E coli K12 MG1655 Hybrid Assembly

Instrument:  PacBio RS
Chemistry:  C2
Enzyme: C2

Summary

This Escherichia coli K12 MG1655 dataset combines circular consensus sequencing (CCS) and continuous long reads (CLR). Download the data files to experiment with hybrid assembly. Data include:

• 1 SMRT Cell of 10 kbp long reads
• 16 SMRT Cells of 2 kbp CCS reads

With the data, you can try SMRT hybrid assembly two ways:

• Combine 1 SMRT Cell of 10 kbp long reads with 16 SMRT Cells of 2 kbp CCS reads
• Combine 1 SMRT Cell of 10 kbp long reads with Illumina data

To assemble the genome using long read correction and hybrid assembly, see the README below. You can also learn more from the technical notes and webinars on PacBio®'s de novo assembly application page.

Final (Secondary Analysis) Data

• E coli K12 De Novo README
• E. coli K12 De Novo FASTQ and Scripts 300 MB