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streamline NCBI submissions #191

Merged
merged 55 commits into from
Jan 12, 2021
Merged

streamline NCBI submissions #191

merged 55 commits into from
Jan 12, 2021

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dpark01
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@dpark01 dpark01 commented Jan 12, 2021

  • adds sarscov2_genbank workflow, which preps assemblies for submission to genbank using their new process for SARS-CoV-2 (use the previous workflows for other taxa--though my guess is that genbank will roll these changes out across taxa eventually)

  • adds the VADR (Viral Annotation DefineR) tool, written by genbank, to produce tbl files on assemblies and pre-screen submissions to those that are expected to pass QC on NCBI's end

  • adds sra_meta_prep task and the new demux_deplete workflow, which runs demux on all lanes of a flowcell, runs human read depletion, and packages cleaned reads for SRA submission (requires a more verbose custom samplesheet than the default Illumina sheets and requires pre-registered NCBI BioSamples, but this should be used for all high throughput sequencing projects)

  • some preliminary work on an end-to-end SARS-CoV-2 pipeline (sarscov2_illumina_full), but it is not quite ready yet

  • bump pangolin and nextclade docker images

  • incorporate metadata tsv merging to augur_from_msa

  • replace augur_from_assemblies to incorporate tsv merging, derivedcols, subsample_by_metadata_with_focal and augur_from_msa, turning it into an end-to-end pipeline from global database download, merged in with custom data, to new nextstrain builds (takes a while though)

dpark01 added 30 commits January 5, 2021 20:11
…post metadata merge in subsample_by_metadata_with_focal
@dpark01 dpark01 merged commit 0d5a361 into master Jan 12, 2021
@dpark01 dpark01 mentioned this pull request Jan 26, 2021
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