-
Notifications
You must be signed in to change notification settings - Fork 27
New issue
Have a question about this project? Sign up for a free GitHub account to open an issue and contact its maintainers and the community.
By clicking “Sign up for GitHub”, you agree to our terms of service and privacy statement. We’ll occasionally send you account related emails.
Already on GitHub? Sign in to your account
streamline NCBI submissions #191
Merged
Merged
Conversation
This file contains bidirectional Unicode text that may be interpreted or compiled differently than what appears below. To review, open the file in an editor that reveals hidden Unicode characters.
Learn more about bidirectional Unicode characters
…d in workflow yet)
…nbanks source modifier table
…post metadata merge in subsample_by_metadata_with_focal
…evel options properly
Closed
Sign up for free
to join this conversation on GitHub.
Already have an account?
Sign in to comment
Add this suggestion to a batch that can be applied as a single commit.
This suggestion is invalid because no changes were made to the code.
Suggestions cannot be applied while the pull request is closed.
Suggestions cannot be applied while viewing a subset of changes.
Only one suggestion per line can be applied in a batch.
Add this suggestion to a batch that can be applied as a single commit.
Applying suggestions on deleted lines is not supported.
You must change the existing code in this line in order to create a valid suggestion.
Outdated suggestions cannot be applied.
This suggestion has been applied or marked resolved.
Suggestions cannot be applied from pending reviews.
Suggestions cannot be applied on multi-line comments.
Suggestions cannot be applied while the pull request is queued to merge.
Suggestion cannot be applied right now. Please check back later.
adds sarscov2_genbank workflow, which preps assemblies for submission to genbank using their new process for SARS-CoV-2 (use the previous workflows for other taxa--though my guess is that genbank will roll these changes out across taxa eventually)
adds the VADR (Viral Annotation DefineR) tool, written by genbank, to produce tbl files on assemblies and pre-screen submissions to those that are expected to pass QC on NCBI's end
adds sra_meta_prep task and the new demux_deplete workflow, which runs demux on all lanes of a flowcell, runs human read depletion, and packages cleaned reads for SRA submission (requires a more verbose custom samplesheet than the default Illumina sheets and requires pre-registered NCBI BioSamples, but this should be used for all high throughput sequencing projects)
some preliminary work on an end-to-end SARS-CoV-2 pipeline (sarscov2_illumina_full), but it is not quite ready yet
bump pangolin and nextclade docker images
incorporate metadata tsv merging to augur_from_msa
replace augur_from_assemblies to incorporate tsv merging, derivedcols, subsample_by_metadata_with_focal and augur_from_msa, turning it into an end-to-end pipeline from global database download, merged in with custom data, to new nextstrain builds (takes a while though)