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feat(hardware-testing): Abr Asair Script update (#16440)
# Overview <!-- Update to automated asair sensor script --> ## Test Plan and Hands on Testing <!-- Tested on ABR units to ensure automatic discovery and connection of USB0 --> ## Changelog <!-- Connects specifically to USB0 port, in order to run properly on robots using the plate reader --> ## Review requests <!-- - ABR_Asair drivers will still work as intended for other instances where it was used -->
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"""The package holding code for simulating protocols.""" |
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import re | ||
import sys | ||
import os | ||
from pathlib import Path | ||
from click import Context | ||
from opentrons.cli import analyze | ||
import json | ||
import argparse | ||
from datetime import datetime | ||
from abr_testing.automation import google_sheets_tool | ||
from abr_testing.data_collection import read_robot_logs | ||
from typing import Set, Dict, Any, Tuple, List, Union | ||
from abr_testing.tools import plate_reader | ||
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def look_for_air_gaps(protocol_file_path: str) -> int: | ||
instances = 0 | ||
try: | ||
with open(protocol_file_path, "r") as open_file: | ||
protocol_lines = open_file.readlines() | ||
for line in protocol_lines: | ||
if "air_gap" in line: | ||
print(line) | ||
instances += 1 | ||
print(f'Found {instances} instance(s) of the air gap function') | ||
open_file.close() | ||
except Exception as error: | ||
print("Error reading protocol:", error.with_traceback()) | ||
return instances | ||
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def set_api_level(protocol_file_path) -> None: | ||
with open(protocol_file_path, "r") as file: | ||
file_contents = file.readlines() | ||
# Look for current'apiLevel:' | ||
for i, line in enumerate(file_contents): | ||
print(line) | ||
if 'apiLevel' in line: | ||
print(f"The current API level of this protocol is: {line}") | ||
change = input("Would you like to simulate with a different API level? (Y/N) ").strip().upper() | ||
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if change == "Y": | ||
api_level = input("Protocol API Level to Simulate with: ") | ||
# Update new API level | ||
file_contents[i] = f'apiLevel: {api_level}\n' | ||
print(f"Updated line: {file_contents[i]}") | ||
break | ||
with open(protocol_file_path, "w") as file: | ||
file.writelines(file_contents) | ||
print("File updated successfully.") | ||
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original_exit = sys.exit | ||
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def mock_exit(code=None) -> None: | ||
print(f"sys.exit() called with code: {code}") | ||
raise SystemExit(code) | ||
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def get_labware_name(id: str, object_dict: dict, json_data: dict) -> str: | ||
slot = "" | ||
for obj in object_dict: | ||
if obj['id'] == id: | ||
try: | ||
# Try to get the slotName from the location | ||
slot = obj['location']['slotName'] | ||
return " SLOT: " + slot | ||
except KeyError: | ||
location = obj.get('location', {}) | ||
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# Check if location contains 'moduleId' | ||
if 'moduleId' in location: | ||
return get_labware_name(location['moduleId'], json_data['modules'], json_data) | ||
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# Check if location contains 'labwareId' | ||
elif 'labwareId' in location: | ||
return get_labware_name(location['labwareId'], json_data['labware'], json_data) | ||
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return " Labware not found" | ||
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def parse_results_volume(json_data_file: str) -> Tuple[ | ||
List[str], List[str], List[str], List[str], | ||
List[str], List[str], List[str], List[str], | ||
List[str], List[str], List[str] | ||
]: | ||
json_data = [] | ||
with open(json_data_file, "r") as json_file: | ||
json_data = json.load(json_file) | ||
commands = json_data.get("commands", []) | ||
start_time = datetime.fromisoformat(commands[0]["createdAt"]) | ||
end_time = datetime.fromisoformat(commands[len(commands)-1]["completedAt"]) | ||
header = ["", "Protocol Name", "Date", "Time"] | ||
header_fill_row = ["", protocol_name, str(file_date.date()), str(file_date.time())] | ||
labware_names_row =["Labware Name"] | ||
volume_dispensed_row =["Total Volume Dispensed uL"] | ||
volume_aspirated_row =["Total Volume Aspirated uL"] | ||
change_in_volume_row = ["Total Change in Volume uL"] | ||
start_time_row = ["Start Time"] | ||
end_time_row = ["End Time"] | ||
total_time_row = ["Total Time of Execution"] | ||
metrics_row = [ | ||
"Metric", | ||
"Heatershaker # of Latch Open/Close", | ||
"Heatershaker # of Homes", | ||
"Heatershaker # of Rotations", | ||
"Heatershaker Temp On Time (sec)", | ||
"Temp Module # of Temp Changes", | ||
"Temp Module Temp On Time (sec)", | ||
"Temp Mod Time to 4C (sec)", | ||
"Thermocycler # of Lid Open/Close", | ||
"Thermocycler Block # of Temp Changes", | ||
"Thermocycler Block Temp On Time (sec)", | ||
"Thermocycler Block Time to 4C (sec)", | ||
"Thermocycler Lid # of Temp Changes", | ||
"Thermocycler Lid Temp On Time (sec)", | ||
"Thermocycler Lid Time to 105C (sec)", | ||
"Plate Reader # of Reads", | ||
"Plate Reader Avg Read Time (sec)", | ||
"Plate Reader # of Initializations", | ||
"Plate Reader Avg Initialize Time (sec)", | ||
"Plate Reader # of Lid Movements", | ||
"Plate Reader Result", | ||
"Left Pipette Total Tip Pick Up(s)", | ||
"Left Pipette Total Aspirates", | ||
"Left Pipette Total Dispenses", | ||
"Right Pipette Total Tip Pick Up(s)", | ||
"Right Pipette Total Aspirates", | ||
"Right Pipette Total Dispenses", | ||
"Gripper Pick Ups", | ||
"Total Liquid Probes", | ||
"Average Liquid Probe Time (sec)", | ||
] | ||
values_row = ["Value"] | ||
labware_well_dict = {} | ||
hs_dict, temp_module_dict, thermo_cycler_dict, plate_reader_dict, instrument_dict = {}, {}, {}, {}, {} | ||
try: | ||
hs_dict = read_robot_logs.hs_commands(json_data) | ||
temp_module_dict = read_robot_logs.temperature_module_commands(json_data) | ||
thermo_cycler_dict = read_robot_logs.thermocycler_commands(json_data) | ||
plate_reader_dict = read_robot_logs.plate_reader_commands(json_data, hellma_plate_standards) | ||
instrument_dict = read_robot_logs.instrument_commands(json_data) | ||
except: | ||
pass | ||
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metrics = [hs_dict, temp_module_dict, thermo_cycler_dict, plate_reader_dict, instrument_dict] | ||
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# Iterate through all the commands executed in the protocol run log | ||
for x, command in enumerate(commands): | ||
if x != 0: | ||
prev_command = commands[x-1] | ||
if command["commandType"] == "aspirate": | ||
if not (prev_command["commandType"] == "comment" and (prev_command['params']['message'] == "AIR GAP" or prev_command['params']['message'] == "MIXING")): | ||
labware_id = command["params"]["labwareId"] | ||
labware_name = "" | ||
for labware in json_data.get("labware"): | ||
if labware["id"] == labware_id: | ||
labware_name = (labware["loadName"]) + get_labware_name(labware["id"], json_data["labware"], json_data) | ||
well_name = command["params"]["wellName"] | ||
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if labware_id not in labware_well_dict: | ||
labware_well_dict[labware_id] = {} | ||
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if well_name not in labware_well_dict[labware_id]: | ||
labware_well_dict[labware_id][well_name] = (labware_name, 0, 0, "") | ||
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vol = int(command["params"]["volume"]) | ||
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labware_name, added_volumes, subtracted_volumes, log = labware_well_dict[labware_id][well_name] | ||
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subtracted_volumes += vol | ||
log+=(f"aspirated {vol} ") | ||
labware_well_dict[labware_id][well_name] = (labware_name, added_volumes, subtracted_volumes, log) | ||
elif command["commandType"] == "dispense": | ||
if not (prev_command["commandType"] == "comment" and (prev_command['params']['message'] == "MIXING")): | ||
labware_id = command["params"]["labwareId"] | ||
labware_name = "" | ||
for labware in json_data.get("labware"): | ||
if labware["id"] == labware_id: | ||
labware_name = (labware["loadName"]) + get_labware_name(labware["id"], json_data["labware"], json_data) | ||
well_name = command["params"]["wellName"] | ||
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if labware_id not in labware_well_dict: | ||
labware_well_dict[labware_id] = {} | ||
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if well_name not in labware_well_dict[labware_id]: | ||
labware_well_dict[labware_id][well_name] = (labware_name, 0, 0, "") | ||
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vol = int(command["params"]["volume"]) | ||
labware_name, added_volumes, subtracted_volumes, log = labware_well_dict[labware_id][well_name] | ||
added_volumes += vol | ||
log+=(f"dispensed {vol} ") | ||
labware_well_dict[labware_id][well_name] = (labware_name, added_volumes, subtracted_volumes, log) | ||
# file_date_formatted = file_date.strftime("%Y-%m-%d_%H-%M-%S") | ||
with open(f"{os.path.dirname(json_data_file)}\\{protocol_name}_well_volumes_{file_date_formatted}.json", "w") as output_file: | ||
json.dump(labware_well_dict, output_file) | ||
output_file.close() | ||
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# populate row lists | ||
for labware_id in labware_well_dict.keys(): | ||
volume_added = 0 | ||
volume_subtracted = 0 | ||
labware_name ="" | ||
for well in labware_well_dict[labware_id].keys(): | ||
labware_name, added_volumes, subtracted_volumes, log = labware_well_dict[labware_id][well] | ||
volume_added += added_volumes | ||
volume_subtracted += subtracted_volumes | ||
labware_names_row.append(labware_name) | ||
volume_dispensed_row.append(str(volume_added)) | ||
volume_aspirated_row.append(str(volume_subtracted)) | ||
change_in_volume_row.append(str(volume_added - volume_subtracted)) | ||
start_time_row.append(str(start_time.time())) | ||
end_time_row.append(str(end_time.time())) | ||
total_time_row.append(str(end_time - start_time)) | ||
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for metric in metrics: | ||
for cmd in metric.keys(): | ||
values_row.append(str(metric[cmd])) | ||
return( | ||
header, | ||
header_fill_row, | ||
labware_names_row, | ||
volume_dispensed_row, | ||
volume_aspirated_row, | ||
change_in_volume_row, | ||
start_time_row, | ||
end_time_row, | ||
total_time_row, | ||
metrics_row, | ||
values_row) | ||
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def main(storage_directory, google_sheet_name, protocol_file_path): | ||
sys.exit = mock_exit | ||
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# Read file path from arguments | ||
protocol_file_path = Path(protocol_file_path) | ||
global protocol_name | ||
protocol_name = protocol_file_path.stem | ||
print("Simulating", protocol_name) | ||
global file_date | ||
file_date = datetime.now() | ||
global file_date_formatted | ||
file_date_formatted = file_date.strftime("%Y-%m-%d_%H-%M-%S") | ||
# Prepare output file | ||
json_file_path = f"{storage_directory}\\{protocol_name}_{file_date_formatted}.json" | ||
json_file_output = open(json_file_path, "wb+") | ||
error_output = f"{storage_directory}\\error_log" | ||
# Run protocol simulation | ||
try: | ||
with Context(analyze) as ctx: | ||
ctx.invoke( | ||
analyze, | ||
files=[protocol_file_path], | ||
json_output=json_file_output, | ||
human_json_output=None, | ||
log_output=error_output, | ||
log_level="ERROR", | ||
check=False | ||
) | ||
except SystemExit as e: | ||
print(f"SystemExit caught with code: {e}") | ||
finally: | ||
sys.exit = original_exit | ||
json_file_output.close() | ||
with open(error_output, "r") as open_file: | ||
try: | ||
errors = open_file.readlines() | ||
if not errors: pass | ||
else: | ||
print(errors) | ||
sys.exit(1) | ||
except: | ||
print("error simulating ...") | ||
sys.exit() | ||
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try: | ||
credentials_path = os.path.join(storage_directory, "credentials.json") | ||
print(credentials_path) | ||
except FileNotFoundError: | ||
print(f"Add credentials.json file to: {storage_directory}.") | ||
sys.exit() | ||
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global hellma_plate_standards | ||
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try: | ||
hellma_plate_standards = plate_reader.read_hellma_plate_files(storage_directory, 101934) | ||
except: | ||
print(f"Add helma plate standard files to {storage_directory}.") | ||
sys.exit() | ||
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google_sheet = google_sheets_tool.google_sheet( | ||
credentials_path, google_sheet_name, 0 | ||
) | ||
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google_sheet.write_to_row([]) | ||
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for row in parse_results_volume(json_file_path): | ||
print("Writing results to", google_sheet_name) | ||
print(str(row)) | ||
google_sheet.write_to_row(row) | ||
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if __name__ == "__main__": | ||
CLEAN_PROTOCOL = True | ||
parser = argparse.ArgumentParser(description="Read run logs on google drive.") | ||
parser.add_argument( | ||
"storage_directory", | ||
metavar="STORAGE_DIRECTORY", | ||
type=str, | ||
nargs=1, | ||
help="Path to long term storage directory for run logs.", | ||
) | ||
parser.add_argument( | ||
"sheet_name", | ||
metavar="SHEETNAME", | ||
type=str, | ||
nargs=1, | ||
help="Name of sheet to upload results to", | ||
) | ||
parser.add_argument( | ||
"protocol_file_path", | ||
metavar="PROTOCOL_FILE_PATH", | ||
type=str, | ||
nargs=1, | ||
help="Path to protocol file" | ||
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) | ||
args = parser.parse_args() | ||
storage_directory = args.storage_directory[0] | ||
sheet_name = args.sheet_name[0] | ||
protocol_file_path = args.protocol_file_path[0] | ||
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SETUP = True | ||
while(SETUP): | ||
print("This current version cannot properly handle air gap calls.\nThese may cause simulation results to be inaccurate") | ||
air_gaps = look_for_air_gaps(protocol_file_path) | ||
if air_gaps > 0: | ||
choice = "" | ||
while not choice: | ||
choice = input("This protocol contains air gaps, results may be innacurate, would you like to continue? (Y/N): ") | ||
if choice.upper() == "Y": | ||
SETUP = False | ||
CLEAN_PROTOCOL = True | ||
elif choice.upper() == "N": | ||
CLEAN_PROTOCOL = False | ||
SETUP = False | ||
print("Please remove air gaps then re-run") | ||
else: | ||
choice = "" | ||
print("Please enter a valid response.") | ||
SETUP = False | ||
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if CLEAN_PROTOCOL: | ||
main( | ||
storage_directory, | ||
sheet_name, | ||
protocol_file_path, | ||
) | ||
else: sys.exit(0) |
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