scaffold_and_refine_multitaxa -- more thorough assembly outputs

.dockstore.yml

@@ -374,6 +374,11 @@ workflows:
     primaryDescriptorPath: /pipes/WDL/workflows/terra_table_to_tsv.wdl
     testParameterFiles:
       - /empty.json
+  - name: terra_tsv_to_table
+    subclass: WDL
+    primaryDescriptorPath: /pipes/WDL/workflows/terra_tsv_to_table.wdl
+    testParameterFiles:
+      - /empty.json
   - name: terra_update_assemblies
     subclass: WDL
     primaryDescriptorPath: /pipes/WDL/workflows/terra_update_assemblies.wdl
@@ -394,13 +399,11 @@ workflows:
     primaryDescriptorPath: /pipes/WDL/workflows/bam_to_qiime.wdl
     testParameterFiles:
       - /empty.json
-
   - name: create_enterics_qc_viz
     subclass: WDL
     primaryDescriptorPath: /pipes/WDL/workflows/create_enterics_qc_viz.wdl
     testParameterFiles:
       - /empty.json
-
   - name: create_enterics_qc_viz_general
     subclass: WDL
     primaryDescriptorPath: /pipes/WDL/workflows/create_enterics_qc_viz_general.wdl

pipes/WDL/tasks/tasks_assembly.wdl

@@ -578,13 +578,14 @@ task refine_assembly_with_aligned_reads {
     input {
       File     reference_fasta
       File     reads_aligned_bam
-      String   sample_name
+      String   out_basename = basename(reads_aligned_bam, '.bam')
+      String   sample_name = out_basename
 
       Boolean  mark_duplicates = false
       Float    major_cutoff = 0.5
       Int      min_coverage = 3
 
-      Int?     machine_mem_gb
+      Int      machine_mem_gb = 15
       String   docker = "quay.io/broadinstitute/viral-assemble:2.2.4.0"
     }
 
@@ -614,7 +615,7 @@ task refine_assembly_with_aligned_reads {
       }
     }
 
-    command {
+    command <<<
         set -ex -o pipefail
 
         # find 90% memory
@@ -639,36 +640,36 @@ task refine_assembly_with_aligned_reads {
           temp_markdup.bam \
           refined.fasta \
           --already_realigned_bam=temp_markdup.bam \
-          --outVcf ~{sample_name}.sites.vcf.gz \
+          --outVcf "~{out_basename}.sites.vcf.gz" \
           --min_coverage ~{min_coverage} \
           --major_cutoff ~{major_cutoff} \
           --JVMmemory "$mem_in_mb"m \
           --loglevel=DEBUG
 
         file_utils.py rename_fasta_sequences \
-          refined.fasta "${sample_name}.fasta" "${sample_name}"
+          refined.fasta "~{out_basename}.fasta" "~{sample_name}"
 
         # collect variant counts
         if (( $(cat refined.fasta | wc -l) > 1 )); then
-          bcftools filter -e "FMT/DP<${min_coverage}" -S . "${sample_name}.sites.vcf.gz" -Ou | bcftools filter -i "AC>1" -Ou > "${sample_name}.diffs.vcf"
-          bcftools filter -i 'TYPE="snp"'  "${sample_name}.diffs.vcf" | bcftools query -f '%POS\n' | wc -l | tee num_snps
-          bcftools filter -i 'TYPE!="snp"' "${sample_name}.diffs.vcf" | bcftools query -f '%POS\n' | wc -l | tee num_indels
+          bcftools filter -e "FMT/DP<~{min_coverage}" -S . "~{out_basename}.sites.vcf.gz" -Ou | bcftools filter -i "AC>1" -Ou > "~{out_basename}.diffs.vcf"
+          bcftools filter -i 'TYPE="snp"'  "~{out_basename}.diffs.vcf" | bcftools query -f '%POS\n' | wc -l | tee num_snps
+          bcftools filter -i 'TYPE!="snp"' "~{out_basename}.diffs.vcf" | bcftools query -f '%POS\n' | wc -l | tee num_indels
         else
           # empty output
           echo "0" > num_snps
           echo "0" > num_indels
-          cp "${sample_name}.sites.vcf.gz" "${sample_name}.diffs.vcf"
+          cp "~{out_basename}.sites.vcf.gz" "~{out_basename}.diffs.vcf"
         fi
 
         # collect figures of merit
         set +o pipefail # grep will exit 1 if it fails to find the pattern
         grep -v '^>' refined.fasta | tr -d '\n' | wc -c | tee assembly_length
         grep -v '^>' refined.fasta | tr -d '\nNn' | wc -c | tee assembly_length_unambiguous
-    }
+    >>>
 
     output {
-        File   refined_assembly_fasta      = "${sample_name}.fasta"
-        File   sites_vcf_gz                = "${sample_name}.sites.vcf.gz"
+        File   refined_assembly_fasta      = "~{out_basename}.fasta"
+        File   sites_vcf_gz                = "~{out_basename}.sites.vcf.gz"
         Int    assembly_length             = read_int("assembly_length")
         Int    assembly_length_unambiguous = read_int("assembly_length_unambiguous")
         Int    dist_to_ref_snps            = read_int("num_snps")
@@ -678,7 +679,7 @@ task refine_assembly_with_aligned_reads {
 
     runtime {
         docker: docker
-        memory: select_first([machine_mem_gb, 15]) + " GB"
+        memory: machine_mem_gb + " GB"
         cpu: 8
         disks:  "local-disk " + disk_size + " LOCAL"
         disk: disk_size + " GB" # TES

pipes/WDL/tasks/tasks_nextstrain.wdl

@@ -1147,6 +1147,8 @@ task augur_mafft_align {
 
         String  docker = "nextstrain/base:build-20230905T192825Z"
         Int     disk_size = 750
+        Int     mem_size = 180
+        Int     cpus = 64
     }
     command <<<
         set -e
@@ -1165,8 +1167,8 @@ task augur_mafft_align {
     >>>
     runtime {
         docker: docker
-        memory: "180 GB"
-        cpu :   64
+        memory: mem_size + " GB"
+        cpu :   cpus
         disks:  "local-disk " + disk_size + " LOCAL"
         disk: disk_size + " GB" # TES
         preemptible: 0

pipes/WDL/workflows/assemble_refbased.wdl

@@ -63,6 +63,7 @@ workflow assemble_refbased {
         Array[File]+ reads_unmapped_bams
         File         reference_fasta
         String       sample_name = basename(reads_unmapped_bams[0], '.bam')
+        String?      sample_original_name
 
         String       aligner="minimap2"
         File?        novocraft_license
@@ -150,7 +151,8 @@ workflow assemble_refbased {
             reads_aligned_bam = aligned_trimmed_bam,
             min_coverage      = min_coverage,
             major_cutoff      = major_cutoff,
-            sample_name       = sample_name
+            out_basename      = sample_name,
+            sample_name       = select_first([sample_original_name, sample_name])
     }
 
     scatter(reads_unmapped_bam in reads_unmapped_bams) {

pipes/WDL/workflows/scaffold_and_refine_multitaxa.wdl

@@ -3,6 +3,7 @@ version 1.0
 import "../tasks/tasks_assembly.wdl" as assembly
 import "../tasks/tasks_metagenomics.wdl" as metagenomics
 import "../tasks/tasks_ncbi.wdl" as ncbi
+import "../tasks/tasks_reports.wdl" as reports
 import "../tasks/tasks_utils.wdl" as utils
 import "assemble_refbased.wdl" as assemble_refbased
 
@@ -16,6 +17,7 @@ workflow scaffold_and_refine_multitaxa {
 
     input {
         String  sample_id
+        Array[String] sample_names
         File    reads_unmapped_bam
 
         File    taxid_to_ref_accessions_tsv
@@ -25,7 +27,8 @@ workflow scaffold_and_refine_multitaxa {
         # Float    min_pct_reference_covered = 0.1
     }
 
-    Int  min_scaffold_unambig = 10
+    Int    min_scaffold_unambig = 10
+    String sample_original_name = flatten([sample_names, [sample_id]])[0]
 
     # if kraken reports are available, filter scaffold list to observed hits (output might be empty!)
     if(defined(focal_report_tsv) && defined(ncbi_taxdump_tgz)) {
@@ -37,7 +40,7 @@ workflow scaffold_and_refine_multitaxa {
         }
     }
 
-    Array[String] assembly_header = ["sample_id", "taxid", "tax_name", "assembly_fasta", "aligned_only_reads_bam", "coverage_plot", "assembly_length", "assembly_length_unambiguous", "reads_aligned", "mean_coverage", "percent_reference_covered", "intermediate_gapfill_fasta", "assembly_preimpute_length_unambiguous", "replicate_concordant_sites", "replicate_discordant_snps", "replicate_discordant_indels", "replicate_discordant_vcf", "isnvsFile", "aligned_bam", "coverage_tsv", "read_pairs_aligned", "bases_aligned"]
+    Array[String] assembly_header = ["entity:assembly_id", "assembly_name", "sample_id", "sample_name", "taxid", "tax_name", "assembly_fasta", "aligned_only_reads_bam", "coverage_plot", "assembly_length", "assembly_length_unambiguous", "reads_aligned", "mean_coverage", "percent_reference_covered", "scaffolding_num_segments_recovered", "reference_num_segments_required", "reference_length", "reference_accessions", "intermediate_gapfill_fasta", "assembly_preimpute_length_unambiguous", "replicate_concordant_sites", "replicate_discordant_snps", "replicate_discordant_indels", "replicate_discordant_vcf", "isnvsFile", "aligned_bam", "coverage_tsv", "read_pairs_aligned", "bases_aligned", "coverage_genbank", "assembly_method", "sample"]
     Array[Array[String]] taxid_to_ref_accessions = read_tsv(select_first([filter_refs_to_found_taxa.filtered_taxid_to_ref_accessions_tsv, taxid_to_ref_accessions_tsv]))
     scatter(taxon in taxid_to_ref_accessions) {
         # cromwell's read_tsv emits [[""]] on empty (0-byte) file input, turn it into []
@@ -70,35 +73,51 @@ workflow scaffold_and_refine_multitaxa {
             # polish de novo assembly with reads
             call assemble_refbased.assemble_refbased as refine {
                 input:
-                    reads_unmapped_bams = [reads_unmapped_bam],
-                    reference_fasta     = scaffold.scaffold_fasta,
-                    sample_name         = sample_id
+                    reads_unmapped_bams  = [reads_unmapped_bam],
+                    reference_fasta      = scaffold.scaffold_fasta,
+                    sample_name          = sample_id,
+                    sample_original_name = sample_original_name
             }
             String assembly_method_denovo = "viral-ngs/assemble_denovo"
         }
         if (scaffold.assembly_preimpute_length_unambiguous <= min_scaffold_unambig) {
             # fall back to refbased assembly if de novo fails
             call assemble_refbased.assemble_refbased as ref_based {
                 input:
-                    reads_unmapped_bams = [reads_unmapped_bam],
-                    reference_fasta     = download_annotations.combined_fasta,
-                    sample_name         = sample_id
+                    reads_unmapped_bams  = [reads_unmapped_bam],
+                    reference_fasta      = download_annotations.combined_fasta,
+                    sample_name          = sample_id,
+                    sample_original_name = sample_original_name
             }
             String assembly_method_refbased = "viral-ngs/assemble_refbased"
         }
 
-        # TO DO: if percent_reference_covered > some threshold, run ncbi.rename_fasta_header and ncbi.align_and_annot_transfer_single
-        # TO DO: if biosample attributes file provided, run ncbi.biosample_to_genbank
-
-        String taxid = taxon[0]
-        String tax_name = taxon[1]
         Int    assembly_length_unambiguous = select_first([refine.assembly_length_unambiguous, ref_based.assembly_length_unambiguous])
         Float  percent_reference_covered = 1.0 * assembly_length_unambiguous / scaffold.reference_length
         File   assembly_fasta = select_first([refine.assembly_fasta, ref_based.assembly_fasta])
+
+        if(assembly_length_unambiguous > 0) {
+            call reports.coverage_report as coverage_self {
+                input:
+                    mapped_bams = select_all([refine.align_to_self_merged_aligned_only_bam, ref_based.align_to_self_merged_aligned_only_bam]),
+                    mapped_bam_idx = []
+            }
+            call utils.tsv_drop_cols as coverage_two_col {
+                input:
+                    in_tsv = coverage_self.coverage_report,
+                    drop_cols = ["aln2self_cov_median", "aln2self_cov_mean_non0", "aln2self_cov_1X", "aln2self_cov_5X", "aln2self_cov_20X", "aln2self_cov_100X"]
+            }
+        }
+
+        String taxid = taxon[0]
+        String tax_name = taxon[1]
         Map[String, String] stats_by_taxon = {
-            "sample_id" : sample_id,
-            "taxid" : taxid,
-            "tax_name" : tax_name,
+            "entity:assembly_id" : sample_id + "-" + taxid,
+            "assembly_name" :      tax_name + ": " + sample_original_name,
+            "sample_id" :          sample_id,
+            "sample_name" :        sample_original_name,
+            "taxid" :              taxid,
+            "tax_name" :           tax_name,
 
             "assembly_fasta" :              assembly_fasta,
             "aligned_only_reads_bam" :      select_first([refine.align_to_self_merged_aligned_only_bam, ref_based.align_to_self_merged_aligned_only_bam]),
@@ -108,6 +127,10 @@ workflow scaffold_and_refine_multitaxa {
             "reads_aligned" :               select_first([refine.align_to_self_merged_reads_aligned, ref_based.align_to_self_merged_reads_aligned]),
             "mean_coverage" :               select_first([refine.align_to_self_merged_mean_coverage, ref_based.align_to_self_merged_mean_coverage]),
             "percent_reference_covered" :   percent_reference_covered,
+            "scaffolding_num_segments_recovered" : scaffold.assembly_num_segments_recovered,
+            "reference_num_segments_required" : scaffold.reference_num_segments_required,
+            "reference_length" :            scaffold.reference_length,
+            "reference_accessions" :        taxon[2],
 
             "intermediate_gapfill_fasta" :            scaffold.intermediate_gapfill_fasta,
             "assembly_preimpute_length_unambiguous" : scaffold.assembly_preimpute_length_unambiguous,
@@ -122,15 +145,18 @@ workflow scaffold_and_refine_multitaxa {
             "coverage_tsv" :       select_first([refine.align_to_self_merged_coverage_tsv, ref_based.align_to_self_merged_coverage_tsv]),
             "read_pairs_aligned" : select_first([refine.align_to_self_merged_read_pairs_aligned, ref_based.align_to_self_merged_read_pairs_aligned]),
             "bases_aligned" :      select_first([refine.align_to_self_merged_bases_aligned, ref_based.align_to_self_merged_bases_aligned]),
+            "coverage_genbank" :   select_first([coverage_two_col.out_tsv, ""]),
+
+            "assembly_method" :    select_first([assembly_method_denovo, assembly_method_refbased]),
 
-            "assembly_method" :    select_first([assembly_method_denovo, assembly_method_refbased])
+            "sample":              '{"entityType":"sample","entityName":"' + sample_id + '"}'
         }
 
         scatter(h in assembly_header) {
             String stat_by_taxon = stats_by_taxon[h]
         }
     }
- 
+
     ### summary stats
     call utils.concatenate {
       input:

pipes/WDL/workflows/terra_tsv_to_table.wdl

@@ -0,0 +1,21 @@
+version 1.0
+
+#DX_SKIP_WORKFLOW
+
+import "../tasks/tasks_terra.wdl" as terra
+
+workflow terra_tsv_to_table {
+    meta {
+        description: "Upload tsv file to Terra data table: insert-or-update on existing rows/columns"
+        author: "Broad Viral Genomics"
+        email:  "[email protected]"
+    }
+
+    call terra.check_terra_env
+
+    call terra.upload_entities_tsv {
+        input:
+            workspace_name   = check_terra_env.workspace_name,
+            terra_project    = check_terra_env.workspace_namespace
+    }
+}