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A plugin for Micro-Manager for automated acquisition of Partial Wave Spectroscopic Microscopy data.

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mmPWSPlugin

This repository contains the Micro-Manager plugins that are used for PWS acquisition.

PWS Plugin

Acquisition Sequencer

A tree based acquisition engine allows extremely flexible automated imaging.

Reliable hardware autofocus.

The reliability of Hardware Autofocus ZStage devices during automated imaging are enhanced with Software Autofocus in the event of a hardware fault.

Utilities

Utilities provide automatic exposure time reccomendation, autofocus. More to come.

Hardware Abstraction

Enhanced handling of specral cameras, Nikon PFS ZStages, Illumination sources.

Image Sharpness Plugin

This plugin appears as a panel in the "Image Inspector". It provides a graph of image sharpness vs Z position. This helps with the vital task of focusing images before PWS acquisition.

Dependencies

The plugins have a few dependencies that are not included with Micro-Manager: 1: Twelve-Monkeys TIFF plugin for ImageIO. These can be downloaded from https://github.com/haraldk/TwelveMonkeys. The required files are:

  • common-image
  • common-io
  • common-lang
  • imageio-core
  • imageio-metadata
  • imageio-tiff

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A plugin for Micro-Manager for automated acquisition of Partial Wave Spectroscopic Microscopy data.

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