ -791,7 +791,7 @@ <h4 class="accordion-header" id="headingHint1">Give me a hint</h4>
 <button class="accordion-button solution-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseSolution1" aria-expanded="false" aria-controls="collapseSolution1">
   <h4 class="accordion-header" id="headingSolution1">Show me the solution</h4>
 </button>
-<div id="collapseSolution1" class="accordion-collapse collapse" data-bs-parent="#accordionSolution1" aria-labelledby="headingSolution1">
+<div id="collapseSolution1" class="accordion-collapse collapse" aria-labelledby="headingSolution1" data-bs-parent="#accordionSolution1">
 <div class="accordion-body">
 <p>TODO</p>
 </div>
@@ -819,7 +819,7 @@ <h3 class="callout-title">Exercise 2<a class="anchor" aria-label="anchor" href="
 <button class="accordion-button solution-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseSolution2" aria-expanded="false" aria-controls="collapseSolution2">
   <h4 class="accordion-header" id="headingSolution2">Show me the solution</h4>
 </button>
-<div id="collapseSolution2" class="accordion-collapse collapse" data-bs-parent="#accordionSolution2" aria-labelledby="headingSolution2">
+<div id="collapseSolution2" class="accordion-collapse collapse" aria-labelledby="headingSolution2" data-bs-parent="#accordionSolution2">
 <div class="accordion-body">
 <p>TODO</p>
 </div>
@@ -862,7 +862,7 @@ <h1 id="further-reading">Further Reading<a class="anchor" aria-label="anchor" hr
  -->
 </div></section><section id="aio-eda_qc"><p>Content from <a href="eda_qc.html">Exploratory data analysis and quality control</a></p>
 <hr>
-<p>Last updated on 2024-03-25 |
+<p>Last updated on 2024-04-09 |
         
         <a href="https://github.com/ccb-hms/osca-carpentries/edit/main/episodes/eda_qc.Rmd" class="external-link">Edit this page <i aria-hidden="true" data-feather="edit"></i></a></p>
 <div class="text-end">
@@ -2027,7 +2027,7 @@ <h3 class="callout-title">Exercise 1: Normalization<a class="anchor" aria-label=
 <button class="accordion-button solution-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseSolution1" aria-expanded="false" aria-controls="collapseSolution1">
   <h4 class="accordion-header" id="headingSolution1">Show me the solution</h4>
 </button>
-<div id="collapseSolution1" class="accordion-collapse collapse" data-bs-parent="#accordionSolution1" aria-labelledby="headingSolution1">
+<div id="collapseSolution1" class="accordion-collapse collapse" aria-labelledby="headingSolution1" data-bs-parent="#accordionSolution1">
 <div class="accordion-body">
 <p>TODO</p>
 </div>
@@ -2055,7 +2055,7 @@ <h3 class="callout-title">Exercise 2: Dimensionality Reduction<a class="anchor"
 <button class="accordion-button hint-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseHint1" aria-expanded="false" aria-controls="collapseHint1">
   <h4 class="accordion-header" id="headingHint1">Give me a hint</h4>
 </button>
-<div id="collapseHint1" class="accordion-collapse collapse" data-bs-parent="#accordionHint1" aria-labelledby="headingHint1">
+<div id="collapseHint1" class="accordion-collapse collapse" aria-labelledby="headingHint1" data-bs-parent="#accordionHint1">
 <div class="accordion-body">
 <p>First subset the object to include only highly variable genes
 (<code>sce2 &lt;- sce[hvg.sce.var,]</code>) and then apply the
@@ -2070,7 +2070,7 @@ <h4 class="accordion-header" id="headingHint1">Give me a hint</h4>
 <button class="accordion-button solution-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseSolution2" aria-expanded="false" aria-controls="collapseSolution2">
   <h4 class="accordion-header" id="headingSolution2">Show me the solution</h4>
 </button>
-<div id="collapseSolution2" class="accordion-collapse collapse" data-bs-parent="#accordionSolution2" aria-labelledby="headingSolution2">
+<div id="collapseSolution2" class="accordion-collapse collapse" aria-labelledby="headingSolution2" data-bs-parent="#accordionSolution2">
 <div class="accordion-body">
 <p>TODO</p>
 </div>
@@ -2097,7 +2097,7 @@ <h3 class="callout-title">Exercise 3: PBMC Data<a class="anchor" aria-label="anc
 <button class="accordion-button solution-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseSolution3" aria-expanded="false" aria-controls="collapseSolution3">
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 </button>
-<div id="collapseSolution3" class="accordion-collapse collapse" data-bs-parent="#accordionSolution3" aria-labelledby="headingSolution3">
+<div id="collapseSolution3" class="accordion-collapse collapse" aria-labelledby="headingSolution3" data-bs-parent="#accordionSolution3">
 <div class="accordion-body">
 <p>TODO</p>
 </div>
@@ -2128,7 +2128,7 @@ <h1 id="references">References<a class="anchor" aria-label="anchor" href="#refer
  -->
 </div></section><section id="aio-cell_type_annotation"><p>Content from <a href="cell_type_annotation.html">Cell type annotation</a></p>
 <hr>
-<p>Last updated on 2024-03-25 |
+<p>Last updated on 2024-04-09 |
         
         <a href="https://github.com/ccb-hms/osca-carpentries/edit/main/episodes/cell_type_annotation.Rmd" class="external-link">Edit this page <i aria-hidden="true" data-feather="edit"></i></a></p>
 <div class="text-end">
@@ -3343,7 +3343,7 @@ <h3 class="callout-title">Key Points<a class="anchor" aria-label="anchor" href="
  -->
 </div></section><section id="aio-multi-sample"><p>Content from <a href="multi-sample.html">Multi-sample analyses</a></p>
 <hr>
-<p>Last updated on 2024-03-25 |
+<p>Last updated on 2024-04-09 |
         
         <a href="https://github.com/ccb-hms/osca-carpentries/edit/main/episodes/multi-sample.Rmd" class="external-link">Edit this page <i aria-hidden="true" data-feather="edit"></i></a></p>
 <div class="text-end">
@@ -4413,7 +4413,7 @@ <h3 class="callout-title">Key Points<a class="anchor" aria-label="anchor" href="
  -->
 </div></section><section id="aio-large_data"><p>Content from <a href="large_data.html">Working with large data</a></p>
 <hr>
-<p>Last updated on 2024-03-25 |
+<p>Last updated on 2024-04-09 |
         
         <a href="https://github.com/ccb-hms/osca-carpentries/edit/main/episodes/large_data.Rmd" class="external-link">Edit this page <i aria-hidden="true" data-feather="edit"></i></a></p>
 <div class="text-end">
@@ -4879,24 +4879,20 @@ <h3 class="code-label">OUTPUT<i aria-hidden="true" data-feather="chevron-left"><
 <pre class="output" tabindex="0"><code>     approx
 exact   1   2   3   4   5   6   7   8   9  10  11  12  13  14  15
    1   90   0   0   0   0   0   0   0   1   0   0   0   0   0   0
-   2    0 143   0   1   0   0   0   0   0   0   0   0   0   0   0
+   2    0 143   0   0   0   0   0   0   0   0   0   0   0   0   1
    3    0   0  75   0   0   0   0   0   0   0   0   0   0   0   0
-   4    0   0   0 253   0   0   0   0   0   0   0   0 144   0   0
-   5    0   0   0   0  71   0   0  95   0   0   0   5   0   0   0
-   6    0   0   0   0   0 210   0   0   0   0   0   0   0   0   0
-   7    0   0   0   0   0   1 244   0   0   1   0   0   0   0   0
-   8    1   0   0   0   1   0   0   0 106   0   0   0   0   0   0
-   9    0   0   0   0   0   0   0   0   0 112   0  10   0   0   0
-   10   0   0   0   0   0   1   0   0   0   1 145   0   0   6   0
-   11   0   0   0   0   3   0   0   0   0   0   0 197   0   0   0
-   12   0   0   2   0 324   0   0   0   0   1   0   0   0   0   0
-   13   0   0   0   0   0   0   0   0   0   1   0   0   0 146   0
-   14   0   0   0   0   0   0   0   0   0   0   0   0   0   0  20</code></pre>
-</div>
-<div class="codewrapper">
-<h3 class="code-label">ERROR<i aria-hidden="true" data-feather="chevron-left"></i><i aria-hidden="true" data-feather="chevron-right"></i>
-</h3>
-<pre class="error" tabindex="0"><code>Error: rand &gt; 0.85 is not TRUE</code></pre>
+   4    0   0   0 341   0   0   0   0   0   0   0   0   0   0   0
+   5    1   0   0   0 392   0   0   0   1   1   0   3   0   0   0
+   6    0   0   0   0   0 209   0   0   0   0   0   0   0   0   0
+   7    0   0   0   0   0   0 245   0   0   1   0   0   0   0   0
+   8    0   0   0   0   1   0   0  95   0   0   0   0   0   0   0
+   9    1   0   0   0   1   0   0   0 106   0   0   0   0   0   0
+   10   0   0   0   0   0   0   0   0   0 112   4   0   1   0   0
+   11   0   0   0   0   0   1   0   0   0   0 143   0   0   0   0
+   12   0   0   0   0   3   0   0   0   0  10   0 201   0   0   0
+   13   0   0   0   0   0   0   0   0   0   0   6   0 146   0   0
+   14   0   0   0   0   0   0   0   0   0   0   0   0   0  20   0
+   15   0   0   0   0   0   0   0   0   0   0   0   0   0   0  56</code></pre>
 </div>
 <p>Note that Annoy writes the NN index to disk prior to performing the
 search. Thus, it may not actually be faster than the default exact
@@ -4905,7 +4901,7 @@ <h3 class="code-label">ERROR<i aria-hidden="true" data-feather="chevron-left"></
 also not difficult to find situations where the approximation
 deteriorates, especially at high dimensions, though this may not have an
 appreciable impact on the biological conclusions.</p>
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 </h3>
 <pre class="downlit sourceCode r">
@@ -4940,7 +4936,7 @@ <h2 id="singular-value-decomposition">Singular value decomposition<a class="anch
 change the SVD algorithm used in any of these functions by simply
 specifying an alternative value for the <code>BSPARAM</code>
 argument.</p>
-<div class="codewrapper sourceCode" id="cb32">
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 </h3>
 <pre class="downlit sourceCode r">
@@ -4966,7 +4962,7 @@ <h3 class="code-label">OUTPUT<i aria-hidden="true" data-feather="chevron-left"><
   .. ..$ : chr [1:500] "ENSMUSG00000055609" "ENSMUSG00000052217" "ENSMUSG00000069919" "ENSMUSG00000048583" ...
   .. ..$ : chr [1:20] "PC1" "PC2" "PC3" "PC4" ...</code></pre>
 </div>
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@@ -5015,7 +5011,7 @@ <h2 id="seurat">Seurat<a class="anchor" aria-label="anchor" href="#seurat"></a>
 types of single-cell data. Seurat is developed and maintained by the <a href="https://satijalab.org/seurat/authors.html" class="external-link">Satija lab</a> and is
 released under the <a href="https://opensource.org/license/mit/" class="external-link">MIT
 license</a>.</p>
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@@ -5037,7 +5033,7 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 with functionality from OSCA/Bioconductor. We therefore need to first
 install the <a href="https://github.com/satijalab/seurat-data" class="external-link">SeuratData</a> package,
 which is available from GitHub only.</p>
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@@ -5046,7 +5042,7 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 </div>
 <p>We then proceed by loading all required packages and installing the
 PBMC dataset:</p>
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@@ -5055,7 +5051,7 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 </div>
 <p>We then load the dataset as an <code>SeuratObject</code> and convert
 it to a <code>SingleCellExperiment</code>.</p>
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 <pre class="downlit sourceCode r">
@@ -5069,7 +5065,7 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 <p>Seurat also allows conversion from <code>SingleCellExperiment</code>
 objects to Seurat objects; we demonstrate this here on the wild-type
 chimera mouse gastrulation dataset.</p>
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@@ -5080,7 +5076,7 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 </div>
 <p>After some processing of the dataset, the actual conversion is
 carried out with the <code>as.Seurat</code> function.</p>
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 </h3>
 <pre class="downlit sourceCode r">
@@ -5113,7 +5109,7 @@ <h2 id="scanpy">Scanpy<a class="anchor" aria-label="anchor" href="#scanpy"></a>
 to enable users and developers to easily move data between these
 frameworks to construct a multi-language analysis pipeline across
 R/Bioconductor and Python.</p>
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 </h3>
 <pre class="downlit sourceCode r">
@@ -5123,7 +5119,7 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 <code>SingleCellExperiment</code> from an H5AD file. Here, we use an
 example H5AD file contained in the <em><a href="https://bioconductor.org/packages/3.18/zellkonverter" class="external-link">zellkonverter</a></em>
 package.</p>
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 </h3>
 <pre class="downlit sourceCode r">
@@ -5171,7 +5167,7 @@ <h3 class="code-label">OUTPUT<i aria-hidden="true" data-feather="chevron-left"><
 <p>We can also write a <code>SingleCellExperiment</code> to an H5AD file
 with the <code>writeH5AD()</code> function. This is demonstrated below
 on the wild-type chimera mouse gastrulation dataset.</p>
-<div class="codewrapper sourceCode" id="cb49">
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 </h3>
 <pre class="downlit sourceCode r">
@@ -5192,7 +5188,7 @@ <h3 class="code-label">OUTPUT<i aria-hidden="true" data-feather="chevron-left"><
 <div class="section level1">
 <h1 id="session-info">Session Info<a class="anchor" aria-label="anchor" href="#session-info"></a>
 </h1>
-<div class="codewrapper sourceCode" id="cb51">
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 <pre class="downlit sourceCode r">
@@ -5355,7 +5351,7 @@ <h3 class="callout-title">Exercise 1: Out of memory representation<a class="anch
 <button class="accordion-button hint-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseHint1" aria-expanded="false" aria-controls="collapseHint1">
   <h4 class="accordion-header" id="headingHint1">Give me a hint</h4>
 </button>
-<div id="collapseHint1" class="accordion-collapse collapse" aria-labelledby="headingHint1" data-bs-parent="#accordionHint1">
+<div id="collapseHint1" class="accordion-collapse collapse" data-bs-parent="#accordionHint1" aria-labelledby="headingHint1">
 <div class="accordion-body">
 <p>See the <code>HDF5Array</code> function for reading from HDF5 and the
 <code>writeHDF5Array</code> function for writing to HDF5 from the <em><a href="https://bioconductor.org/packages/3.18/HDF5Array" class="external-link">HDF5Array</a></em>
@@ -5369,7 +5365,7 @@ <h4 class="accordion-header" id="headingHint1">Give me a hint</h4>
 <button class="accordion-button solution-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseSolution1" aria-expanded="false" aria-controls="collapseSolution1">
   <h4 class="accordion-header" id="headingSolution1">Show me the solution</h4>
 </button>
-<div id="collapseSolution1" class="accordion-collapse collapse" aria-labelledby="headingSolution1" data-bs-parent="#accordionSolution1">
+<div id="collapseSolution1" class="accordion-collapse collapse" data-bs-parent="#accordionSolution1" aria-labelledby="headingSolution1">
 <div class="accordion-body">
 <p>TODO</p>
 </div>
@@ -5397,7 +5393,7 @@ <h3 class="callout-title">Exercise 2: Parallelization<a class="anchor" aria-labe
 <button class="accordion-button hint-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseHint2" aria-expanded="false" aria-controls="collapseHint2">
   <h4 class="accordion-header" id="headingHint2">Give me a hint</h4>
 </button>
-<div id="collapseHint2" class="accordion-collapse collapse" aria-labelledby="headingHint2" data-bs-parent="#accordionHint2">
+<div id="collapseHint2" class="accordion-collapse collapse" data-bs-parent="#accordionHint2" aria-labelledby="headingHint2">
 <div class="accordion-body">
 <p>Use the function <code>system.time</code> to obtain the runtime of
 each job.</p>
@@ -5410,7 +5406,7 @@ <h4 class="accordion-header" id="headingHint2">Give me a hint</h4>
 <button class="accordion-button solution-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseSolution2" aria-expanded="false" aria-controls="collapseSolution2">
   <h4 class="accordion-header" id="headingSolution2">Show me the solution</h4>
 </button>
-<div id="collapseSolution2" class="accordion-collapse collapse" aria-labelledby="headingSolution2" data-bs-parent="#accordionSolution2">
+<div id="collapseSolution2" class="accordion-collapse collapse" data-bs-parent="#accordionSolution2" aria-labelledby="headingSolution2">
 <div class="accordion-body">
 <p>TODO</p>
 </div>
@@ -5440,7 +5436,7 @@ <h3 class="callout-title">Exercise 3: Conversion to Seurat<a class="anchor" aria
 <button class="accordion-button hint-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseHint3" aria-expanded="false" aria-controls="collapseHint3">
   <h4 class="accordion-header" id="headingHint3">Give me a hint</h4>
 </button>
-<div id="collapseHint3" class="accordion-collapse collapse" aria-labelledby="headingHint3" data-bs-parent="#accordionHint3">
+<div id="collapseHint3" class="accordion-collapse collapse" data-bs-parent="#accordionHint3" aria-labelledby="headingHint3">
 <div class="accordion-body">
 <p>Use Seurat’s <code>DimPlot</code> function.</p>
 </div>
@@ -5452,7 +5448,7 @@ <h4 class="accordion-header" id="headingHint3">Give me a hint</h4>
 <button class="accordion-button solution-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseSolution3" aria-expanded="false" aria-controls="collapseSolution3">
   <h4 class="accordion-header" id="headingSolution3">Show me the solution</h4>
 </button>
-<div id="collapseSolution3" class="accordion-collapse collapse" aria-labelledby="headingSolution3" data-bs-parent="#accordionSolution3">
+<div id="collapseSolution3" class="accordion-collapse collapse" data-bs-parent="#accordionSolution3" aria-labelledby="headingSolution3">
 <div class="accordion-body">
 <p>Use Seurat’s <code>DimPlot</code> function.</p>
 </div>
@@ -5483,7 +5479,7 @@ <h1 id="references">References<a class="anchor" aria-label="anchor" href="#refer
  -->
 </div></section><section id="aio-hca"><p>Content from <a href="hca.html">Accessing data from the Human Cell Atlas (HCA)</a></p>
 <hr>
-<p>Last updated on 2024-03-25 |
+<p>Last updated on 2024-04-09 |
         
         <a href="https://github.com/ccb-hms/osca-carpentries/edit/main/episodes/hca.Rmd" class="external-link">Edit this page <i aria-hidden="true" data-feather="edit"></i></a></p>
 <div class="text-end">
@@ -5675,7 +5671,7 @@ <h3 class="code-label">OUTPUT<i aria-hidden="true" data-feather="chevron-left"><
 </h3>
 <pre class="output" tabindex="0"><code>Rows: ??
 Columns: 10
-Database: DuckDB v0.10.0 [unknown@Linux 6.5.0-1016-azure:R 4.3.3/:memory:]
+Database: DuckDB v0.10.1 [unknown@Linux 6.5.0-1017-azure:R 4.3.3/:memory:]
 $ cell_                             &lt;chr&gt; "TTATGCTAGGGTGTTG_12", "GCTTGAACATGG…
 $ sample_                           &lt;chr&gt; "039c558ca1c43dc74c563b58fe0d6289", …
 $ cell_type                         &lt;chr&gt; "mature NK T cell", "mature NK T cel…
@@ -5738,7 +5734,7 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 <h3 class="code-label">OUTPUT<i aria-hidden="true" data-feather="chevron-left"></i><i aria-hidden="true" data-feather="chevron-right"></i>
 </h3>
 <pre class="output" tabindex="0"><code># Source:   SQL [?? x 2]
-# Database: DuckDB v0.10.0 [unknown@Linux 6.5.0-1016-azure:R 4.3.3/:memory:]
+# Database: DuckDB v0.10.1 [unknown@Linux 6.5.0-1017-azure:R 4.3.3/:memory:]
    tissue                          n
    &lt;chr&gt;                       &lt;dbl&gt;
  1 renal medulla                   6
@@ -5790,19 +5786,19 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 <h3 class="code-label">OUTPUT<i aria-hidden="true" data-feather="chevron-left"></i><i aria-hidden="true" data-feather="chevron-right"></i>
 </h3>
 <pre class="output" tabindex="0"><code># Source:   SQL [?? x 2]
-# Database: DuckDB v0.10.0 [unknown@Linux 6.5.0-1016-azure:R 4.3.3/:memory:]
-   assay                              n
-   &lt;chr&gt;                          &lt;dbl&gt;
- 1 10x 3' v3                         21
- 2 Slide-seq                          4
- 3 10x 3' v2                         27
- 4 Visium Spatial Gene Expression     7
- 5 10x 5' v1                          7
- 6 scRNA-seq                          4
- 7 Seq-Well                           2
- 8 10x 5' v2                          2
- 9 10x 3' v1                          1
-10 Smart-seq2                         1
+# Database: DuckDB v0.10.1 [unknown@Linux 6.5.0-1017-azure:R 4.3.3/:memory:]
+   assay           n
+   &lt;chr&gt;       &lt;dbl&gt;
+ 1 10x 5' v2       2
+ 2 sci-RNA-seq     1
+ 3 10x 3' v1       1
+ 4 Smart-seq2      1
+ 5 10x 3' v3      21
+ 6 Slide-seq       4
+ 7 scRNA-seq       4
+ 8 Seq-Well        2
+ 9 10x 3' v2      27
+10 10x 5' v1       7
 # ℹ more rows</code></pre>
 </div>
 </div>
@@ -5821,7 +5817,7 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 <h3 class="code-label">OUTPUT<i aria-hidden="true" data-feather="chevron-left"></i><i aria-hidden="true" data-feather="chevron-right"></i>
 </h3>
 <pre class="output" tabindex="0"><code># Source:   SQL [1 x 2]
-# Database: DuckDB v0.10.0 [unknown@Linux 6.5.0-1016-azure:R 4.3.3/:memory:]
+# Database: DuckDB v0.10.1 [unknown@Linux 6.5.0-1017-azure:R 4.3.3/:memory:]
   organism         n
   &lt;chr&gt;        &lt;dbl&gt;
 1 Homo sapiens    63</code></pre>
@@ -6111,7 +6107,7 @@ <h3 class="callout-title">Exercise 1<a class="anchor" aria-label="anchor" href="
 <button class="accordion-button solution-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseSolution1" aria-expanded="false" aria-controls="collapseSolution1">
   <h4 class="accordion-header" id="headingSolution1">Show me the solution</h4>
 </button>
-<div id="collapseSolution1" class="accordion-collapse collapse" aria-labelledby="headingSolution1" data-bs-parent="#accordionSolution1">
+<div id="collapseSolution1" class="accordion-collapse collapse" data-bs-parent="#accordionSolution1" aria-labelledby="headingSolution1">
 <div class="accordion-body">
 <div class="codewrapper sourceCode" id="cb49">
 <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i aria-hidden="true" data-feather="chevron-right"></i>
@@ -6145,7 +6141,7 @@ <h3 class="callout-title">Exercise 2<a class="anchor" aria-label="anchor" href="
 <button class="accordion-button solution-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseSolution2" aria-expanded="false" aria-controls="collapseSolution2">
   <h4 class="accordion-header" id="headingSolution2">Show me the solution</h4>
 </button>
-<div id="collapseSolution2" class="accordion-collapse collapse" aria-labelledby="headingSolution2" data-bs-parent="#accordionSolution2">
+<div id="collapseSolution2" class="accordion-collapse collapse" data-bs-parent="#accordionSolution2" aria-labelledby="headingSolution2">
 <div class="accordion-body">
 <div class="codewrapper sourceCode" id="cb50">
 <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i aria-hidden="true" data-feather="chevron-right"></i>
@@ -6179,7 +6175,7 @@ <h3 class="callout-title">Exercise 3<a class="anchor" aria-label="anchor" href="
 <button class="accordion-button solution-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseSolution3" aria-expanded="false" aria-controls="collapseSolution3">
   <h4 class="accordion-header" id="headingSolution3">Show me the solution</h4>
 </button>
-<div id="collapseSolution3" class="accordion-collapse collapse" aria-labelledby="headingSolution3" data-bs-parent="#accordionSolution3">
+<div id="collapseSolution3" class="accordion-collapse collapse" data-bs-parent="#accordionSolution3" aria-labelledby="headingSolution3">
 <div class="accordion-body">
 <div class="codewrapper sourceCode" id="cb51">
 <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i aria-hidden="true" data-feather="chevron-right"></i>
@@ -6221,7 +6217,7 @@ <h3 class="callout-title">Exercise 4<a class="anchor" aria-label="anchor" href="
 <button class="accordion-button solution-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseSolution4" aria-expanded="false" aria-controls="collapseSolution4">
   <h4 class="accordion-header" id="headingSolution4">Show me the solution</h4>
 </button>
-<div id="collapseSolution4" class="accordion-collapse collapse" aria-labelledby="headingSolution4" data-bs-parent="#accordionSolution4">
+<div id="collapseSolution4" class="accordion-collapse collapse" data-bs-parent="#accordionSolution4" aria-labelledby="headingSolution4">
 <div class="accordion-body">
 <div class="codewrapper sourceCode" id="cb52">
 <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i aria-hidden="true" data-feather="chevron-right"></i>
@@ -6368,8 +6364,8 @@ <h1 id="acknowledgements">Acknowledgements<a class="anchor" aria-label="anchor"
   "url": "https://ccb-hms.github.io/osca-carpentries/aio.html",
   "identifier": "https://ccb-hms.github.io/osca-carpentries/aio.html",
   "dateCreated": "2024-01-10",
-  "dateModified": "2024-04-02",
-  "datePublished": "2024-04-02"
+  "dateModified": "2024-04-09",
+  "datePublished": "2024-04-09"
 }
 
   </script><script>
diff --git a/cell_type_annotation.html b/cell_type_annotation.html
index fa483bc..218c13c 100644
--- a/cell_type_annotation.html
+++ b/cell_type_annotation.html
@@ -260,7 +260,7 @@ <h2 class="accordion-header" id="flush-headingTwelve">
       </div>
       <hr></nav><main id="main-content" class="main-content"><div class="container lesson-content">
         <h1>Cell type annotation</h1>
-        <p>Last updated on 2024-03-25 |
+        <p>Last updated on 2024-04-09 |
         
         <a href="https://github.com/ccb-hms/osca-carpentries/edit/main/episodes/cell_type_annotation.Rmd" class="external-link">Edit this page <i aria-hidden="true" data-feather="edit"></i></a></p>
         
@@ -1519,8 +1519,8 @@ <h3 class="callout-title">Key Points<a class="anchor" aria-label="anchor" href="
   "url": "https://ccb-hms.github.io/osca-carpentries/cell_type_annotation.html",
   "identifier": "https://ccb-hms.github.io/osca-carpentries/cell_type_annotation.html",
   "dateCreated": "2024-01-10",
-  "dateModified": "2024-03-25",
-  "datePublished": "2024-04-02"
+  "dateModified": "2024-04-09",
+  "datePublished": "2024-04-09"
 }
 
   </script><script>
diff --git a/eda_qc.html b/eda_qc.html
index 57f046a..44d07ab 100644
--- a/eda_qc.html
+++ b/eda_qc.html
@@ -260,7 +260,7 @@ <h2 class="accordion-header" id="flush-headingTwelve">
       </div>
       <hr></nav><main id="main-content" class="main-content"><div class="container lesson-content">
         <h1>Exploratory data analysis and quality control</h1>
-        <p>Last updated on 2024-03-25 |
+        <p>Last updated on 2024-04-09 |
         
         <a href="https://github.com/ccb-hms/osca-carpentries/edit/main/episodes/eda_qc.Rmd" class="external-link">Edit this page <i aria-hidden="true" data-feather="edit"></i></a></p>
         
@@ -1397,7 +1397,7 @@ <h3 class="callout-title">Exercise 1: Normalization<a class="anchor" aria-label=
 <button class="accordion-button solution-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseSolution1" aria-expanded="false" aria-controls="collapseSolution1">
   <h4 class="accordion-header" id="headingSolution1">Show me the solution</h4>
 </button>
-<div id="collapseSolution1" class="accordion-collapse collapse" data-bs-parent="#accordionSolution1" aria-labelledby="headingSolution1">
+<div id="collapseSolution1" class="accordion-collapse collapse" aria-labelledby="headingSolution1" data-bs-parent="#accordionSolution1">
 <div class="accordion-body">
 <p>TODO</p>
 </div>
@@ -1425,7 +1425,7 @@ <h3 class="callout-title">Exercise 2: Dimensionality Reduction<a class="anchor"
 <button class="accordion-button hint-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseHint1" aria-expanded="false" aria-controls="collapseHint1">
   <h4 class="accordion-header" id="headingHint1">Give me a hint</h4>
 </button>
-<div id="collapseHint1" class="accordion-collapse collapse" data-bs-parent="#accordionHint1" aria-labelledby="headingHint1">
+<div id="collapseHint1" class="accordion-collapse collapse" aria-labelledby="headingHint1" data-bs-parent="#accordionHint1">
 <div class="accordion-body">
 <p>First subset the object to include only highly variable genes
 (<code>sce2 &lt;- sce[hvg.sce.var,]</code>) and then apply the
@@ -1440,7 +1440,7 @@ <h4 class="accordion-header" id="headingHint1">Give me a hint</h4>
 <button class="accordion-button solution-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseSolution2" aria-expanded="false" aria-controls="collapseSolution2">
   <h4 class="accordion-header" id="headingSolution2">Show me the solution</h4>
 </button>
-<div id="collapseSolution2" class="accordion-collapse collapse" data-bs-parent="#accordionSolution2" aria-labelledby="headingSolution2">
+<div id="collapseSolution2" class="accordion-collapse collapse" aria-labelledby="headingSolution2" data-bs-parent="#accordionSolution2">
 <div class="accordion-body">
 <p>TODO</p>
 </div>
@@ -1467,7 +1467,7 @@ <h3 class="callout-title">Exercise 3: PBMC Data<a class="anchor" aria-label="anc
 <button class="accordion-button solution-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseSolution3" aria-expanded="false" aria-controls="collapseSolution3">
   <h4 class="accordion-header" id="headingSolution3">Show me the solution</h4>
 </button>
-<div id="collapseSolution3" class="accordion-collapse collapse" data-bs-parent="#accordionSolution3" aria-labelledby="headingSolution3">
+<div id="collapseSolution3" class="accordion-collapse collapse" aria-labelledby="headingSolution3" data-bs-parent="#accordionSolution3">
 <div class="accordion-body">
 <p>TODO</p>
 </div>
@@ -1555,8 +1555,8 @@ <h1 id="references">References<a class="anchor" aria-label="anchor" href="#refer
   "url": "https://ccb-hms.github.io/osca-carpentries/eda_qc.html",
   "identifier": "https://ccb-hms.github.io/osca-carpentries/eda_qc.html",
   "dateCreated": "2024-01-10",
-  "dateModified": "2024-03-25",
-  "datePublished": "2024-04-02"
+  "dateModified": "2024-04-09",
+  "datePublished": "2024-04-09"
 }
 
   </script><script>
diff --git a/hca.html b/hca.html
index 351b5df..3c051a8 100644
--- a/hca.html
+++ b/hca.html
@@ -260,7 +260,7 @@ <h2 class="accordion-header" id="flush-headingTwelve">
       </div>
       <hr></nav><main id="main-content" class="main-content"><div class="container lesson-content">
         <h1>Accessing data from the Human Cell Atlas (HCA)</h1>
-        <p>Last updated on 2024-03-25 |
+        <p>Last updated on 2024-04-09 |
         
         <a href="https://github.com/ccb-hms/osca-carpentries/edit/main/episodes/hca.Rmd" class="external-link">Edit this page <i aria-hidden="true" data-feather="edit"></i></a></p>
         
@@ -429,7 +429,7 @@ <h3 class="code-label">OUTPUT<i aria-hidden="true" data-feather="chevron-left"><
 </h3>
 <pre class="output" tabindex="0"><code>Rows: ??
 Columns: 10
-Database: DuckDB v0.10.0 [unknown@Linux 6.5.0-1016-azure:R 4.3.3/:memory:]
+Database: DuckDB v0.10.1 [unknown@Linux 6.5.0-1017-azure:R 4.3.3/:memory:]
 $ cell_                             &lt;chr&gt; "TTATGCTAGGGTGTTG_12", "GCTTGAACATGG…
 $ sample_                           &lt;chr&gt; "039c558ca1c43dc74c563b58fe0d6289", …
 $ cell_type                         &lt;chr&gt; "mature NK T cell", "mature NK T cel…
@@ -490,7 +490,7 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 <h3 class="code-label">OUTPUT<i aria-hidden="true" data-feather="chevron-left"></i><i aria-hidden="true" data-feather="chevron-right"></i>
 </h3>
 <pre class="output" tabindex="0"><code># Source:   SQL [?? x 2]
-# Database: DuckDB v0.10.0 [unknown@Linux 6.5.0-1016-azure:R 4.3.3/:memory:]
+# Database: DuckDB v0.10.1 [unknown@Linux 6.5.0-1017-azure:R 4.3.3/:memory:]
    tissue                          n
    &lt;chr&gt;                       &lt;dbl&gt;
  1 renal medulla                   6
@@ -540,19 +540,19 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 <h3 class="code-label">OUTPUT<i aria-hidden="true" data-feather="chevron-left"></i><i aria-hidden="true" data-feather="chevron-right"></i>
 </h3>
 <pre class="output" tabindex="0"><code># Source:   SQL [?? x 2]
-# Database: DuckDB v0.10.0 [unknown@Linux 6.5.0-1016-azure:R 4.3.3/:memory:]
-   assay                              n
-   &lt;chr&gt;                          &lt;dbl&gt;
- 1 10x 3' v3                         21
- 2 Slide-seq                          4
- 3 10x 3' v2                         27
- 4 Visium Spatial Gene Expression     7
- 5 10x 5' v1                          7
- 6 scRNA-seq                          4
- 7 Seq-Well                           2
- 8 10x 5' v2                          2
- 9 10x 3' v1                          1
-10 Smart-seq2                         1
+# Database: DuckDB v0.10.1 [unknown@Linux 6.5.0-1017-azure:R 4.3.3/:memory:]
+   assay           n
+   &lt;chr&gt;       &lt;dbl&gt;
+ 1 10x 5' v2       2
+ 2 sci-RNA-seq     1
+ 3 10x 3' v1       1
+ 4 Smart-seq2      1
+ 5 10x 3' v3      21
+ 6 Slide-seq       4
+ 7 scRNA-seq       4
+ 8 Seq-Well        2
+ 9 10x 3' v2      27
+10 10x 5' v1       7
 # ℹ more rows</code></pre>
 </div>
 </div>
@@ -570,7 +570,7 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 <h3 class="code-label">OUTPUT<i aria-hidden="true" data-feather="chevron-left"></i><i aria-hidden="true" data-feather="chevron-right"></i>
 </h3>
 <pre class="output" tabindex="0"><code># Source:   SQL [1 x 2]
-# Database: DuckDB v0.10.0 [unknown@Linux 6.5.0-1016-azure:R 4.3.3/:memory:]
+# Database: DuckDB v0.10.1 [unknown@Linux 6.5.0-1017-azure:R 4.3.3/:memory:]
   organism         n
   &lt;chr&gt;        &lt;dbl&gt;
 1 Homo sapiens    63</code></pre>
@@ -852,7 +852,7 @@ <h3 class="callout-title">Exercise 1<a class="anchor" aria-label="anchor" href="
 <button class="accordion-button solution-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseSolution1" aria-expanded="false" aria-controls="collapseSolution1">
   <h4 class="accordion-header" id="headingSolution1">Show me the solution</h4>
 </button>
-<div id="collapseSolution1" class="accordion-collapse collapse" aria-labelledby="headingSolution1" data-bs-parent="#accordionSolution1">
+<div id="collapseSolution1" class="accordion-collapse collapse" data-bs-parent="#accordionSolution1" aria-labelledby="headingSolution1">
 <div class="accordion-body">
 <div class="codewrapper sourceCode" id="cb49">
 <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i aria-hidden="true" data-feather="chevron-right"></i>
@@ -886,7 +886,7 @@ <h3 class="callout-title">Exercise 2<a class="anchor" aria-label="anchor" href="
 <button class="accordion-button solution-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseSolution2" aria-expanded="false" aria-controls="collapseSolution2">
   <h4 class="accordion-header" id="headingSolution2">Show me the solution</h4>
 </button>
-<div id="collapseSolution2" class="accordion-collapse collapse" aria-labelledby="headingSolution2" data-bs-parent="#accordionSolution2">
+<div id="collapseSolution2" class="accordion-collapse collapse" data-bs-parent="#accordionSolution2" aria-labelledby="headingSolution2">
 <div class="accordion-body">
 <div class="codewrapper sourceCode" id="cb50">
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@@ -920,7 +920,7 @@ <h3 class="callout-title">Exercise 3<a class="anchor" aria-label="anchor" href="
 <button class="accordion-button solution-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseSolution3" aria-expanded="false" aria-controls="collapseSolution3">
   <h4 class="accordion-header" id="headingSolution3">Show me the solution</h4>
 </button>
-<div id="collapseSolution3" class="accordion-collapse collapse" aria-labelledby="headingSolution3" data-bs-parent="#accordionSolution3">
+<div id="collapseSolution3" class="accordion-collapse collapse" data-bs-parent="#accordionSolution3" aria-labelledby="headingSolution3">
 <div class="accordion-body">
 <div class="codewrapper sourceCode" id="cb51">
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@@ -962,7 +962,7 @@ <h3 class="callout-title">Exercise 4<a class="anchor" aria-label="anchor" href="
 <button class="accordion-button solution-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseSolution4" aria-expanded="false" aria-controls="collapseSolution4">
   <h4 class="accordion-header" id="headingSolution4">Show me the solution</h4>
 </button>
-<div id="collapseSolution4" class="accordion-collapse collapse" aria-labelledby="headingSolution4" data-bs-parent="#accordionSolution4">
+<div id="collapseSolution4" class="accordion-collapse collapse" data-bs-parent="#accordionSolution4" aria-labelledby="headingSolution4">
 <div class="accordion-body">
 <div class="codewrapper sourceCode" id="cb52">
 <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i aria-hidden="true" data-feather="chevron-right"></i>
@@ -1120,8 +1120,8 @@ <h1 id="acknowledgements">Acknowledgements<a class="anchor" aria-label="anchor"
   "url": "https://ccb-hms.github.io/osca-carpentries/hca.html",
   "identifier": "https://ccb-hms.github.io/osca-carpentries/hca.html",
   "dateCreated": "2024-01-10",
-  "dateModified": "2024-03-25",
-  "datePublished": "2024-04-02"
+  "dateModified": "2024-04-09",
+  "datePublished": "2024-04-09"
 }
 
   </script><script>
diff --git a/images.html b/images.html
index c5a8dc1..90be9e0 100644
--- a/images.html
+++ b/images.html
@@ -424,8 +424,8 @@ <h3 id="multi-sample-figure-7">Figure 7</h3>
   "url": "https://ccb-hms.github.io/osca-carpentries/images.html",
   "identifier": "https://ccb-hms.github.io/osca-carpentries/images.html",
   "dateCreated": "2024-01-10",
-  "dateModified": "2024-04-02",
-  "datePublished": "2024-04-02"
+  "dateModified": "2024-04-09",
+  "datePublished": "2024-04-09"
 }
 
   </script><script>
diff --git a/index.html b/index.html
index a9cbf30..95fe584 100644
--- a/index.html
+++ b/index.html
@@ -386,8 +386,8 @@ <h4 class="accordion-header" id="headingSolution3">Linux</h4>
   "url": "https://ccb-hms.github.io/osca-carpentries/index.html",
   "identifier": "https://ccb-hms.github.io/osca-carpentries/index.html",
   "dateCreated": "2024-01-10",
-  "dateModified": "2024-04-02",
-  "datePublished": "2024-04-02"
+  "dateModified": "2024-04-09",
+  "datePublished": "2024-04-09"
 }
 
   </script><script>
diff --git a/instructor-notes.html b/instructor-notes.html
index 60faefa..2e26c12 100644
--- a/instructor-notes.html
+++ b/instructor-notes.html
@@ -360,8 +360,8 @@ <h1>Instructor Notes</h1>
   "url": "https://ccb-hms.github.io/osca-carpentries/instructor-notes.html",
   "identifier": "https://ccb-hms.github.io/osca-carpentries/instructor-notes.html",
   "dateCreated": "2024-01-10",
-  "dateModified": "2024-04-02",
-  "datePublished": "2024-04-02"
+  "dateModified": "2024-04-09",
+  "datePublished": "2024-04-09"
 }
 
   </script><script>
diff --git a/instructor/404.html b/instructor/404.html
index 2504e6e..cb72ebf 100644
--- a/instructor/404.html
+++ b/instructor/404.html
@@ -318,8 +318,8 @@ <h1>Page not found</h1>
   "url": "https://ccb-hms.github.io/osca-carpentries/instructor/404.html",
   "identifier": "https://ccb-hms.github.io/osca-carpentries/instructor/404.html",
   "dateCreated": "2024-01-10",
-  "dateModified": "2024-04-02",
-  "datePublished": "2024-04-02"
+  "dateModified": "2024-04-09",
+  "datePublished": "2024-04-09"
 }
 
   </script><script>
diff --git a/instructor/CODE_OF_CONDUCT.html b/instructor/CODE_OF_CONDUCT.html
index 76a0dd2..6e78a0c 100644
--- a/instructor/CODE_OF_CONDUCT.html
+++ b/instructor/CODE_OF_CONDUCT.html
@@ -256,7 +256,7 @@ <h2 class="accordion-header" id="flush-headingTwelve">
       </div>
       <hr></nav><main id="main-content" class="main-content"><div class="container lesson-content">
         <h1>Contributor Code of Conduct</h1>
-        <p>Last updated on 2024-03-25 |
+        <p>Last updated on 2024-04-09 |
         
         <a href="https://github.com/ccb-hms/osca-carpentries/edit/main/CODE_OF_CONDUCT.md" class="external-link">Edit this page <i aria-hidden="true" data-feather="edit"></i></a></p>
         
@@ -341,8 +341,8 @@ <h1>Contributor Code of Conduct</h1>
   "url": "https://ccb-hms.github.io/osca-carpentries/instructor/CODE_OF_CONDUCT.html",
   "identifier": "https://ccb-hms.github.io/osca-carpentries/instructor/CODE_OF_CONDUCT.html",
   "dateCreated": "2024-01-10",
-  "dateModified": "2024-03-25",
-  "datePublished": "2024-04-02"
+  "dateModified": "2024-04-09",
+  "datePublished": "2024-04-09"
 }
 
   </script><script>
diff --git a/instructor/LICENSE.html b/instructor/LICENSE.html
index f71990a..f1d8bf6 100644
--- a/instructor/LICENSE.html
+++ b/instructor/LICENSE.html
@@ -256,7 +256,7 @@ <h2 class="accordion-header" id="flush-headingTwelve">
       </div>
       <hr></nav><main id="main-content" class="main-content"><div class="container lesson-content">
         <h1>Licenses</h1>
-        <p>Last updated on 2024-03-25 |
+        <p>Last updated on 2024-04-09 |
         
         <a href="https://github.com/ccb-hms/osca-carpentries/edit/main/LICENSE.md" class="external-link">Edit this page <i aria-hidden="true" data-feather="edit"></i></a></p>
         
@@ -392,8 +392,8 @@ <h1>Licenses</h1>
   "url": "https://ccb-hms.github.io/osca-carpentries/instructor/LICENSE.html",
   "identifier": "https://ccb-hms.github.io/osca-carpentries/instructor/LICENSE.html",
   "dateCreated": "2024-01-10",
-  "dateModified": "2024-03-25",
-  "datePublished": "2024-04-02"
+  "dateModified": "2024-04-09",
+  "datePublished": "2024-04-09"
 }
 
   </script><script>
diff --git a/instructor/aio.html b/instructor/aio.html
index dcf94c1..cccfa12 100644
--- a/instructor/aio.html
+++ b/instructor/aio.html
@@ -308,7 +308,7 @@ <h2 class="accordion-header" id="flush-headingTwelve">
         
 <section id="aio-intro-sce"><p>Content from <a href="intro-sce.html">Introduction to Bioconductor and the SingleCellExperiment class</a></p>
 <hr>
-<p>Last updated on 2024-03-25 |
+<p>Last updated on 2024-04-09 |
         
         <a href="https://github.com/ccb-hms/osca-carpentries/edit/main/episodes/intro-sce.Rmd" class="external-link">Edit this page <i aria-hidden="true" data-feather="edit"></i></a></p>
 <p>Estimated time: <i aria-hidden="true" data-feather="clock"></i> 30 minutes</p>
@@ -781,7 +781,7 @@ <h3 class="callout-title">Exercise 1<a class="anchor" aria-label="anchor" href="
 <button class="accordion-button hint-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseHint1" aria-expanded="false" aria-controls="collapseHint1">
   <h4 class="accordion-header" id="headingHint1">Give me a hint</h4>
 </button>
-<div id="collapseHint1" class="accordion-collapse collapse" data-bs-parent="#accordionHint1" aria-labelledby="headingHint1">
+<div id="collapseHint1" class="accordion-collapse collapse" aria-labelledby="headingHint1" data-bs-parent="#accordionHint1">
 <div class="accordion-body">
 <p>The <code>SingleCellExperiment</code> function can be used to create
 a new SingleCellExperiment object</p>
@@ -794,7 +794,7 @@ <h4 class="accordion-header" id="headingHint1">Give me a hint</h4>
 <button class="accordion-button solution-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseSolution1" aria-expanded="false" aria-controls="collapseSolution1">
   <h4 class="accordion-header" id="headingSolution1">Show me the solution</h4>
 </button>
-<div id="collapseSolution1" class="accordion-collapse collapse" data-bs-parent="#accordionSolution1" aria-labelledby="headingSolution1">
+<div id="collapseSolution1" class="accordion-collapse collapse" aria-labelledby="headingSolution1" data-bs-parent="#accordionSolution1">
 <div class="accordion-body">
 <p>TODO</p>
 </div>
@@ -822,7 +822,7 @@ <h3 class="callout-title">Exercise 2<a class="anchor" aria-label="anchor" href="
 <button class="accordion-button solution-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseSolution2" aria-expanded="false" aria-controls="collapseSolution2">
   <h4 class="accordion-header" id="headingSolution2">Show me the solution</h4>
 </button>
-<div id="collapseSolution2" class="accordion-collapse collapse" data-bs-parent="#accordionSolution2" aria-labelledby="headingSolution2">
+<div id="collapseSolution2" class="accordion-collapse collapse" aria-labelledby="headingSolution2" data-bs-parent="#accordionSolution2">
 <div class="accordion-body">
 <p>TODO</p>
 </div>
@@ -865,7 +865,7 @@ <h1 id="further-reading">Further Reading<a class="anchor" aria-label="anchor" hr
  -->
 </div></section><section id="aio-eda_qc"><p>Content from <a href="eda_qc.html">Exploratory data analysis and quality control</a></p>
 <hr>
-<p>Last updated on 2024-03-25 |
+<p>Last updated on 2024-04-09 |
         
         <a href="https://github.com/ccb-hms/osca-carpentries/edit/main/episodes/eda_qc.Rmd" class="external-link">Edit this page <i aria-hidden="true" data-feather="edit"></i></a></p>
 <p>Estimated time: <i aria-hidden="true" data-feather="clock"></i> 12 minutes</p>
@@ -2031,7 +2031,7 @@ <h3 class="callout-title">Exercise 1: Normalization<a class="anchor" aria-label=
 <button class="accordion-button solution-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseSolution1" aria-expanded="false" aria-controls="collapseSolution1">
   <h4 class="accordion-header" id="headingSolution1">Show me the solution</h4>
 </button>
-<div id="collapseSolution1" class="accordion-collapse collapse" data-bs-parent="#accordionSolution1" aria-labelledby="headingSolution1">
+<div id="collapseSolution1" class="accordion-collapse collapse" aria-labelledby="headingSolution1" data-bs-parent="#accordionSolution1">
 <div class="accordion-body">
 <p>TODO</p>
 </div>
@@ -2059,7 +2059,7 @@ <h3 class="callout-title">Exercise 2: Dimensionality Reduction<a class="anchor"
 <button class="accordion-button hint-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseHint1" aria-expanded="false" aria-controls="collapseHint1">
   <h4 class="accordion-header" id="headingHint1">Give me a hint</h4>
 </button>
-<div id="collapseHint1" class="accordion-collapse collapse" data-bs-parent="#accordionHint1" aria-labelledby="headingHint1">
+<div id="collapseHint1" class="accordion-collapse collapse" aria-labelledby="headingHint1" data-bs-parent="#accordionHint1">
 <div class="accordion-body">
 <p>First subset the object to include only highly variable genes
 (<code>sce2 &lt;- sce[hvg.sce.var,]</code>) and then apply the
@@ -2074,7 +2074,7 @@ <h4 class="accordion-header" id="headingHint1">Give me a hint</h4>
 <button class="accordion-button solution-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseSolution2" aria-expanded="false" aria-controls="collapseSolution2">
   <h4 class="accordion-header" id="headingSolution2">Show me the solution</h4>
 </button>
-<div id="collapseSolution2" class="accordion-collapse collapse" data-bs-parent="#accordionSolution2" aria-labelledby="headingSolution2">
+<div id="collapseSolution2" class="accordion-collapse collapse" aria-labelledby="headingSolution2" data-bs-parent="#accordionSolution2">
 <div class="accordion-body">
 <p>TODO</p>
 </div>
@@ -2101,7 +2101,7 @@ <h3 class="callout-title">Exercise 3: PBMC Data<a class="anchor" aria-label="anc
 <button class="accordion-button solution-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseSolution3" aria-expanded="false" aria-controls="collapseSolution3">
   <h4 class="accordion-header" id="headingSolution3">Show me the solution</h4>
 </button>
-<div id="collapseSolution3" class="accordion-collapse collapse" data-bs-parent="#accordionSolution3" aria-labelledby="headingSolution3">
+<div id="collapseSolution3" class="accordion-collapse collapse" aria-labelledby="headingSolution3" data-bs-parent="#accordionSolution3">
 <div class="accordion-body">
 <p>TODO</p>
 </div>
@@ -2132,7 +2132,7 @@ <h1 id="references">References<a class="anchor" aria-label="anchor" href="#refer
  -->
 </div></section><section id="aio-cell_type_annotation"><p>Content from <a href="cell_type_annotation.html">Cell type annotation</a></p>
 <hr>
-<p>Last updated on 2024-03-25 |
+<p>Last updated on 2024-04-09 |
         
         <a href="https://github.com/ccb-hms/osca-carpentries/edit/main/episodes/cell_type_annotation.Rmd" class="external-link">Edit this page <i aria-hidden="true" data-feather="edit"></i></a></p>
 <p>Estimated time: <i aria-hidden="true" data-feather="clock"></i> 12 minutes</p>
@@ -3348,7 +3348,7 @@ <h3 class="callout-title">Key Points<a class="anchor" aria-label="anchor" href="
  -->
 </div></section><section id="aio-multi-sample"><p>Content from <a href="multi-sample.html">Multi-sample analyses</a></p>
 <hr>
-<p>Last updated on 2024-03-25 |
+<p>Last updated on 2024-04-09 |
         
         <a href="https://github.com/ccb-hms/osca-carpentries/edit/main/episodes/multi-sample.Rmd" class="external-link">Edit this page <i aria-hidden="true" data-feather="edit"></i></a></p>
 <p>Estimated time: <i aria-hidden="true" data-feather="clock"></i> 12 minutes</p>
@@ -4419,7 +4419,7 @@ <h3 class="callout-title">Key Points<a class="anchor" aria-label="anchor" href="
  -->
 </div></section><section id="aio-large_data"><p>Content from <a href="large_data.html">Working with large data</a></p>
 <hr>
-<p>Last updated on 2024-03-25 |
+<p>Last updated on 2024-04-09 |
         
         <a href="https://github.com/ccb-hms/osca-carpentries/edit/main/episodes/large_data.Rmd" class="external-link">Edit this page <i aria-hidden="true" data-feather="edit"></i></a></p>
 <p>Estimated time: <i aria-hidden="true" data-feather="clock"></i> 12 minutes</p>
@@ -4886,24 +4886,20 @@ <h3 class="code-label">OUTPUT<i aria-hidden="true" data-feather="chevron-left"><
 <pre class="output" tabindex="0"><code>     approx
 exact   1   2   3   4   5   6   7   8   9  10  11  12  13  14  15
    1   90   0   0   0   0   0   0   0   1   0   0   0   0   0   0
-   2    0 143   0   1   0   0   0   0   0   0   0   0   0   0   0
+   2    0 143   0   0   0   0   0   0   0   0   0   0   0   0   1
    3    0   0  75   0   0   0   0   0   0   0   0   0   0   0   0
-   4    0   0   0 253   0   0   0   0   0   0   0   0 144   0   0
-   5    0   0   0   0  71   0   0  95   0   0   0   5   0   0   0
-   6    0   0   0   0   0 210   0   0   0   0   0   0   0   0   0
-   7    0   0   0   0   0   1 244   0   0   1   0   0   0   0   0
-   8    1   0   0   0   1   0   0   0 106   0   0   0   0   0   0
-   9    0   0   0   0   0   0   0   0   0 112   0  10   0   0   0
-   10   0   0   0   0   0   1   0   0   0   1 145   0   0   6   0
-   11   0   0   0   0   3   0   0   0   0   0   0 197   0   0   0
-   12   0   0   2   0 324   0   0   0   0   1   0   0   0   0   0
-   13   0   0   0   0   0   0   0   0   0   1   0   0   0 146   0
-   14   0   0   0   0   0   0   0   0   0   0   0   0   0   0  20</code></pre>
-</div>
-<div class="codewrapper">
-<h3 class="code-label">ERROR<i aria-hidden="true" data-feather="chevron-left"></i><i aria-hidden="true" data-feather="chevron-right"></i>
-</h3>
-<pre class="error" tabindex="0"><code>Error: rand &gt; 0.85 is not TRUE</code></pre>
+   4    0   0   0 341   0   0   0   0   0   0   0   0   0   0   0
+   5    1   0   0   0 392   0   0   0   1   1   0   3   0   0   0
+   6    0   0   0   0   0 209   0   0   0   0   0   0   0   0   0
+   7    0   0   0   0   0   0 245   0   0   1   0   0   0   0   0
+   8    0   0   0   0   1   0   0  95   0   0   0   0   0   0   0
+   9    1   0   0   0   1   0   0   0 106   0   0   0   0   0   0
+   10   0   0   0   0   0   0   0   0   0 112   4   0   1   0   0
+   11   0   0   0   0   0   1   0   0   0   0 143   0   0   0   0
+   12   0   0   0   0   3   0   0   0   0  10   0 201   0   0   0
+   13   0   0   0   0   0   0   0   0   0   0   6   0 146   0   0
+   14   0   0   0   0   0   0   0   0   0   0   0   0   0  20   0
+   15   0   0   0   0   0   0   0   0   0   0   0   0   0   0  56</code></pre>
 </div>
 <p>Note that Annoy writes the NN index to disk prior to performing the
 search. Thus, it may not actually be faster than the default exact
@@ -4912,7 +4908,7 @@ <h3 class="code-label">ERROR<i aria-hidden="true" data-feather="chevron-left"></
 also not difficult to find situations where the approximation
 deteriorates, especially at high dimensions, though this may not have an
 appreciable impact on the biological conclusions.</p>
-<div class="codewrapper sourceCode" id="cb30">
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 </h3>
 <pre class="downlit sourceCode r">
@@ -4947,7 +4943,7 @@ <h2 id="singular-value-decomposition">Singular value decomposition<a class="anch
 change the SVD algorithm used in any of these functions by simply
 specifying an alternative value for the <code>BSPARAM</code>
 argument.</p>
-<div class="codewrapper sourceCode" id="cb32">
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 </h3>
 <pre class="downlit sourceCode r">
@@ -4973,7 +4969,7 @@ <h3 class="code-label">OUTPUT<i aria-hidden="true" data-feather="chevron-left"><
   .. ..$ : chr [1:500] "ENSMUSG00000055609" "ENSMUSG00000052217" "ENSMUSG00000069919" "ENSMUSG00000048583" ...
   .. ..$ : chr [1:20] "PC1" "PC2" "PC3" "PC4" ...</code></pre>
 </div>
-<div class="codewrapper sourceCode" id="cb34">
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 </h3>
 <pre class="downlit sourceCode r">
@@ -5022,7 +5018,7 @@ <h2 id="seurat">Seurat<a class="anchor" aria-label="anchor" href="#seurat"></a>
 types of single-cell data. Seurat is developed and maintained by the <a href="https://satijalab.org/seurat/authors.html" class="external-link">Satija lab</a> and is
 released under the <a href="https://opensource.org/license/mit/" class="external-link">MIT
 license</a>.</p>
-<div class="codewrapper sourceCode" id="cb36">
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 </h3>
 <pre class="downlit sourceCode r">
@@ -5044,7 +5040,7 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 with functionality from OSCA/Bioconductor. We therefore need to first
 install the <a href="https://github.com/satijalab/seurat-data" class="external-link">SeuratData</a> package,
 which is available from GitHub only.</p>
-<div class="codewrapper sourceCode" id="cb37">
+<div class="codewrapper sourceCode" id="cb36">
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 <pre class="downlit sourceCode r">
@@ -5053,7 +5049,7 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 </div>
 <p>We then proceed by loading all required packages and installing the
 PBMC dataset:</p>
-<div class="codewrapper sourceCode" id="cb38">
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 <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i aria-hidden="true" data-feather="chevron-right"></i>
 </h3>
 <pre class="downlit sourceCode r">
@@ -5062,7 +5058,7 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 </div>
 <p>We then load the dataset as an <code>SeuratObject</code> and convert
 it to a <code>SingleCellExperiment</code>.</p>
-<div class="codewrapper sourceCode" id="cb39">
+<div class="codewrapper sourceCode" id="cb38">
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 </h3>
 <pre class="downlit sourceCode r">
@@ -5076,7 +5072,7 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 <p>Seurat also allows conversion from <code>SingleCellExperiment</code>
 objects to Seurat objects; we demonstrate this here on the wild-type
 chimera mouse gastrulation dataset.</p>
-<div class="codewrapper sourceCode" id="cb40">
+<div class="codewrapper sourceCode" id="cb39">
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 </h3>
 <pre class="downlit sourceCode r">
@@ -5087,7 +5083,7 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 </div>
 <p>After some processing of the dataset, the actual conversion is
 carried out with the <code>as.Seurat</code> function.</p>
-<div class="codewrapper sourceCode" id="cb41">
+<div class="codewrapper sourceCode" id="cb40">
 <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i aria-hidden="true" data-feather="chevron-right"></i>
 </h3>
 <pre class="downlit sourceCode r">
@@ -5120,7 +5116,7 @@ <h2 id="scanpy">Scanpy<a class="anchor" aria-label="anchor" href="#scanpy"></a>
 to enable users and developers to easily move data between these
 frameworks to construct a multi-language analysis pipeline across
 R/Bioconductor and Python.</p>
-<div class="codewrapper sourceCode" id="cb42">
+<div class="codewrapper sourceCode" id="cb41">
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 </h3>
 <pre class="downlit sourceCode r">
@@ -5130,7 +5126,7 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 <code>SingleCellExperiment</code> from an H5AD file. Here, we use an
 example H5AD file contained in the <em><a href="https://bioconductor.org/packages/3.18/zellkonverter" class="external-link">zellkonverter</a></em>
 package.</p>
-<div class="codewrapper sourceCode" id="cb43">
+<div class="codewrapper sourceCode" id="cb42">
 <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i aria-hidden="true" data-feather="chevron-right"></i>
 </h3>
 <pre class="downlit sourceCode r">
@@ -5178,7 +5174,7 @@ <h3 class="code-label">OUTPUT<i aria-hidden="true" data-feather="chevron-left"><
 <p>We can also write a <code>SingleCellExperiment</code> to an H5AD file
 with the <code>writeH5AD()</code> function. This is demonstrated below
 on the wild-type chimera mouse gastrulation dataset.</p>
-<div class="codewrapper sourceCode" id="cb49">
+<div class="codewrapper sourceCode" id="cb48">
 <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i aria-hidden="true" data-feather="chevron-right"></i>
 </h3>
 <pre class="downlit sourceCode r">
@@ -5199,7 +5195,7 @@ <h3 class="code-label">OUTPUT<i aria-hidden="true" data-feather="chevron-left"><
 <div class="section level1">
 <h1 id="session-info">Session Info<a class="anchor" aria-label="anchor" href="#session-info"></a>
 </h1>
-<div class="codewrapper sourceCode" id="cb51">
+<div class="codewrapper sourceCode" id="cb50">
 <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i aria-hidden="true" data-feather="chevron-right"></i>
 </h3>
 <pre class="downlit sourceCode r">
@@ -5362,7 +5358,7 @@ <h3 class="callout-title">Exercise 1: Out of memory representation<a class="anch
 <button class="accordion-button hint-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseHint1" aria-expanded="false" aria-controls="collapseHint1">
   <h4 class="accordion-header" id="headingHint1">Give me a hint</h4>
 </button>
-<div id="collapseHint1" class="accordion-collapse collapse" aria-labelledby="headingHint1" data-bs-parent="#accordionHint1">
+<div id="collapseHint1" class="accordion-collapse collapse" data-bs-parent="#accordionHint1" aria-labelledby="headingHint1">
 <div class="accordion-body">
 <p>See the <code>HDF5Array</code> function for reading from HDF5 and the
 <code>writeHDF5Array</code> function for writing to HDF5 from the <em><a href="https://bioconductor.org/packages/3.18/HDF5Array" class="external-link">HDF5Array</a></em>
@@ -5376,7 +5372,7 @@ <h4 class="accordion-header" id="headingHint1">Give me a hint</h4>
 <button class="accordion-button solution-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseSolution1" aria-expanded="false" aria-controls="collapseSolution1">
   <h4 class="accordion-header" id="headingSolution1">Show me the solution</h4>
 </button>
-<div id="collapseSolution1" class="accordion-collapse collapse" aria-labelledby="headingSolution1" data-bs-parent="#accordionSolution1">
+<div id="collapseSolution1" class="accordion-collapse collapse" data-bs-parent="#accordionSolution1" aria-labelledby="headingSolution1">
 <div class="accordion-body">
 <p>TODO</p>
 </div>
@@ -5404,7 +5400,7 @@ <h3 class="callout-title">Exercise 2: Parallelization<a class="anchor" aria-labe
 <button class="accordion-button hint-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseHint2" aria-expanded="false" aria-controls="collapseHint2">
   <h4 class="accordion-header" id="headingHint2">Give me a hint</h4>
 </button>
-<div id="collapseHint2" class="accordion-collapse collapse" aria-labelledby="headingHint2" data-bs-parent="#accordionHint2">
+<div id="collapseHint2" class="accordion-collapse collapse" data-bs-parent="#accordionHint2" aria-labelledby="headingHint2">
 <div class="accordion-body">
 <p>Use the function <code>system.time</code> to obtain the runtime of
 each job.</p>
@@ -5417,7 +5413,7 @@ <h4 class="accordion-header" id="headingHint2">Give me a hint</h4>
 <button class="accordion-button solution-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseSolution2" aria-expanded="false" aria-controls="collapseSolution2">
   <h4 class="accordion-header" id="headingSolution2">Show me the solution</h4>
 </button>
-<div id="collapseSolution2" class="accordion-collapse collapse" aria-labelledby="headingSolution2" data-bs-parent="#accordionSolution2">
+<div id="collapseSolution2" class="accordion-collapse collapse" data-bs-parent="#accordionSolution2" aria-labelledby="headingSolution2">
 <div class="accordion-body">
 <p>TODO</p>
 </div>
@@ -5447,7 +5443,7 @@ <h3 class="callout-title">Exercise 3: Conversion to Seurat<a class="anchor" aria
 <button class="accordion-button hint-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseHint3" aria-expanded="false" aria-controls="collapseHint3">
   <h4 class="accordion-header" id="headingHint3">Give me a hint</h4>
 </button>
-<div id="collapseHint3" class="accordion-collapse collapse" aria-labelledby="headingHint3" data-bs-parent="#accordionHint3">
+<div id="collapseHint3" class="accordion-collapse collapse" data-bs-parent="#accordionHint3" aria-labelledby="headingHint3">
 <div class="accordion-body">
 <p>Use Seurat’s <code>DimPlot</code> function.</p>
 </div>
@@ -5459,7 +5455,7 @@ <h4 class="accordion-header" id="headingHint3">Give me a hint</h4>
 <button class="accordion-button solution-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseSolution3" aria-expanded="false" aria-controls="collapseSolution3">
   <h4 class="accordion-header" id="headingSolution3">Show me the solution</h4>
 </button>
-<div id="collapseSolution3" class="accordion-collapse collapse" aria-labelledby="headingSolution3" data-bs-parent="#accordionSolution3">
+<div id="collapseSolution3" class="accordion-collapse collapse" data-bs-parent="#accordionSolution3" aria-labelledby="headingSolution3">
 <div class="accordion-body">
 <p>Use Seurat’s <code>DimPlot</code> function.</p>
 </div>
@@ -5490,7 +5486,7 @@ <h1 id="references">References<a class="anchor" aria-label="anchor" href="#refer
  -->
 </div></section><section id="aio-hca"><p>Content from <a href="hca.html">Accessing data from the Human Cell Atlas (HCA)</a></p>
 <hr>
-<p>Last updated on 2024-03-25 |
+<p>Last updated on 2024-04-09 |
         
         <a href="https://github.com/ccb-hms/osca-carpentries/edit/main/episodes/hca.Rmd" class="external-link">Edit this page <i aria-hidden="true" data-feather="edit"></i></a></p>
 <p>Estimated time: <i aria-hidden="true" data-feather="clock"></i> 30 minutes</p>
@@ -5683,7 +5679,7 @@ <h3 class="code-label">OUTPUT<i aria-hidden="true" data-feather="chevron-left"><
 </h3>
 <pre class="output" tabindex="0"><code>Rows: ??
 Columns: 10
-Database: DuckDB v0.10.0 [unknown@Linux 6.5.0-1016-azure:R 4.3.3/:memory:]
+Database: DuckDB v0.10.1 [unknown@Linux 6.5.0-1017-azure:R 4.3.3/:memory:]
 $ cell_                             &lt;chr&gt; "TTATGCTAGGGTGTTG_12", "GCTTGAACATGG…
 $ sample_                           &lt;chr&gt; "039c558ca1c43dc74c563b58fe0d6289", …
 $ cell_type                         &lt;chr&gt; "mature NK T cell", "mature NK T cel…
@@ -5746,7 +5742,7 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 <h3 class="code-label">OUTPUT<i aria-hidden="true" data-feather="chevron-left"></i><i aria-hidden="true" data-feather="chevron-right"></i>
 </h3>
 <pre class="output" tabindex="0"><code># Source:   SQL [?? x 2]
-# Database: DuckDB v0.10.0 [unknown@Linux 6.5.0-1016-azure:R 4.3.3/:memory:]
+# Database: DuckDB v0.10.1 [unknown@Linux 6.5.0-1017-azure:R 4.3.3/:memory:]
    tissue                          n
    &lt;chr&gt;                       &lt;dbl&gt;
  1 renal medulla                   6
@@ -5798,19 +5794,19 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 <h3 class="code-label">OUTPUT<i aria-hidden="true" data-feather="chevron-left"></i><i aria-hidden="true" data-feather="chevron-right"></i>
 </h3>
 <pre class="output" tabindex="0"><code># Source:   SQL [?? x 2]
-# Database: DuckDB v0.10.0 [unknown@Linux 6.5.0-1016-azure:R 4.3.3/:memory:]
-   assay                              n
-   &lt;chr&gt;                          &lt;dbl&gt;
- 1 10x 3' v3                         21
- 2 Slide-seq                          4
- 3 10x 3' v2                         27
- 4 Visium Spatial Gene Expression     7
- 5 10x 5' v1                          7
- 6 scRNA-seq                          4
- 7 Seq-Well                           2
- 8 10x 5' v2                          2
- 9 10x 3' v1                          1
-10 Smart-seq2                         1
+# Database: DuckDB v0.10.1 [unknown@Linux 6.5.0-1017-azure:R 4.3.3/:memory:]
+   assay           n
+   &lt;chr&gt;       &lt;dbl&gt;
+ 1 10x 5' v2       2
+ 2 sci-RNA-seq     1
+ 3 10x 3' v1       1
+ 4 Smart-seq2      1
+ 5 10x 3' v3      21
+ 6 Slide-seq       4
+ 7 scRNA-seq       4
+ 8 Seq-Well        2
+ 9 10x 3' v2      27
+10 10x 5' v1       7
 # ℹ more rows</code></pre>
 </div>
 </div>
@@ -5829,7 +5825,7 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 <h3 class="code-label">OUTPUT<i aria-hidden="true" data-feather="chevron-left"></i><i aria-hidden="true" data-feather="chevron-right"></i>
 </h3>
 <pre class="output" tabindex="0"><code># Source:   SQL [1 x 2]
-# Database: DuckDB v0.10.0 [unknown@Linux 6.5.0-1016-azure:R 4.3.3/:memory:]
+# Database: DuckDB v0.10.1 [unknown@Linux 6.5.0-1017-azure:R 4.3.3/:memory:]
   organism         n
   &lt;chr&gt;        &lt;dbl&gt;
 1 Homo sapiens    63</code></pre>
@@ -6119,7 +6115,7 @@ <h3 class="callout-title">Exercise 1<a class="anchor" aria-label="anchor" href="
 <button class="accordion-button solution-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseSolution1" aria-expanded="false" aria-controls="collapseSolution1">
   <h4 class="accordion-header" id="headingSolution1">Show me the solution</h4>
 </button>
-<div id="collapseSolution1" class="accordion-collapse collapse" aria-labelledby="headingSolution1" data-bs-parent="#accordionSolution1">
+<div id="collapseSolution1" class="accordion-collapse collapse" data-bs-parent="#accordionSolution1" aria-labelledby="headingSolution1">
 <div class="accordion-body">
 <div class="codewrapper sourceCode" id="cb49">
 <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i aria-hidden="true" data-feather="chevron-right"></i>
@@ -6153,7 +6149,7 @@ <h3 class="callout-title">Exercise 2<a class="anchor" aria-label="anchor" href="
 <button class="accordion-button solution-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseSolution2" aria-expanded="false" aria-controls="collapseSolution2">
   <h4 class="accordion-header" id="headingSolution2">Show me the solution</h4>
 </button>
-<div id="collapseSolution2" class="accordion-collapse collapse" aria-labelledby="headingSolution2" data-bs-parent="#accordionSolution2">
+<div id="collapseSolution2" class="accordion-collapse collapse" data-bs-parent="#accordionSolution2" aria-labelledby="headingSolution2">
 <div class="accordion-body">
 <div class="codewrapper sourceCode" id="cb50">
 <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i aria-hidden="true" data-feather="chevron-right"></i>
@@ -6187,7 +6183,7 @@ <h3 class="callout-title">Exercise 3<a class="anchor" aria-label="anchor" href="
 <button class="accordion-button solution-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseSolution3" aria-expanded="false" aria-controls="collapseSolution3">
   <h4 class="accordion-header" id="headingSolution3">Show me the solution</h4>
 </button>
-<div id="collapseSolution3" class="accordion-collapse collapse" aria-labelledby="headingSolution3" data-bs-parent="#accordionSolution3">
+<div id="collapseSolution3" class="accordion-collapse collapse" data-bs-parent="#accordionSolution3" aria-labelledby="headingSolution3">
 <div class="accordion-body">
 <div class="codewrapper sourceCode" id="cb51">
 <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i aria-hidden="true" data-feather="chevron-right"></i>
@@ -6229,7 +6225,7 @@ <h3 class="callout-title">Exercise 4<a class="anchor" aria-label="anchor" href="
 <button class="accordion-button solution-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseSolution4" aria-expanded="false" aria-controls="collapseSolution4">
   <h4 class="accordion-header" id="headingSolution4">Show me the solution</h4>
 </button>
-<div id="collapseSolution4" class="accordion-collapse collapse" aria-labelledby="headingSolution4" data-bs-parent="#accordionSolution4">
+<div id="collapseSolution4" class="accordion-collapse collapse" data-bs-parent="#accordionSolution4" aria-labelledby="headingSolution4">
 <div class="accordion-body">
 <div class="codewrapper sourceCode" id="cb52">
 <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i aria-hidden="true" data-feather="chevron-right"></i>
@@ -6376,8 +6372,8 @@ <h1 id="acknowledgements">Acknowledgements<a class="anchor" aria-label="anchor"
   "url": "https://ccb-hms.github.io/osca-carpentries/instructor/aio.html",
   "identifier": "https://ccb-hms.github.io/osca-carpentries/instructor/aio.html",
   "dateCreated": "2024-01-10",
-  "dateModified": "2024-04-02",
-  "datePublished": "2024-04-02"
+  "dateModified": "2024-04-09",
+  "datePublished": "2024-04-09"
 }
 
   </script><script>
diff --git a/instructor/cell_type_annotation.html b/instructor/cell_type_annotation.html
index 9b095b4..4c186f1 100644
--- a/instructor/cell_type_annotation.html
+++ b/instructor/cell_type_annotation.html
@@ -260,7 +260,7 @@ <h2 class="accordion-header" id="flush-headingTwelve">
       </div>
       <hr></nav><main id="main-content" class="main-content"><div class="container lesson-content">
         <h1>Cell type annotation</h1>
-        <p>Last updated on 2024-03-25 |
+        <p>Last updated on 2024-04-09 |
         
         <a href="https://github.com/ccb-hms/osca-carpentries/edit/main/episodes/cell_type_annotation.Rmd" class="external-link">Edit this page <i aria-hidden="true" data-feather="edit"></i></a></p>
         
@@ -1521,8 +1521,8 @@ <h3 class="callout-title">Key Points<a class="anchor" aria-label="anchor" href="
   "url": "https://ccb-hms.github.io/osca-carpentries/instructor/cell_type_annotation.html",
   "identifier": "https://ccb-hms.github.io/osca-carpentries/instructor/cell_type_annotation.html",
   "dateCreated": "2024-01-10",
-  "dateModified": "2024-03-25",
-  "datePublished": "2024-04-02"
+  "dateModified": "2024-04-09",
+  "datePublished": "2024-04-09"
 }
 
   </script><script>
diff --git a/instructor/eda_qc.html b/instructor/eda_qc.html
index 30ddb15..d35721c 100644
--- a/instructor/eda_qc.html
+++ b/instructor/eda_qc.html
@@ -260,7 +260,7 @@ <h2 class="accordion-header" id="flush-headingTwelve">
       </div>
       <hr></nav><main id="main-content" class="main-content"><div class="container lesson-content">
         <h1>Exploratory data analysis and quality control</h1>
-        <p>Last updated on 2024-03-25 |
+        <p>Last updated on 2024-04-09 |
         
         <a href="https://github.com/ccb-hms/osca-carpentries/edit/main/episodes/eda_qc.Rmd" class="external-link">Edit this page <i aria-hidden="true" data-feather="edit"></i></a></p>
         
@@ -1399,7 +1399,7 @@ <h3 class="callout-title">Exercise 1: Normalization<a class="anchor" aria-label=
 <button class="accordion-button solution-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseSolution1" aria-expanded="false" aria-controls="collapseSolution1">
   <h4 class="accordion-header" id="headingSolution1">Show me the solution</h4>
 </button>
-<div id="collapseSolution1" class="accordion-collapse collapse" data-bs-parent="#accordionSolution1" aria-labelledby="headingSolution1">
+<div id="collapseSolution1" class="accordion-collapse collapse" aria-labelledby="headingSolution1" data-bs-parent="#accordionSolution1">
 <div class="accordion-body">
 <p>TODO</p>
 </div>
@@ -1427,7 +1427,7 @@ <h3 class="callout-title">Exercise 2: Dimensionality Reduction<a class="anchor"
 <button class="accordion-button hint-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseHint1" aria-expanded="false" aria-controls="collapseHint1">
   <h4 class="accordion-header" id="headingHint1">Give me a hint</h4>
 </button>
-<div id="collapseHint1" class="accordion-collapse collapse" data-bs-parent="#accordionHint1" aria-labelledby="headingHint1">
+<div id="collapseHint1" class="accordion-collapse collapse" aria-labelledby="headingHint1" data-bs-parent="#accordionHint1">
 <div class="accordion-body">
 <p>First subset the object to include only highly variable genes
 (<code>sce2 &lt;- sce[hvg.sce.var,]</code>) and then apply the
@@ -1442,7 +1442,7 @@ <h4 class="accordion-header" id="headingHint1">Give me a hint</h4>
 <button class="accordion-button solution-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseSolution2" aria-expanded="false" aria-controls="collapseSolution2">
   <h4 class="accordion-header" id="headingSolution2">Show me the solution</h4>
 </button>
-<div id="collapseSolution2" class="accordion-collapse collapse" data-bs-parent="#accordionSolution2" aria-labelledby="headingSolution2">
+<div id="collapseSolution2" class="accordion-collapse collapse" aria-labelledby="headingSolution2" data-bs-parent="#accordionSolution2">
 <div class="accordion-body">
 <p>TODO</p>
 </div>
@@ -1469,7 +1469,7 @@ <h3 class="callout-title">Exercise 3: PBMC Data<a class="anchor" aria-label="anc
 <button class="accordion-button solution-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseSolution3" aria-expanded="false" aria-controls="collapseSolution3">
   <h4 class="accordion-header" id="headingSolution3">Show me the solution</h4>
 </button>
-<div id="collapseSolution3" class="accordion-collapse collapse" data-bs-parent="#accordionSolution3" aria-labelledby="headingSolution3">
+<div id="collapseSolution3" class="accordion-collapse collapse" aria-labelledby="headingSolution3" data-bs-parent="#accordionSolution3">
 <div class="accordion-body">
 <p>TODO</p>
 </div>
@@ -1557,8 +1557,8 @@ <h1 id="references">References<a class="anchor" aria-label="anchor" href="#refer
   "url": "https://ccb-hms.github.io/osca-carpentries/instructor/eda_qc.html",
   "identifier": "https://ccb-hms.github.io/osca-carpentries/instructor/eda_qc.html",
   "dateCreated": "2024-01-10",
-  "dateModified": "2024-03-25",
-  "datePublished": "2024-04-02"
+  "dateModified": "2024-04-09",
+  "datePublished": "2024-04-09"
 }
 
   </script><script>
diff --git a/instructor/hca.html b/instructor/hca.html
index 845481f..604147b 100644
--- a/instructor/hca.html
+++ b/instructor/hca.html
@@ -260,7 +260,7 @@ <h2 class="accordion-header" id="flush-headingTwelve">
       </div>
       <hr></nav><main id="main-content" class="main-content"><div class="container lesson-content">
         <h1>Accessing data from the Human Cell Atlas (HCA)</h1>
-        <p>Last updated on 2024-03-25 |
+        <p>Last updated on 2024-04-09 |
         
         <a href="https://github.com/ccb-hms/osca-carpentries/edit/main/episodes/hca.Rmd" class="external-link">Edit this page <i aria-hidden="true" data-feather="edit"></i></a></p>
         
@@ -431,7 +431,7 @@ <h3 class="code-label">OUTPUT<i aria-hidden="true" data-feather="chevron-left"><
 </h3>
 <pre class="output" tabindex="0"><code>Rows: ??
 Columns: 10
-Database: DuckDB v0.10.0 [unknown@Linux 6.5.0-1016-azure:R 4.3.3/:memory:]
+Database: DuckDB v0.10.1 [unknown@Linux 6.5.0-1017-azure:R 4.3.3/:memory:]
 $ cell_                             &lt;chr&gt; "TTATGCTAGGGTGTTG_12", "GCTTGAACATGG…
 $ sample_                           &lt;chr&gt; "039c558ca1c43dc74c563b58fe0d6289", …
 $ cell_type                         &lt;chr&gt; "mature NK T cell", "mature NK T cel…
@@ -492,7 +492,7 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 <h3 class="code-label">OUTPUT<i aria-hidden="true" data-feather="chevron-left"></i><i aria-hidden="true" data-feather="chevron-right"></i>
 </h3>
 <pre class="output" tabindex="0"><code># Source:   SQL [?? x 2]
-# Database: DuckDB v0.10.0 [unknown@Linux 6.5.0-1016-azure:R 4.3.3/:memory:]
+# Database: DuckDB v0.10.1 [unknown@Linux 6.5.0-1017-azure:R 4.3.3/:memory:]
    tissue                          n
    &lt;chr&gt;                       &lt;dbl&gt;
  1 renal medulla                   6
@@ -542,19 +542,19 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 <h3 class="code-label">OUTPUT<i aria-hidden="true" data-feather="chevron-left"></i><i aria-hidden="true" data-feather="chevron-right"></i>
 </h3>
 <pre class="output" tabindex="0"><code># Source:   SQL [?? x 2]
-# Database: DuckDB v0.10.0 [unknown@Linux 6.5.0-1016-azure:R 4.3.3/:memory:]
-   assay                              n
-   &lt;chr&gt;                          &lt;dbl&gt;
- 1 10x 3' v3                         21
- 2 Slide-seq                          4
- 3 10x 3' v2                         27
- 4 Visium Spatial Gene Expression     7
- 5 10x 5' v1                          7
- 6 scRNA-seq                          4
- 7 Seq-Well                           2
- 8 10x 5' v2                          2
- 9 10x 3' v1                          1
-10 Smart-seq2                         1
+# Database: DuckDB v0.10.1 [unknown@Linux 6.5.0-1017-azure:R 4.3.3/:memory:]
+   assay           n
+   &lt;chr&gt;       &lt;dbl&gt;
+ 1 10x 5' v2       2
+ 2 sci-RNA-seq     1
+ 3 10x 3' v1       1
+ 4 Smart-seq2      1
+ 5 10x 3' v3      21
+ 6 Slide-seq       4
+ 7 scRNA-seq       4
+ 8 Seq-Well        2
+ 9 10x 3' v2      27
+10 10x 5' v1       7
 # ℹ more rows</code></pre>
 </div>
 </div>
@@ -572,7 +572,7 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 <h3 class="code-label">OUTPUT<i aria-hidden="true" data-feather="chevron-left"></i><i aria-hidden="true" data-feather="chevron-right"></i>
 </h3>
 <pre class="output" tabindex="0"><code># Source:   SQL [1 x 2]
-# Database: DuckDB v0.10.0 [unknown@Linux 6.5.0-1016-azure:R 4.3.3/:memory:]
+# Database: DuckDB v0.10.1 [unknown@Linux 6.5.0-1017-azure:R 4.3.3/:memory:]
   organism         n
   &lt;chr&gt;        &lt;dbl&gt;
 1 Homo sapiens    63</code></pre>
@@ -854,7 +854,7 @@ <h3 class="callout-title">Exercise 1<a class="anchor" aria-label="anchor" href="
 <button class="accordion-button solution-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseSolution1" aria-expanded="false" aria-controls="collapseSolution1">
   <h4 class="accordion-header" id="headingSolution1">Show me the solution</h4>
 </button>
-<div id="collapseSolution1" class="accordion-collapse collapse" aria-labelledby="headingSolution1" data-bs-parent="#accordionSolution1">
+<div id="collapseSolution1" class="accordion-collapse collapse" data-bs-parent="#accordionSolution1" aria-labelledby="headingSolution1">
 <div class="accordion-body">
 <div class="codewrapper sourceCode" id="cb49">
 <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i aria-hidden="true" data-feather="chevron-right"></i>
@@ -888,7 +888,7 @@ <h3 class="callout-title">Exercise 2<a class="anchor" aria-label="anchor" href="
 <button class="accordion-button solution-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseSolution2" aria-expanded="false" aria-controls="collapseSolution2">
   <h4 class="accordion-header" id="headingSolution2">Show me the solution</h4>
 </button>
-<div id="collapseSolution2" class="accordion-collapse collapse" aria-labelledby="headingSolution2" data-bs-parent="#accordionSolution2">
+<div id="collapseSolution2" class="accordion-collapse collapse" data-bs-parent="#accordionSolution2" aria-labelledby="headingSolution2">
 <div class="accordion-body">
 <div class="codewrapper sourceCode" id="cb50">
 <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i aria-hidden="true" data-feather="chevron-right"></i>
@@ -922,7 +922,7 @@ <h3 class="callout-title">Exercise 3<a class="anchor" aria-label="anchor" href="
 <button class="accordion-button solution-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseSolution3" aria-expanded="false" aria-controls="collapseSolution3">
   <h4 class="accordion-header" id="headingSolution3">Show me the solution</h4>
 </button>
-<div id="collapseSolution3" class="accordion-collapse collapse" aria-labelledby="headingSolution3" data-bs-parent="#accordionSolution3">
+<div id="collapseSolution3" class="accordion-collapse collapse" data-bs-parent="#accordionSolution3" aria-labelledby="headingSolution3">
 <div class="accordion-body">
 <div class="codewrapper sourceCode" id="cb51">
 <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i aria-hidden="true" data-feather="chevron-right"></i>
@@ -964,7 +964,7 @@ <h3 class="callout-title">Exercise 4<a class="anchor" aria-label="anchor" href="
 <button class="accordion-button solution-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseSolution4" aria-expanded="false" aria-controls="collapseSolution4">
   <h4 class="accordion-header" id="headingSolution4">Show me the solution</h4>
 </button>
-<div id="collapseSolution4" class="accordion-collapse collapse" aria-labelledby="headingSolution4" data-bs-parent="#accordionSolution4">
+<div id="collapseSolution4" class="accordion-collapse collapse" data-bs-parent="#accordionSolution4" aria-labelledby="headingSolution4">
 <div class="accordion-body">
 <div class="codewrapper sourceCode" id="cb52">
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@@ -1122,8 +1122,8 @@ <h1 id="acknowledgements">Acknowledgements<a class="anchor" aria-label="anchor"
   "url": "https://ccb-hms.github.io/osca-carpentries/instructor/hca.html",
   "identifier": "https://ccb-hms.github.io/osca-carpentries/instructor/hca.html",
   "dateCreated": "2024-01-10",
-  "dateModified": "2024-03-25",
-  "datePublished": "2024-04-02"
+  "dateModified": "2024-04-09",
+  "datePublished": "2024-04-09"
 }
 
   </script><script>
diff --git a/instructor/images.html b/instructor/images.html
index ee54ea6..e66da1a 100644
--- a/instructor/images.html
+++ b/instructor/images.html
@@ -426,8 +426,8 @@ <h3 id="multi-sample-figure-7">Figure 7</h3>
   "url": "https://ccb-hms.github.io/osca-carpentries/instructor/images.html",
   "identifier": "https://ccb-hms.github.io/osca-carpentries/instructor/images.html",
   "dateCreated": "2024-01-10",
-  "dateModified": "2024-04-02",
-  "datePublished": "2024-04-02"
+  "dateModified": "2024-04-09",
+  "datePublished": "2024-04-09"
 }
 
   </script><script>
diff --git a/instructor/index.html b/instructor/index.html
index 71dc15e..f5aa39b 100644
--- a/instructor/index.html
+++ b/instructor/index.html
@@ -451,8 +451,8 @@ <h4 class="accordion-header" id="headingSolution3">Linux</h4>
   "url": "https://ccb-hms.github.io/osca-carpentries/instructor/index.html",
   "identifier": "https://ccb-hms.github.io/osca-carpentries/instructor/index.html",
   "dateCreated": "2024-01-10",
-  "dateModified": "2024-04-02",
-  "datePublished": "2024-04-02"
+  "dateModified": "2024-04-09",
+  "datePublished": "2024-04-09"
 }
 
   </script><script>
diff --git a/instructor/instructor-notes.html b/instructor/instructor-notes.html
index f3fe642..3beec37 100644
--- a/instructor/instructor-notes.html
+++ b/instructor/instructor-notes.html
@@ -368,8 +368,8 @@ <h1>Instructor Notes</h1>
   "url": "https://ccb-hms.github.io/osca-carpentries/instructor/instructor-notes.html",
   "identifier": "https://ccb-hms.github.io/osca-carpentries/instructor/instructor-notes.html",
   "dateCreated": "2024-01-10",
-  "dateModified": "2024-04-02",
-  "datePublished": "2024-04-02"
+  "dateModified": "2024-04-09",
+  "datePublished": "2024-04-09"
 }
 
   </script><script>
diff --git a/instructor/intro-sce.html b/instructor/intro-sce.html
index f17df7f..7d7e0ac 100644
--- a/instructor/intro-sce.html
+++ b/instructor/intro-sce.html
@@ -260,7 +260,7 @@ <h2 class="accordion-header" id="flush-headingTwelve">
       </div>
       <hr></nav><main id="main-content" class="main-content"><div class="container lesson-content">
         <h1>Introduction to Bioconductor and the SingleCellExperiment class</h1>
-        <p>Last updated on 2024-03-25 |
+        <p>Last updated on 2024-04-09 |
         
         <a href="https://github.com/ccb-hms/osca-carpentries/edit/main/episodes/intro-sce.Rmd" class="external-link">Edit this page <i aria-hidden="true" data-feather="edit"></i></a></p>
         
@@ -726,7 +726,7 @@ <h3 class="callout-title">Exercise 1<a class="anchor" aria-label="anchor" href="
 <button class="accordion-button hint-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseHint1" aria-expanded="false" aria-controls="collapseHint1">
   <h4 class="accordion-header" id="headingHint1">Give me a hint</h4>
 </button>
-<div id="collapseHint1" class="accordion-collapse collapse" data-bs-parent="#accordionHint1" aria-labelledby="headingHint1">
+<div id="collapseHint1" class="accordion-collapse collapse" aria-labelledby="headingHint1" data-bs-parent="#accordionHint1">
 <div class="accordion-body">
 <p>The <code>SingleCellExperiment</code> function can be used to create
 a new SingleCellExperiment object</p>
@@ -739,7 +739,7 @@ <h4 class="accordion-header" id="headingHint1">Give me a hint</h4>
 <button class="accordion-button solution-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseSolution1" aria-expanded="false" aria-controls="collapseSolution1">
   <h4 class="accordion-header" id="headingSolution1">Show me the solution</h4>
 </button>
-<div id="collapseSolution1" class="accordion-collapse collapse" data-bs-parent="#accordionSolution1" aria-labelledby="headingSolution1">
+<div id="collapseSolution1" class="accordion-collapse collapse" aria-labelledby="headingSolution1" data-bs-parent="#accordionSolution1">
 <div class="accordion-body">
 <p>TODO</p>
 </div>
@@ -767,7 +767,7 @@ <h3 class="callout-title">Exercise 2<a class="anchor" aria-label="anchor" href="
 <button class="accordion-button solution-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseSolution2" aria-expanded="false" aria-controls="collapseSolution2">
   <h4 class="accordion-header" id="headingSolution2">Show me the solution</h4>
 </button>
-<div id="collapseSolution2" class="accordion-collapse collapse" data-bs-parent="#accordionSolution2" aria-labelledby="headingSolution2">
+<div id="collapseSolution2" class="accordion-collapse collapse" aria-labelledby="headingSolution2" data-bs-parent="#accordionSolution2">
 <div class="accordion-body">
 <p>TODO</p>
 </div>
@@ -864,8 +864,8 @@ <h1 id="further-reading">Further Reading<a class="anchor" aria-label="anchor" hr
   "url": "https://ccb-hms.github.io/osca-carpentries/instructor/intro-sce.html",
   "identifier": "https://ccb-hms.github.io/osca-carpentries/instructor/intro-sce.html",
   "dateCreated": "2024-01-10",
-  "dateModified": "2024-03-25",
-  "datePublished": "2024-04-02"
+  "dateModified": "2024-04-09",
+  "datePublished": "2024-04-09"
 }
 
   </script><script>
diff --git a/instructor/key-points.html b/instructor/key-points.html
index cba7dc3..0385446 100644
--- a/instructor/key-points.html
+++ b/instructor/key-points.html
@@ -385,8 +385,8 @@ <h2 class="accordion-header" id="flush-headingTwelve">
   "url": "https://ccb-hms.github.io/osca-carpentries/instructor/key-points.html",
   "identifier": "https://ccb-hms.github.io/osca-carpentries/instructor/key-points.html",
   "dateCreated": "2024-01-10",
-  "dateModified": "2024-04-02",
-  "datePublished": "2024-04-02"
+  "dateModified": "2024-04-09",
+  "datePublished": "2024-04-09"
 }
 
   </script><script>
diff --git a/instructor/large_data.html b/instructor/large_data.html
index 25546f0..b1772a1 100644
--- a/instructor/large_data.html
+++ b/instructor/large_data.html
@@ -260,7 +260,7 @@ <h2 class="accordion-header" id="flush-headingTwelve">
       </div>
       <hr></nav><main id="main-content" class="main-content"><div class="container lesson-content">
         <h1>Working with large data</h1>
-        <p>Last updated on 2024-03-25 |
+        <p>Last updated on 2024-04-09 |
         
         <a href="https://github.com/ccb-hms/osca-carpentries/edit/main/episodes/large_data.Rmd" class="external-link">Edit this page <i aria-hidden="true" data-feather="edit"></i></a></p>
         
@@ -724,24 +724,20 @@ <h3 class="code-label">OUTPUT<i aria-hidden="true" data-feather="chevron-left"><
 <pre class="output" tabindex="0"><code>     approx
 exact   1   2   3   4   5   6   7   8   9  10  11  12  13  14  15
    1   90   0   0   0   0   0   0   0   1   0   0   0   0   0   0
-   2    0 143   0   1   0   0   0   0   0   0   0   0   0   0   0
+   2    0 143   0   0   0   0   0   0   0   0   0   0   0   0   1
    3    0   0  75   0   0   0   0   0   0   0   0   0   0   0   0
-   4    0   0   0 253   0   0   0   0   0   0   0   0 144   0   0
-   5    0   0   0   0  71   0   0  95   0   0   0   5   0   0   0
-   6    0   0   0   0   0 210   0   0   0   0   0   0   0   0   0
-   7    0   0   0   0   0   1 244   0   0   1   0   0   0   0   0
-   8    1   0   0   0   1   0   0   0 106   0   0   0   0   0   0
-   9    0   0   0   0   0   0   0   0   0 112   0  10   0   0   0
-   10   0   0   0   0   0   1   0   0   0   1 145   0   0   6   0
-   11   0   0   0   0   3   0   0   0   0   0   0 197   0   0   0
-   12   0   0   2   0 324   0   0   0   0   1   0   0   0   0   0
-   13   0   0   0   0   0   0   0   0   0   1   0   0   0 146   0
-   14   0   0   0   0   0   0   0   0   0   0   0   0   0   0  20</code></pre>
-</div>
-<div class="codewrapper">
-<h3 class="code-label">ERROR<i aria-hidden="true" data-feather="chevron-left"></i><i aria-hidden="true" data-feather="chevron-right"></i>
-</h3>
-<pre class="error" tabindex="0"><code>Error: rand &gt; 0.85 is not TRUE</code></pre>
+   4    0   0   0 341   0   0   0   0   0   0   0   0   0   0   0
+   5    1   0   0   0 392   0   0   0   1   1   0   3   0   0   0
+   6    0   0   0   0   0 209   0   0   0   0   0   0   0   0   0
+   7    0   0   0   0   0   0 245   0   0   1   0   0   0   0   0
+   8    0   0   0   0   1   0   0  95   0   0   0   0   0   0   0
+   9    1   0   0   0   1   0   0   0 106   0   0   0   0   0   0
+   10   0   0   0   0   0   0   0   0   0 112   4   0   1   0   0
+   11   0   0   0   0   0   1   0   0   0   0 143   0   0   0   0
+   12   0   0   0   0   3   0   0   0   0  10   0 201   0   0   0
+   13   0   0   0   0   0   0   0   0   0   0   6   0 146   0   0
+   14   0   0   0   0   0   0   0   0   0   0   0   0   0  20   0
+   15   0   0   0   0   0   0   0   0   0   0   0   0   0   0  56</code></pre>
 </div>
 <p>Note that Annoy writes the NN index to disk prior to performing the
 search. Thus, it may not actually be faster than the default exact
@@ -750,7 +746,7 @@ <h3 class="code-label">ERROR<i aria-hidden="true" data-feather="chevron-left"></
 also not difficult to find situations where the approximation
 deteriorates, especially at high dimensions, though this may not have an
 appreciable impact on the biological conclusions.</p>
-<div class="codewrapper sourceCode" id="cb30">
+<div class="codewrapper sourceCode" id="cb29">
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 </h3>
 <pre class="downlit sourceCode r">
@@ -784,7 +780,7 @@ <h2 id="singular-value-decomposition">Singular value decomposition<a class="anch
 change the SVD algorithm used in any of these functions by simply
 specifying an alternative value for the <code>BSPARAM</code>
 argument.</p>
-<div class="codewrapper sourceCode" id="cb32">
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 </h3>
 <pre class="downlit sourceCode r">
@@ -810,7 +806,7 @@ <h3 class="code-label">OUTPUT<i aria-hidden="true" data-feather="chevron-left"><
   .. ..$ : chr [1:500] "ENSMUSG00000055609" "ENSMUSG00000052217" "ENSMUSG00000069919" "ENSMUSG00000048583" ...
   .. ..$ : chr [1:20] "PC1" "PC2" "PC3" "PC4" ...</code></pre>
 </div>
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 </h3>
 <pre class="downlit sourceCode r">
@@ -857,7 +853,7 @@ <h2 id="seurat">Seurat<a class="anchor" aria-label="anchor" href="#seurat"></a><
 types of single-cell data. Seurat is developed and maintained by the <a href="https://satijalab.org/seurat/authors.html" class="external-link">Satija lab</a> and is
 released under the <a href="https://opensource.org/license/mit/" class="external-link">MIT
 license</a>.</p>
-<div class="codewrapper sourceCode" id="cb36">
+<div class="codewrapper sourceCode" id="cb35">
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 </h3>
 <pre class="downlit sourceCode r">
@@ -879,7 +875,7 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 with functionality from OSCA/Bioconductor. We therefore need to first
 install the <a href="https://github.com/satijalab/seurat-data" class="external-link">SeuratData</a> package,
 which is available from GitHub only.</p>
-<div class="codewrapper sourceCode" id="cb37">
+<div class="codewrapper sourceCode" id="cb36">
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 <pre class="downlit sourceCode r">
@@ -888,7 +884,7 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 </div>
 <p>We then proceed by loading all required packages and installing the
 PBMC dataset:</p>
-<div class="codewrapper sourceCode" id="cb38">
+<div class="codewrapper sourceCode" id="cb37">
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@@ -897,7 +893,7 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 </div>
 <p>We then load the dataset as an <code>SeuratObject</code> and convert
 it to a <code>SingleCellExperiment</code>.</p>
-<div class="codewrapper sourceCode" id="cb39">
+<div class="codewrapper sourceCode" id="cb38">
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 <pre class="downlit sourceCode r">
@@ -911,7 +907,7 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 <p>Seurat also allows conversion from <code>SingleCellExperiment</code>
 objects to Seurat objects; we demonstrate this here on the wild-type
 chimera mouse gastrulation dataset.</p>
-<div class="codewrapper sourceCode" id="cb40">
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@@ -922,7 +918,7 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 </div>
 <p>After some processing of the dataset, the actual conversion is
 carried out with the <code>as.Seurat</code> function.</p>
-<div class="codewrapper sourceCode" id="cb41">
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@@ -954,7 +950,7 @@ <h2 id="scanpy">Scanpy<a class="anchor" aria-label="anchor" href="#scanpy"></a><
 to enable users and developers to easily move data between these
 frameworks to construct a multi-language analysis pipeline across
 R/Bioconductor and Python.</p>
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 <pre class="downlit sourceCode r">
@@ -964,7 +960,7 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 <code>SingleCellExperiment</code> from an H5AD file. Here, we use an
 example H5AD file contained in the <em><a href="https://bioconductor.org/packages/3.18/zellkonverter" class="external-link">zellkonverter</a></em>
 package.</p>
-<div class="codewrapper sourceCode" id="cb43">
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 </h3>
 <pre class="downlit sourceCode r">
@@ -1012,7 +1008,7 @@ <h3 class="code-label">OUTPUT<i aria-hidden="true" data-feather="chevron-left"><
 <p>We can also write a <code>SingleCellExperiment</code> to an H5AD file
 with the <code>writeH5AD()</code> function. This is demonstrated below
 on the wild-type chimera mouse gastrulation dataset.</p>
-<div class="codewrapper sourceCode" id="cb49">
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 <pre class="downlit sourceCode r">
@@ -1032,7 +1028,7 @@ <h3 class="code-label">OUTPUT<i aria-hidden="true" data-feather="chevron-left"><
 </div>
 <div class="section level1">
 <h1 id="session-info">Session Info<a class="anchor" aria-label="anchor" href="#session-info"></a></h1>
-<div class="codewrapper sourceCode" id="cb51">
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 <pre class="downlit sourceCode r">
@@ -1191,7 +1187,7 @@ <h3 class="callout-title">Exercise 1: Out of memory representation<a class="anch
 <button class="accordion-button hint-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseHint1" aria-expanded="false" aria-controls="collapseHint1">
   <h4 class="accordion-header" id="headingHint1">Give me a hint</h4>
 </button>
-<div id="collapseHint1" class="accordion-collapse collapse" aria-labelledby="headingHint1" data-bs-parent="#accordionHint1">
+<div id="collapseHint1" class="accordion-collapse collapse" data-bs-parent="#accordionHint1" aria-labelledby="headingHint1">
 <div class="accordion-body">
 <p>See the <code>HDF5Array</code> function for reading from HDF5 and the
 <code>writeHDF5Array</code> function for writing to HDF5 from the <em><a href="https://bioconductor.org/packages/3.18/HDF5Array" class="external-link">HDF5Array</a></em>
@@ -1205,7 +1201,7 @@ <h4 class="accordion-header" id="headingHint1">Give me a hint</h4>
 <button class="accordion-button solution-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseSolution1" aria-expanded="false" aria-controls="collapseSolution1">
   <h4 class="accordion-header" id="headingSolution1">Show me the solution</h4>
 </button>
-<div id="collapseSolution1" class="accordion-collapse collapse" aria-labelledby="headingSolution1" data-bs-parent="#accordionSolution1">
+<div id="collapseSolution1" class="accordion-collapse collapse" data-bs-parent="#accordionSolution1" aria-labelledby="headingSolution1">
 <div class="accordion-body">
 <p>TODO</p>
 </div>
@@ -1233,7 +1229,7 @@ <h3 class="callout-title">Exercise 2: Parallelization<a class="anchor" aria-labe
 <button class="accordion-button hint-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseHint2" aria-expanded="false" aria-controls="collapseHint2">
   <h4 class="accordion-header" id="headingHint2">Give me a hint</h4>
 </button>
-<div id="collapseHint2" class="accordion-collapse collapse" aria-labelledby="headingHint2" data-bs-parent="#accordionHint2">
+<div id="collapseHint2" class="accordion-collapse collapse" data-bs-parent="#accordionHint2" aria-labelledby="headingHint2">
 <div class="accordion-body">
 <p>Use the function <code>system.time</code> to obtain the runtime of
 each job.</p>
@@ -1246,7 +1242,7 @@ <h4 class="accordion-header" id="headingHint2">Give me a hint</h4>
 <button class="accordion-button solution-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseSolution2" aria-expanded="false" aria-controls="collapseSolution2">
   <h4 class="accordion-header" id="headingSolution2">Show me the solution</h4>
 </button>
-<div id="collapseSolution2" class="accordion-collapse collapse" aria-labelledby="headingSolution2" data-bs-parent="#accordionSolution2">
+<div id="collapseSolution2" class="accordion-collapse collapse" data-bs-parent="#accordionSolution2" aria-labelledby="headingSolution2">
 <div class="accordion-body">
 <p>TODO</p>
 </div>
@@ -1276,7 +1272,7 @@ <h3 class="callout-title">Exercise 3: Conversion to Seurat<a class="anchor" aria
 <button class="accordion-button hint-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseHint3" aria-expanded="false" aria-controls="collapseHint3">
   <h4 class="accordion-header" id="headingHint3">Give me a hint</h4>
 </button>
-<div id="collapseHint3" class="accordion-collapse collapse" aria-labelledby="headingHint3" data-bs-parent="#accordionHint3">
+<div id="collapseHint3" class="accordion-collapse collapse" data-bs-parent="#accordionHint3" aria-labelledby="headingHint3">
 <div class="accordion-body">
 <p>Use Seurat’s <code>DimPlot</code> function.</p>
 </div>
@@ -1288,7 +1284,7 @@ <h4 class="accordion-header" id="headingHint3">Give me a hint</h4>
 <button class="accordion-button solution-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseSolution3" aria-expanded="false" aria-controls="collapseSolution3">
   <h4 class="accordion-header" id="headingSolution3">Show me the solution</h4>
 </button>
-<div id="collapseSolution3" class="accordion-collapse collapse" aria-labelledby="headingSolution3" data-bs-parent="#accordionSolution3">
+<div id="collapseSolution3" class="accordion-collapse collapse" data-bs-parent="#accordionSolution3" aria-labelledby="headingSolution3">
 <div class="accordion-body">
 <p>Use Seurat’s <code>DimPlot</code> function.</p>
 </div>
@@ -1376,8 +1372,8 @@ <h1 id="references">References<a class="anchor" aria-label="anchor" href="#refer
   "url": "https://ccb-hms.github.io/osca-carpentries/instructor/large_data.html",
   "identifier": "https://ccb-hms.github.io/osca-carpentries/instructor/large_data.html",
   "dateCreated": "2024-01-10",
-  "dateModified": "2024-03-25",
-  "datePublished": "2024-04-02"
+  "dateModified": "2024-04-09",
+  "datePublished": "2024-04-09"
 }
 
   </script><script>
diff --git a/instructor/multi-sample.html b/instructor/multi-sample.html
index c9eb1ff..10e8867 100644
--- a/instructor/multi-sample.html
+++ b/instructor/multi-sample.html
@@ -260,7 +260,7 @@ <h2 class="accordion-header" id="flush-headingTwelve">
       </div>
       <hr></nav><main id="main-content" class="main-content"><div class="container lesson-content">
         <h1>Multi-sample analyses</h1>
-        <p>Last updated on 2024-03-25 |
+        <p>Last updated on 2024-04-09 |
         
         <a href="https://github.com/ccb-hms/osca-carpentries/edit/main/episodes/multi-sample.Rmd" class="external-link">Edit this page <i aria-hidden="true" data-feather="edit"></i></a></p>
         
@@ -1376,8 +1376,8 @@ <h3 class="callout-title">Key Points<a class="anchor" aria-label="anchor" href="
   "url": "https://ccb-hms.github.io/osca-carpentries/instructor/multi-sample.html",
   "identifier": "https://ccb-hms.github.io/osca-carpentries/instructor/multi-sample.html",
   "dateCreated": "2024-01-10",
-  "dateModified": "2024-03-25",
-  "datePublished": "2024-04-02"
+  "dateModified": "2024-04-09",
+  "datePublished": "2024-04-09"
 }
 
   </script><script>
diff --git a/instructor/profiles.html b/instructor/profiles.html
index 1ade7d4..99e5d3a 100644
--- a/instructor/profiles.html
+++ b/instructor/profiles.html
@@ -293,8 +293,8 @@ <h1>Learner Profiles</h1>
   "url": "https://ccb-hms.github.io/osca-carpentries/instructor/profiles.html",
   "identifier": "https://ccb-hms.github.io/osca-carpentries/instructor/profiles.html",
   "dateCreated": "2024-01-10",
-  "dateModified": "2024-04-02",
-  "datePublished": "2024-04-02"
+  "dateModified": "2024-04-09",
+  "datePublished": "2024-04-09"
 }
 
   </script><script>
diff --git a/instructor/reference.html b/instructor/reference.html
index b0520aa..1e0e7f4 100644
--- a/instructor/reference.html
+++ b/instructor/reference.html
@@ -256,7 +256,7 @@ <h2 class="accordion-header" id="flush-headingTwelve">
       </div>
       <hr></nav><main id="main-content" class="main-content"><div class="container lesson-content">
         <h1>Reference</h1>
-        <p>Last updated on 2024-03-25 |
+        <p>Last updated on 2024-04-09 |
         
         <a href="https://github.com/ccb-hms/osca-carpentries/edit/main/learners/reference.md" class="external-link">Edit this page <i aria-hidden="true" data-feather="edit"></i></a></p>
         
@@ -334,8 +334,8 @@ <h1>Reference</h1>
   "url": "https://ccb-hms.github.io/osca-carpentries/instructor/reference.html",
   "identifier": "https://ccb-hms.github.io/osca-carpentries/instructor/reference.html",
   "dateCreated": "2024-01-10",
-  "dateModified": "2024-03-25",
-  "datePublished": "2024-04-02"
+  "dateModified": "2024-04-09",
+  "datePublished": "2024-04-09"
 }
 
   </script><script>
diff --git a/intro-sce.html b/intro-sce.html
index e44e2a2..02ff0af 100644
--- a/intro-sce.html
+++ b/intro-sce.html
@@ -260,7 +260,7 @@ <h2 class="accordion-header" id="flush-headingTwelve">
       </div>
       <hr></nav><main id="main-content" class="main-content"><div class="container lesson-content">
         <h1>Introduction to Bioconductor and the SingleCellExperiment class</h1>
-        <p>Last updated on 2024-03-25 |
+        <p>Last updated on 2024-04-09 |
         
         <a href="https://github.com/ccb-hms/osca-carpentries/edit/main/episodes/intro-sce.Rmd" class="external-link">Edit this page <i aria-hidden="true" data-feather="edit"></i></a></p>
         
@@ -724,7 +724,7 @@ <h3 class="callout-title">Exercise 1<a class="anchor" aria-label="anchor" href="
 <button class="accordion-button hint-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseHint1" aria-expanded="false" aria-controls="collapseHint1">
   <h4 class="accordion-header" id="headingHint1">Give me a hint</h4>
 </button>
-<div id="collapseHint1" class="accordion-collapse collapse" data-bs-parent="#accordionHint1" aria-labelledby="headingHint1">
+<div id="collapseHint1" class="accordion-collapse collapse" aria-labelledby="headingHint1" data-bs-parent="#accordionHint1">
 <div class="accordion-body">
 <p>The <code>SingleCellExperiment</code> function can be used to create
 a new SingleCellExperiment object</p>
@@ -737,7 +737,7 @@ <h4 class="accordion-header" id="headingHint1">Give me a hint</h4>
 <button class="accordion-button solution-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseSolution1" aria-expanded="false" aria-controls="collapseSolution1">
   <h4 class="accordion-header" id="headingSolution1">Show me the solution</h4>
 </button>
-<div id="collapseSolution1" class="accordion-collapse collapse" data-bs-parent="#accordionSolution1" aria-labelledby="headingSolution1">
+<div id="collapseSolution1" class="accordion-collapse collapse" aria-labelledby="headingSolution1" data-bs-parent="#accordionSolution1">
 <div class="accordion-body">
 <p>TODO</p>
 </div>
@@ -765,7 +765,7 @@ <h3 class="callout-title">Exercise 2<a class="anchor" aria-label="anchor" href="
 <button class="accordion-button solution-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseSolution2" aria-expanded="false" aria-controls="collapseSolution2">
   <h4 class="accordion-header" id="headingSolution2">Show me the solution</h4>
 </button>
-<div id="collapseSolution2" class="accordion-collapse collapse" data-bs-parent="#accordionSolution2" aria-labelledby="headingSolution2">
+<div id="collapseSolution2" class="accordion-collapse collapse" aria-labelledby="headingSolution2" data-bs-parent="#accordionSolution2">
 <div class="accordion-body">
 <p>TODO</p>
 </div>
@@ -862,8 +862,8 @@ <h1 id="further-reading">Further Reading<a class="anchor" aria-label="anchor" hr
   "url": "https://ccb-hms.github.io/osca-carpentries/intro-sce.html",
   "identifier": "https://ccb-hms.github.io/osca-carpentries/intro-sce.html",
   "dateCreated": "2024-01-10",
-  "dateModified": "2024-03-25",
-  "datePublished": "2024-04-02"
+  "dateModified": "2024-04-09",
+  "datePublished": "2024-04-09"
 }
 
   </script><script>
diff --git a/key-points.html b/key-points.html
index 27ebc76..5ecec29 100644
--- a/key-points.html
+++ b/key-points.html
@@ -383,8 +383,8 @@ <h2 class="accordion-header" id="flush-headingTwelve">
   "url": "https://ccb-hms.github.io/osca-carpentries/key-points.html",
   "identifier": "https://ccb-hms.github.io/osca-carpentries/key-points.html",
   "dateCreated": "2024-01-10",
-  "dateModified": "2024-04-02",
-  "datePublished": "2024-04-02"
+  "dateModified": "2024-04-09",
+  "datePublished": "2024-04-09"
 }
 
   </script><script>
diff --git a/large_data.html b/large_data.html
index 37916ea..e0f65c3 100644
--- a/large_data.html
+++ b/large_data.html
@@ -260,7 +260,7 @@ <h2 class="accordion-header" id="flush-headingTwelve">
       </div>
       <hr></nav><main id="main-content" class="main-content"><div class="container lesson-content">
         <h1>Working with large data</h1>
-        <p>Last updated on 2024-03-25 |
+        <p>Last updated on 2024-04-09 |
         
         <a href="https://github.com/ccb-hms/osca-carpentries/edit/main/episodes/large_data.Rmd" class="external-link">Edit this page <i aria-hidden="true" data-feather="edit"></i></a></p>
         
@@ -722,24 +722,20 @@ <h3 class="code-label">OUTPUT<i aria-hidden="true" data-feather="chevron-left"><
 <pre class="output" tabindex="0"><code>     approx
 exact   1   2   3   4   5   6   7   8   9  10  11  12  13  14  15
    1   90   0   0   0   0   0   0   0   1   0   0   0   0   0   0
-   2    0 143   0   1   0   0   0   0   0   0   0   0   0   0   0
+   2    0 143   0   0   0   0   0   0   0   0   0   0   0   0   1
    3    0   0  75   0   0   0   0   0   0   0   0   0   0   0   0
-   4    0   0   0 253   0   0   0   0   0   0   0   0 144   0   0
-   5    0   0   0   0  71   0   0  95   0   0   0   5   0   0   0
-   6    0   0   0   0   0 210   0   0   0   0   0   0   0   0   0
-   7    0   0   0   0   0   1 244   0   0   1   0   0   0   0   0
-   8    1   0   0   0   1   0   0   0 106   0   0   0   0   0   0
-   9    0   0   0   0   0   0   0   0   0 112   0  10   0   0   0
-   10   0   0   0   0   0   1   0   0   0   1 145   0   0   6   0
-   11   0   0   0   0   3   0   0   0   0   0   0 197   0   0   0
-   12   0   0   2   0 324   0   0   0   0   1   0   0   0   0   0
-   13   0   0   0   0   0   0   0   0   0   1   0   0   0 146   0
-   14   0   0   0   0   0   0   0   0   0   0   0   0   0   0  20</code></pre>
-</div>
-<div class="codewrapper">
-<h3 class="code-label">ERROR<i aria-hidden="true" data-feather="chevron-left"></i><i aria-hidden="true" data-feather="chevron-right"></i>
-</h3>
-<pre class="error" tabindex="0"><code>Error: rand &gt; 0.85 is not TRUE</code></pre>
+   4    0   0   0 341   0   0   0   0   0   0   0   0   0   0   0
+   5    1   0   0   0 392   0   0   0   1   1   0   3   0   0   0
+   6    0   0   0   0   0 209   0   0   0   0   0   0   0   0   0
+   7    0   0   0   0   0   0 245   0   0   1   0   0   0   0   0
+   8    0   0   0   0   1   0   0  95   0   0   0   0   0   0   0
+   9    1   0   0   0   1   0   0   0 106   0   0   0   0   0   0
+   10   0   0   0   0   0   0   0   0   0 112   4   0   1   0   0
+   11   0   0   0   0   0   1   0   0   0   0 143   0   0   0   0
+   12   0   0   0   0   3   0   0   0   0  10   0 201   0   0   0
+   13   0   0   0   0   0   0   0   0   0   0   6   0 146   0   0
+   14   0   0   0   0   0   0   0   0   0   0   0   0   0  20   0
+   15   0   0   0   0   0   0   0   0   0   0   0   0   0   0  56</code></pre>
 </div>
 <p>Note that Annoy writes the NN index to disk prior to performing the
 search. Thus, it may not actually be faster than the default exact
@@ -748,7 +744,7 @@ <h3 class="code-label">ERROR<i aria-hidden="true" data-feather="chevron-left"></
 also not difficult to find situations where the approximation
 deteriorates, especially at high dimensions, though this may not have an
 appreciable impact on the biological conclusions.</p>
-<div class="codewrapper sourceCode" id="cb30">
+<div class="codewrapper sourceCode" id="cb29">
 <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i aria-hidden="true" data-feather="chevron-right"></i>
 </h3>
 <pre class="downlit sourceCode r">
@@ -782,7 +778,7 @@ <h2 id="singular-value-decomposition">Singular value decomposition<a class="anch
 change the SVD algorithm used in any of these functions by simply
 specifying an alternative value for the <code>BSPARAM</code>
 argument.</p>
-<div class="codewrapper sourceCode" id="cb32">
+<div class="codewrapper sourceCode" id="cb31">
 <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i aria-hidden="true" data-feather="chevron-right"></i>
 </h3>
 <pre class="downlit sourceCode r">
@@ -808,7 +804,7 @@ <h3 class="code-label">OUTPUT<i aria-hidden="true" data-feather="chevron-left"><
   .. ..$ : chr [1:500] "ENSMUSG00000055609" "ENSMUSG00000052217" "ENSMUSG00000069919" "ENSMUSG00000048583" ...
   .. ..$ : chr [1:20] "PC1" "PC2" "PC3" "PC4" ...</code></pre>
 </div>
-<div class="codewrapper sourceCode" id="cb34">
+<div class="codewrapper sourceCode" id="cb33">
 <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i aria-hidden="true" data-feather="chevron-right"></i>
 </h3>
 <pre class="downlit sourceCode r">
@@ -855,7 +851,7 @@ <h2 id="seurat">Seurat<a class="anchor" aria-label="anchor" href="#seurat"></a><
 types of single-cell data. Seurat is developed and maintained by the <a href="https://satijalab.org/seurat/authors.html" class="external-link">Satija lab</a> and is
 released under the <a href="https://opensource.org/license/mit/" class="external-link">MIT
 license</a>.</p>
-<div class="codewrapper sourceCode" id="cb36">
+<div class="codewrapper sourceCode" id="cb35">
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 </h3>
 <pre class="downlit sourceCode r">
@@ -877,7 +873,7 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 with functionality from OSCA/Bioconductor. We therefore need to first
 install the <a href="https://github.com/satijalab/seurat-data" class="external-link">SeuratData</a> package,
 which is available from GitHub only.</p>
-<div class="codewrapper sourceCode" id="cb37">
+<div class="codewrapper sourceCode" id="cb36">
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 </h3>
 <pre class="downlit sourceCode r">
@@ -886,7 +882,7 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 </div>
 <p>We then proceed by loading all required packages and installing the
 PBMC dataset:</p>
-<div class="codewrapper sourceCode" id="cb38">
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 <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i aria-hidden="true" data-feather="chevron-right"></i>
 </h3>
 <pre class="downlit sourceCode r">
@@ -895,7 +891,7 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 </div>
 <p>We then load the dataset as an <code>SeuratObject</code> and convert
 it to a <code>SingleCellExperiment</code>.</p>
-<div class="codewrapper sourceCode" id="cb39">
+<div class="codewrapper sourceCode" id="cb38">
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 </h3>
 <pre class="downlit sourceCode r">
@@ -909,7 +905,7 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 <p>Seurat also allows conversion from <code>SingleCellExperiment</code>
 objects to Seurat objects; we demonstrate this here on the wild-type
 chimera mouse gastrulation dataset.</p>
-<div class="codewrapper sourceCode" id="cb40">
+<div class="codewrapper sourceCode" id="cb39">
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 </h3>
 <pre class="downlit sourceCode r">
@@ -920,7 +916,7 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 </div>
 <p>After some processing of the dataset, the actual conversion is
 carried out with the <code>as.Seurat</code> function.</p>
-<div class="codewrapper sourceCode" id="cb41">
+<div class="codewrapper sourceCode" id="cb40">
 <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i aria-hidden="true" data-feather="chevron-right"></i>
 </h3>
 <pre class="downlit sourceCode r">
@@ -952,7 +948,7 @@ <h2 id="scanpy">Scanpy<a class="anchor" aria-label="anchor" href="#scanpy"></a><
 to enable users and developers to easily move data between these
 frameworks to construct a multi-language analysis pipeline across
 R/Bioconductor and Python.</p>
-<div class="codewrapper sourceCode" id="cb42">
+<div class="codewrapper sourceCode" id="cb41">
 <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i aria-hidden="true" data-feather="chevron-right"></i>
 </h3>
 <pre class="downlit sourceCode r">
@@ -962,7 +958,7 @@ <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i
 <code>SingleCellExperiment</code> from an H5AD file. Here, we use an
 example H5AD file contained in the <em><a href="https://bioconductor.org/packages/3.18/zellkonverter" class="external-link">zellkonverter</a></em>
 package.</p>
-<div class="codewrapper sourceCode" id="cb43">
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 </h3>
 <pre class="downlit sourceCode r">
@@ -1010,7 +1006,7 @@ <h3 class="code-label">OUTPUT<i aria-hidden="true" data-feather="chevron-left"><
 <p>We can also write a <code>SingleCellExperiment</code> to an H5AD file
 with the <code>writeH5AD()</code> function. This is demonstrated below
 on the wild-type chimera mouse gastrulation dataset.</p>
-<div class="codewrapper sourceCode" id="cb49">
+<div class="codewrapper sourceCode" id="cb48">
 <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i aria-hidden="true" data-feather="chevron-right"></i>
 </h3>
 <pre class="downlit sourceCode r">
@@ -1030,7 +1026,7 @@ <h3 class="code-label">OUTPUT<i aria-hidden="true" data-feather="chevron-left"><
 </div>
 <div class="section level1">
 <h1 id="session-info">Session Info<a class="anchor" aria-label="anchor" href="#session-info"></a></h1>
-<div class="codewrapper sourceCode" id="cb51">
+<div class="codewrapper sourceCode" id="cb50">
 <h3 class="code-label">R<i aria-hidden="true" data-feather="chevron-left"></i><i aria-hidden="true" data-feather="chevron-right"></i>
 </h3>
 <pre class="downlit sourceCode r">
@@ -1189,7 +1185,7 @@ <h3 class="callout-title">Exercise 1: Out of memory representation<a class="anch
 <button class="accordion-button hint-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseHint1" aria-expanded="false" aria-controls="collapseHint1">
   <h4 class="accordion-header" id="headingHint1">Give me a hint</h4>
 </button>
-<div id="collapseHint1" class="accordion-collapse collapse" aria-labelledby="headingHint1" data-bs-parent="#accordionHint1">
+<div id="collapseHint1" class="accordion-collapse collapse" data-bs-parent="#accordionHint1" aria-labelledby="headingHint1">
 <div class="accordion-body">
 <p>See the <code>HDF5Array</code> function for reading from HDF5 and the
 <code>writeHDF5Array</code> function for writing to HDF5 from the <em><a href="https://bioconductor.org/packages/3.18/HDF5Array" class="external-link">HDF5Array</a></em>
@@ -1203,7 +1199,7 @@ <h4 class="accordion-header" id="headingHint1">Give me a hint</h4>
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 <div class="accordion-body">
 <p>TODO</p>
 </div>
@@ -1231,7 +1227,7 @@ <h3 class="callout-title">Exercise 2: Parallelization<a class="anchor" aria-labe
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   <h4 class="accordion-header" id="headingHint2">Give me a hint</h4>
 </button>
-<div id="collapseHint2" class="accordion-collapse collapse" aria-labelledby="headingHint2" data-bs-parent="#accordionHint2">
+<div id="collapseHint2" class="accordion-collapse collapse" data-bs-parent="#accordionHint2" aria-labelledby="headingHint2">
 <div class="accordion-body">
 <p>Use the function <code>system.time</code> to obtain the runtime of
 each job.</p>
@@ -1244,7 +1240,7 @@ <h4 class="accordion-header" id="headingHint2">Give me a hint</h4>
 <button class="accordion-button solution-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseSolution2" aria-expanded="false" aria-controls="collapseSolution2">
   <h4 class="accordion-header" id="headingSolution2">Show me the solution</h4>
 </button>
-<div id="collapseSolution2" class="accordion-collapse collapse" aria-labelledby="headingSolution2" data-bs-parent="#accordionSolution2">
+<div id="collapseSolution2" class="accordion-collapse collapse" data-bs-parent="#accordionSolution2" aria-labelledby="headingSolution2">
 <div class="accordion-body">
 <p>TODO</p>
 </div>
@@ -1274,7 +1270,7 @@ <h3 class="callout-title">Exercise 3: Conversion to Seurat<a class="anchor" aria
 <button class="accordion-button hint-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseHint3" aria-expanded="false" aria-controls="collapseHint3">
   <h4 class="accordion-header" id="headingHint3">Give me a hint</h4>
 </button>
-<div id="collapseHint3" class="accordion-collapse collapse" aria-labelledby="headingHint3" data-bs-parent="#accordionHint3">
+<div id="collapseHint3" class="accordion-collapse collapse" data-bs-parent="#accordionHint3" aria-labelledby="headingHint3">
 <div class="accordion-body">
 <p>Use Seurat’s <code>DimPlot</code> function.</p>
 </div>
@@ -1286,7 +1282,7 @@ <h4 class="accordion-header" id="headingHint3">Give me a hint</h4>
 <button class="accordion-button solution-button collapsed" type="button" data-bs-toggle="collapse" data-bs-target="#collapseSolution3" aria-expanded="false" aria-controls="collapseSolution3">
   <h4 class="accordion-header" id="headingSolution3">Show me the solution</h4>
 </button>
-<div id="collapseSolution3" class="accordion-collapse collapse" aria-labelledby="headingSolution3" data-bs-parent="#accordionSolution3">
+<div id="collapseSolution3" class="accordion-collapse collapse" data-bs-parent="#accordionSolution3" aria-labelledby="headingSolution3">
 <div class="accordion-body">
 <p>Use Seurat’s <code>DimPlot</code> function.</p>
 </div>
@@ -1374,8 +1370,8 @@ <h1 id="references">References<a class="anchor" aria-label="anchor" href="#refer
   "url": "https://ccb-hms.github.io/osca-carpentries/large_data.html",
   "identifier": "https://ccb-hms.github.io/osca-carpentries/large_data.html",
   "dateCreated": "2024-01-10",
-  "dateModified": "2024-03-25",
-  "datePublished": "2024-04-02"
+  "dateModified": "2024-04-09",
+  "datePublished": "2024-04-09"
 }
 
   </script><script>
diff --git a/md5sum.txt b/md5sum.txt
index 1230d86..5e90699 100644
--- a/md5sum.txt
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@@ -1,17 +1,17 @@
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diff --git a/multi-sample.html b/multi-sample.html
index 12cf36d..f74c327 100644
--- a/multi-sample.html
+++ b/multi-sample.html
@@ -260,7 +260,7 @@ <h2 class="accordion-header" id="flush-headingTwelve">
       </div>
       <hr></nav><main id="main-content" class="main-content"><div class="container lesson-content">
         <h1>Multi-sample analyses</h1>
-        <p>Last updated on 2024-03-25 |
+        <p>Last updated on 2024-04-09 |
         
         <a href="https://github.com/ccb-hms/osca-carpentries/edit/main/episodes/multi-sample.Rmd" class="external-link">Edit this page <i aria-hidden="true" data-feather="edit"></i></a></p>
         
@@ -1374,8 +1374,8 @@ <h3 class="callout-title">Key Points<a class="anchor" aria-label="anchor" href="
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   "identifier": "https://ccb-hms.github.io/osca-carpentries/multi-sample.html",
   "dateCreated": "2024-01-10",
-  "dateModified": "2024-03-25",
-  "datePublished": "2024-04-02"
+  "dateModified": "2024-04-09",
+  "datePublished": "2024-04-09"
 }
 
   </script><script>
diff --git a/pkgdown.yml b/pkgdown.yml
index 67524b8..b605228 100644
--- a/pkgdown.yml
+++ b/pkgdown.yml
@@ -2,5 +2,5 @@ pandoc: 3.1.11
 pkgdown: 2.0.7
 pkgdown_sha: ~
 articles: {}
-last_built: 2024-04-02T00:58Z
+last_built: 2024-04-09T01:13Z
 
diff --git a/profiles.html b/profiles.html
index 122a751..75a6dba 100644
--- a/profiles.html
+++ b/profiles.html
@@ -293,8 +293,8 @@ <h1>Learner Profiles</h1>
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   "identifier": "https://ccb-hms.github.io/osca-carpentries/profiles.html",
   "dateCreated": "2024-01-10",
-  "dateModified": "2024-04-02",
-  "datePublished": "2024-04-02"
+  "dateModified": "2024-04-09",
+  "datePublished": "2024-04-09"
 }
 
   </script><script>
diff --git a/reference.html b/reference.html
index 8ccacbd..697c302 100644
--- a/reference.html
+++ b/reference.html
@@ -256,7 +256,7 @@ <h2 class="accordion-header" id="flush-headingTwelve">
       </div>
       <hr></nav><main id="main-content" class="main-content"><div class="container lesson-content">
         <h1>Reference</h1>
-        <p>Last updated on 2024-03-25 |
+        <p>Last updated on 2024-04-09 |
         
         <a href="https://github.com/ccb-hms/osca-carpentries/edit/main/learners/reference.md" class="external-link">Edit this page <i aria-hidden="true" data-feather="edit"></i></a></p>
         
@@ -332,8 +332,8 @@ <h1>Reference</h1>
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   "identifier": "https://ccb-hms.github.io/osca-carpentries/reference.html",
   "dateCreated": "2024-01-10",
-  "dateModified": "2024-03-25",
-  "datePublished": "2024-04-02"
+  "dateModified": "2024-04-09",
+  "datePublished": "2024-04-09"
 }
 
   </script><script>