Understanding the role of fibroblasts in the tumor microenvironment (TME) of human liver cancer is crucial for developing therapeutic methods specifically targeting tumor-promoting CAFs. Here, we describe a CAF subpopulation associated with cancer stemness and immunosuppressive TME by directly interacting with liver cancer stem cells or inhibitory M2 macrophages.
This code was developed in R version (4.1.3) for statistical analyses of spatial transcriptomics data and proteomics data. The detail infomation can refer to Supplementary Methods.
Quantitative data for the proteome can be obtained from /proteome/DATA_all_sample.xls
Raw spatial transcriptomics data can be obtained from the paper and/or the Supplementary Materials.
If you use code or data in this project, please cite:
- Siyu, J. 2023. "Spatial multiomics analysis reveals a subpopulation of fibroblasts associated with immunosuppression and cancer stemness in human liver cancer".
We also thank Xufeng Chen, Ping Lin for their contribution.