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How to create both Gene count and Trascriptome count for RSEM from Bam file? #2589

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lida-R opened this issue Feb 10, 2025 · 0 comments
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@lida-R
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lida-R commented Feb 10, 2025

Hello there,

Is there any way to get both Gene count and transcript/isoform count file using --quantMode GeneCounts or --quantMode TranscriptomeSAM ?
I have already created the bam file and Gene count using --quantMode GeneCounts, but now I need to create the transcript file using RSEM. Should I re-start the mapping from the scratch or is there any way to create it using the current bam file?

Thank you for your help in advance,
Lida

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