Skip to content

Commit

Permalink
Update Usage.md
Browse files Browse the repository at this point in the history
  • Loading branch information
huyustats authored Jan 28, 2021
1 parent 431c428 commit ef52ea7
Showing 1 changed file with 5 additions and 11 deletions.
16 changes: 5 additions & 11 deletions doc/Usage.md
Original file line number Diff line number Diff line change
Expand Up @@ -15,7 +15,7 @@ liqa -task <task>:
## Step 1: Transforming isoforms to compatible matrix based on reference annotation file
LIQA accepts two formats of reference annotation file. User can download the example reference file and data from the [example folder](https://github.com/WGLab/LIQA/tree/master/example):
### GTF format
For example:
For example `example.gtf`:
```
chr21 HAVANA gene 25639258 25717562 . + . gene_id "ENSG00000154721.15"; gene_type "protein_coding"; gene_name "JAM2"; level 2; hgnc_id "HGNC:14686"; tag "overlapping_locus"; havana_gene "OTTHUMG00000
078441.4";
Expand All @@ -30,17 +30,11 @@ ame "JAM2-206"; exon_number 1; exon_id "ENSE00003843052.1"; level 2; protein_id
911.1"; havana_gene "OTTHUMG00000078441.4"; havana_transcript "OTTHUMT00000171347.2";
```
### UCSC all fields
For example:
For example `example.ucsc`:
```
749 NM_001397 chr1 - 21543739 21616982 21546447 21616907 19 21543739,21548239,21551742,21553651,21554423,21560050,21562342,21563238,21564626,21571481,21573713,21582439,21584017,21585185
,21586763,21599191,21605683,21616562,21616856, 21546624,21548335,21551933,21553719,21554534,21560154,21562420,21563337,21564737,21571596,21573856,21582631,21584083,21585332,21586885,21599404,21605825,21616649,21616982, 0 ECE1cmpl cmpl 0,0,1,2,2,0,0,0,0,2,0,0,0,0,1,1,0,0,0,
93 NM_001113348 chr1 - 21543739 21672034 21546447 21671871 19 21543739,21548239,21551742,21553651,21554423,21560050,21562342,21563238,21564626,21571481,21573713,21582439,21584017,21585185
,21586763,21599191,21605683,21616562,21671868, 21546624,21548335,21551933,21553719,21554534,21560154,21562420,21563337,21564737,21571596,21573856,21582631,21584083,21585332,21586885,21599404,21605825,21616649,21672034, 0 ECE1cmpl cmpl 0,0,1,2,2,0,0,0,0,2,0,0,0,0,1,1,0,0,0,
749 NM_001113349 chr1 - 21543739 21616766 21546447 21616691 18 21543739,21548239,21551742,21553651,21554423,21560050,21562342,21563238,21564626,21571481,21573713,21582439,21584017,21585185
,21586763,21599191,21605683,21616562, 21546624,21548335,21551933,21553719,21554534,21560154,21562420,21563337,21564737,21571596,21573856,21582631,21584083,21585332,21586885,21599404,21605825,21616766, 0 ECE1 cmpl cmpl0,0,1,2,2,0,0,0,0,2,0,0,0,0,1,1,0,0,
749 NM_001113347 chr1 - 21543739 21606183 21546447 21605927 17 21543739,21548239,21551742,21553651,21554423,21560050,21562342,21563238,21564626,21571481,21573713,21582439,21584017,21585185
,21586763,21599191,21605683, 21546624,21548335,21551933,21553719,21554534,21560154,21562420,21563337,21564737,21571596,21573856,21582631,21584083,21585332,21586885,21599404,21606183, 0 ECE1 cmpl cmpl 0,0,1,2,2,0,0
,0,0,2,0,0,0,0,1,1,0,
947 ENST00000397748.5 chr21 - 47556175 47575481 47556367 47575437 15 47556175,47556828,47557152,47558421,47558793,47565330,47565731,47566179,47570032,47570301,47571471,47571805,47572820,47574062,47575383, 47556408,47556967,47557248,47558560,47558837,47565492,47565861,47566241,47570164,47570439,47571651,47571894,47572949,47574246,47575481, 0 FTCD cmpl cmpl 1,0,0,2,0,0,2,0,0,0,0,1,1,0,0,
947 ENST00000397743.1 chr21 - 47556692 47575481 47557159 47575437 13 47556692,47557152,47558421,47565330,47565731,47566179,47570032,47570301,47571471,47571805,47572820,47574062,47575383,47556987,47557248,47558560,47565492,47565861,47566241,47570164,47570439,47571651,47571894,47572949,47574246,47575481, 0 FTCD cmpl cmpl -1,1,0,0,2,0,0,0,0,1,1,0,0,
947 ENST00000397746.7 chr21 - 47556692 47575481 47556900 47575437 14 47556692,47557152,47558421,47558793,47565330,47565731,47566179,47570032,47570301,47571471,47571805,47572820,47574062,47575383, 47556987,47557248,47558560,47558837,47565492,47565861,47566241,47570164,47570439,47571651,47571894,47572949,47574246,47575481, 0 FTCD cmpl cmpl 0,0,2,0,0,2,0,0,0,0,1,1,0,0,
```
This reference file can be downloaded at [UCSC](https://genome.ucsc.edu/cgi-bin/hgTables?command=start) by selecting "all fields from selected table" in output format.

Expand Down

0 comments on commit ef52ea7

Please sign in to comment.