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MultiGPU notebooks calculated differentially expressed PCAs #99

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Intron7 opened this issue Sep 8, 2022 · 1 comment
Closed

MultiGPU notebooks calculated differentially expressed PCAs #99

Intron7 opened this issue Sep 8, 2022 · 1 comment

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@Intron7
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Intron7 commented Sep 8, 2022

Hey Nvidia Genomics Team,

In the Multi GPU Notebook the AnnData object post PCA is created with the PCA in .X. Therefore when you later later perform the differential gene expression you calculate the which PCA is the most import for each cluster.

The ranked gene groups function still has a couple of bugs. There is a PR with a fix. Which also introduces some performance improvements.

Yours Severin

@111kakaluote
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I create anndata with lognormalized and scaledata, like this:

sparse_gpu_array_scale = dask_sparse_arr.compute()  #sparse_gpu_array_scale store scaledata
adata = anndata.AnnData(sparse_gpu_array_scale.get())
adata.layers["normalised"] = sparse_gpu_array.get()  #sparse_gpu_array store lognormalized data
adata.var_names = genes.to_pandas()
adata.obsm['X_pca'] = local_pca.get()

so I can ranked gene groups with adata.X. however, which the newest verison of rapids-single-cell-example? github or docker images?

Intron7 added a commit to Intron7/rapids-single-cell-examples that referenced this issue Dec 5, 2022
@Intron7 Intron7 closed this as completed Dec 19, 2022
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