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fix links to Mcap_PaxC plots
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kubu4 committed Jan 5, 2024
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4 changes: 2 additions & 2 deletions docs/index.html
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Expand Up @@ -183,9 +183,9 @@ <h5 class="quarto-listing-category-title">Categories</h5><div class="quarto-list

<div class="quarto-listing quarto-listing-container-default" id="listing-listing">
<div class="list quarto-listing-default">
<div class="quarto-post image-right" data-index="0" data-categories="qPCR,CFX Connect,2023" data-listing-date-sort="1700380800000" data-listing-file-modified-sort="1704493185361" data-listing-date-modified-sort="NaN" data-listing-reading-time-sort="12">
<div class="quarto-post image-right" data-index="0" data-categories="qPCR,CFX Connect,2023" data-listing-date-sort="1700380800000" data-listing-file-modified-sort="1704493482908" data-listing-date-modified-sort="NaN" data-listing-reading-time-sort="12">
<div class="thumbnail">
<p><a href="./posts/2023/2023-11-19-qPCR---Initial-Primer-Tests-with-Positive-Control-Serial-Dilutions/index.html"> <p class="card-img-top"><img src="data/qPCR/testing/exported/sam_2023-11-19-08-35-30-CAct-Amplification_plot.png" alt="CAct Amplificaiton plot showing a single sample amplification of 100pg input DNA at Cq ~= 39" class="thumbnail-image card-img"/></p> </a></p>
<p><a href="./posts/2023/2023-11-19-qPCR---Initial-Primer-Tests-with-Positive-Control-Serial-Dilutions/index.html"> <p class="card-img-top"><img src="data/qPCR/testing/exported/sam_2023-11-19-08-35-30-CAct-Amplification_plot.png" alt="CAct Amplification plot showing a single sample amplification of 100pg input DNA at Cq ~= 39" class="thumbnail-image card-img"/></p> </a></p>
</div>
<div class="body">
<a href="./posts/2023/2023-11-19-qPCR---Initial-Primer-Tests-with-Positive-Control-Serial-Dilutions/index.html">
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6 changes: 3 additions & 3 deletions docs/index.xml
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Expand Up @@ -156,7 +156,7 @@
</thead>
<tbody>
<tr class="odd">
<td><img src="https://jpglabhome.github.io/SymbID_protbleach/data/qPCR/testing/exported/sam_2023-11-19-08-35-30-CAct-Amplification_plot.png" class="img-fluid" alt="CAct Amplificaiton plot showing a single sample amplification of 100pg input DNA at Cq ~= 39"></td>
<td><img src="https://jpglabhome.github.io/SymbID_protbleach/data/qPCR/testing/exported/sam_2023-11-19-08-35-30-CAct-Amplification_plot.png" class="img-fluid" alt="CAct Amplification plot showing a single sample amplification of 100pg input DNA at Cq ~= 39"></td>
<td><img src="https://jpglabhome.github.io/SymbID_protbleach/data/qPCR/testing/exported/sam_2023-11-19-08-35-30-CAct-Melt_plot.png" class="img-fluid" alt="CAct melt plot showing a single melt curve"></td>
</tr>
</tbody>
Expand All @@ -178,7 +178,7 @@
</thead>
<tbody>
<tr class="odd">
<td><img src="https://jpglabhome.github.io/SymbID_protbleach/data/qPCR/testing/exported/sam_2023-11-19-08-35-30-DAct-Amplification_plot.png" class="img-fluid" alt="DAct Amplificaiton plot showing sample amplification of 1000pg (yellow lines) and 100pg (pink lines) of input DNA"></td>
<td><img src="https://jpglabhome.github.io/SymbID_protbleach/data/qPCR/testing/exported/sam_2023-11-19-08-35-30-DAct-Amplification_plot.png" class="img-fluid" alt="DAct Amplification plot showing sample amplification of 1000pg (yellow lines) and 100pg (pink lines) of input DNA"></td>
<td><img src="https://jpglabhome.github.io/SymbID_protbleach/data/qPCR/testing/exported/sam_2023-11-19-08-35-30-DAct-Melt_plot.png" class="img-fluid" alt="DAct melt plot showing melt curves of 1000pg (yellow lines) and 100pg (pink lines) of input DNA"></td>
</tr>
</tbody>
Expand All @@ -200,7 +200,7 @@
</thead>
<tbody>
<tr class="odd">
<td><img src="https://jpglabhome.github.io/SymbID_protbleach/data/qPCR/testing/data/qPCR/testing/data/qPCR/testing/exported/sam_2023-11-19-08-35-30-CAct-Melt_plot.png)" class="img-fluid" alt="Mcap_paxC Amplificaiton plot showing sample amplification of 1000pg (yellow lines) and 100pg (pink lines) of input DNA"></td>
<td><img src="https://jpglabhome.github.io/SymbID_protbleach/data/qPCR/testing/data/qPCR/testing/data/qPCR/testing/exported/sam_2023-11-19-08-35-30-CAct-Melt_plot.png)" class="img-fluid" alt="Mcap_paxC Amplification plot showing sample amplification of 1000pg (yellow lines) and 100pg (pink lines) of input DNA"></td>
<td><img src="https://jpglabhome.github.io/SymbID_protbleach/data/qPCR/testing/exported/sam_2023-11-19-08-35-30-Mcap_PaxC-Melt_plot.png" class="img-fluid" alt="Mcap_paxC melt plot showing melt curves of 1000pg (yellow lines) and 100pg (pink lines) of input DNA"></td>
</tr>
</tbody>
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Expand Up @@ -351,7 +351,7 @@ <h2 class="anchored" data-anchor-id="cact-amplification">CAct Amplification</h2>
</thead>
<tbody>
<tr class="odd">
<td><img src="../../../data/qPCR/testing/exported/sam_2023-11-19-08-35-30-CAct-Amplification_plot.png" class="img-fluid" alt="CAct Amplificaiton plot showing a single sample amplification of 100pg input DNA at Cq ~= 39"></td>
<td><img src="../../../data/qPCR/testing/exported/sam_2023-11-19-08-35-30-CAct-Amplification_plot.png" class="img-fluid" alt="CAct Amplification plot showing a single sample amplification of 100pg input DNA at Cq ~= 39"></td>
<td><img src="../../../data/qPCR/testing/exported/sam_2023-11-19-08-35-30-CAct-Melt_plot.png" class="img-fluid" alt="CAct melt plot showing a single melt curve"></td>
</tr>
</tbody>
Expand All @@ -373,7 +373,7 @@ <h2 class="anchored" data-anchor-id="dact-amplification">DAct Amplification</h2>
</thead>
<tbody>
<tr class="odd">
<td><img src="../../../data/qPCR/testing/exported/sam_2023-11-19-08-35-30-DAct-Amplification_plot.png" class="img-fluid" alt="DAct Amplificaiton plot showing sample amplification of 1000pg (yellow lines) and 100pg (pink lines) of input DNA"></td>
<td><img src="../../../data/qPCR/testing/exported/sam_2023-11-19-08-35-30-DAct-Amplification_plot.png" class="img-fluid" alt="DAct Amplification plot showing sample amplification of 1000pg (yellow lines) and 100pg (pink lines) of input DNA"></td>
<td><img src="../../../data/qPCR/testing/exported/sam_2023-11-19-08-35-30-DAct-Melt_plot.png" class="img-fluid" alt="DAct melt plot showing melt curves of 1000pg (yellow lines) and 100pg (pink lines) of input DNA"></td>
</tr>
</tbody>
Expand All @@ -395,7 +395,7 @@ <h2 class="anchored" data-anchor-id="mcap_paxc-amplification">Mcap_PaxC Amplific
</thead>
<tbody>
<tr class="odd">
<td><img src="../../../data/qPCR/testing/exported/Amplification Plot Melt Plot ![DAct Amplificaiton plot showing sample amplification of 1000pg (yellow lines) and 100pg (pink lines) of input DNA at Cq ~= 39](../../../data/qPCR/testing/exported/sam_2023-11-19-08-35-30-DAct-Amplification_plot.png) ![CAct melt plot showing melt curves of 1000pg (yellow lines) and 100pg (pink lines) of input DNA](../../../data/qPCR/testing/exported/sam_2023-11-19-08-35-30-CAct-Melt_plot.png)" class="img-fluid" alt="Mcap_paxC Amplificaiton plot showing sample amplification of 1000pg (yellow lines) and 100pg (pink lines) of input DNA"></td>
<td><img src="../../../data/qPCR/testing/exported/Amplification Plot Melt Plot ![Mcap_PaxC Amplification plot showing sample amplification of 1000pg (yellow lines) and 100pg (pink lines) of input DNA](../../../data/qPCR/testing/exported/sam_2023-11-19-08-35-30-Mcap_PaxC-Amplification_plot.png) ![Mcap_PaxC melt plot showing melt curves of 1000pg (yellow lines) and 100pg (pink lines) of input DNA](../../../data/qPCR/testing/exported/sam_2023-11-19-08-35-30-CAct-Melt_plot.png)" class="img-fluid" alt="Mcap_paxC Amplification plot showing sample amplification of 1000pg (yellow lines) and 100pg (pink lines) of input DNA"></td>
<td><img src="../../../data/qPCR/testing/exported/sam_2023-11-19-08-35-30-Mcap_PaxC-Melt_plot.png" class="img-fluid" alt="Mcap_paxC melt plot showing melt curves of 1000pg (yellow lines) and 100pg (pink lines) of input DNA"></td>
</tr>
</tbody>
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4 changes: 2 additions & 2 deletions docs/sitemap.xml
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Expand Up @@ -10,10 +10,10 @@
</url>
<url>
<loc>https://jpglabhome.github.io/SymbID_protbleach/posts/2023/2023-11-19-qPCR---Initial-Primer-Tests-with-Positive-Control-Serial-Dilutions/index.html</loc>
<lastmod>2024-01-05T22:20:10.525Z</lastmod>
<lastmod>2024-01-05T22:24:54.023Z</lastmod>
</url>
<url>
<loc>https://jpglabhome.github.io/SymbID_protbleach/index.html</loc>
<lastmod>2024-01-05T22:20:11.401Z</lastmod>
<lastmod>2024-01-05T22:24:54.855Z</lastmod>
</url>
</urlset>
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Expand Up @@ -77,23 +77,23 @@ All qPCRs were run in triplicate for each dilution (1000pg, 100pg, 10pg), with S

| Amplification Plot | Melt Plot |
|-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------|-------------------------------------------------------------------------------------------------------------------------------|
| ![CAct Amplificaiton plot showing a single sample amplification of 100pg input DNA at Cq ~= 39](../../../data/qPCR/testing/exported/sam_2023-11-19-08-35-30-CAct-Amplification_plot.png) | ![CAct melt plot showing a single melt curve](../../../data/qPCR/testing/exported/sam_2023-11-19-08-35-30-CAct-Melt_plot.png) |
| ![CAct Amplification plot showing a single sample amplification of 100pg input DNA at Cq ~= 39](../../../data/qPCR/testing/exported/sam_2023-11-19-08-35-30-CAct-Amplification_plot.png) | ![CAct melt plot showing a single melt curve](../../../data/qPCR/testing/exported/sam_2023-11-19-08-35-30-CAct-Melt_plot.png) |

Here we see a single sample amplifying, which corresponds to 100pg of input DNA. It's odd there's no amplification in any of the 1000pg samples, considering we got late amplification in one of the 100pg samples... Will have to re-run. Possibly will order fresh primer stocks.

## DAct Amplification

| Amplification Plot | Melt Plot |
|---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------|------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------|
| ![DAct Amplificaiton plot showing sample amplification of 1000pg (yellow lines) and 100pg (pink lines) of input DNA](../../../data/qPCR/testing/exported/sam_2023-11-19-08-35-30-DAct-Amplification_plot.png) | ![DAct melt plot showing melt curves of 1000pg (yellow lines) and 100pg (pink lines) of input DNA](../../../data/qPCR/testing/exported/sam_2023-11-19-08-35-30-DAct-Melt_plot.png) |
| ![DAct Amplification plot showing sample amplification of 1000pg (yellow lines) and 100pg (pink lines) of input DNA](../../../data/qPCR/testing/exported/sam_2023-11-19-08-35-30-DAct-Amplification_plot.png) | ![DAct melt plot showing melt curves of 1000pg (yellow lines) and 100pg (pink lines) of input DNA](../../../data/qPCR/testing/exported/sam_2023-11-19-08-35-30-DAct-Melt_plot.png) |

We see all three reps of 1000pg DNA amplifying (yellow lines) and two of the three 100pg reps amplifying (pink lines). No amplification with 10pg of input DNA. Melt curves look good.

## Mcap_PaxC Amplification

| Amplification Plot | Melt Plot |
|-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------|----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------|
| ![Mcap_paxC Amplificaiton plot showing sample amplification of 1000pg (yellow lines) and 100pg (pink lines) of input DNA](../../../data/qPCR/testing/exported/Amplification Plot Melt Plot ![DAct Amplificaiton plot showing sample amplification of 1000pg (yellow lines) and 100pg (pink lines) of input DNA at Cq ~= 39](../../../data/qPCR/testing/exported/sam_2023-11-19-08-35-30-DAct-Amplification_plot.png) ![CAct melt plot showing melt curves of 1000pg (yellow lines) and 100pg (pink lines) of input DNA](../../../data/qPCR/testing/exported/sam_2023-11-19-08-35-30-CAct-Melt_plot.png) ) | ![Mcap_paxC melt plot showing melt curves of 1000pg (yellow lines) and 100pg (pink lines) of input DNA](../../../data/qPCR/testing/exported/sam_2023-11-19-08-35-30-Mcap_PaxC-Melt_plot.png) |
| ![Mcap_paxC Amplification plot showing sample amplification of 1000pg (yellow lines) and 100pg (pink lines) of input DNA](../../../data/qPCR/testing/exported/Amplification Plot Melt Plot ![Mcap_PaxC Amplification plot showing sample amplification of 1000pg (yellow lines) and 100pg (pink lines) of input DNA](../../../data/qPCR/testing/exported/sam_2023-11-19-08-35-30-Mcap_PaxC-Amplification_plot.png) ![Mcap_PaxC melt plot showing melt curves of 1000pg (yellow lines) and 100pg (pink lines) of input DNA](../../../data/qPCR/testing/exported/sam_2023-11-19-08-35-30-CAct-Melt_plot.png) ) | ![Mcap_paxC melt plot showing melt curves of 1000pg (yellow lines) and 100pg (pink lines) of input DNA](../../../data/qPCR/testing/exported/sam_2023-11-19-08-35-30-Mcap_PaxC-Melt_plot.png) |

We see all three reps of 1000pg DNA amplifying (yellow lines) and all three 100pg reps amplifying (pink lines). No amplification with 10pg of input DNA. Melt curves look good.

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