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A template for bioinformatic and ecological analysis of metabarcoding

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bioinfo.starter

The goal of bioinfo.starter is to [...]

Installation

Clone from github

git clone [email protected]:adrientaudiere/bioinfo.starter.git
git checkout -b name_analyse

Adapt to your pipeline

  • Replace data/data_raw/metadata/sam_data.csv with good metadata file
  • Copy fastq files in data/data_raw/rawseq
  • Add references database in data/data_raw/refseq
  • Modify the _targets.R files (at least modify primers sequences and name of the reference database)
  • Modify (if necessary) params pattern_remove_sam_data and pattern_remove_fastq_files to make matching fastq files and sample names in metadata

Install R packages and run targets pipeline

renv::install()
targets::tar_make()

Colophon

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A template for bioinformatic and ecological analysis of metabarcoding

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