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Bug: white space in the 'QNAME' field in bam-input-file causes invalid but completed alignment #2591

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lutrarutra opened this issue Feb 13, 2025 · 0 comments

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@lutrarutra
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STAR version 2.7.11b

Given input A00XXX:XXX:XXX:XXX:1000 1:N:0:YYY+YYY, STAR aligner assumes wrong column (most likely explanation) as the SEQ resulting in 0 aligned reads.

In the output log STAR reports: Average input read length 1

No exception is raised.

Relevant input arguments:

    --readFilesType SAM SE \
    --readFilesCommand "samtools view -f 4" 

I know there is not supposed to be a white space in the QNAME, but in some cases there still is, perhaps copied from the fastq.gz by some hacky script/software.

Maybe STAR aligner could check that there is no white-space, and if there is, raise warning/error or temporarily fix the QNAME?

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