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Merge pull request #104 from ARTbio/limma_data_manipulation
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start comparisons and fix typos
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drosofff authored Feb 15, 2024
2 parents 22bd137 + 1b030f2 commit 42de600
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6 changes: 3 additions & 3 deletions docs/bulk_RNAseq-IOC/28_1_manipulation_edgeR_data.md
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# Manipulation of edgeR data for visualisation and comparisons

Now we would like to extract the most differentially expressed genes in the various
As we did for DESeq2 we will extract the most differentially expressed genes in the various
conditions, and then visualize them using an heatmap of the normalized counts for each
sample.

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:warning: Rename the collection `Select on collection...` to `edgeR top down-regulated
gene lists`

:warning: keep the last three generated collections for later comparison with edgeR and
:warning: keep the last three generated collections for later comparison with DESeq2 and
limma tools

## Plotting an heatmap of the most significantly de-regulated genes
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!!! info "![](images/tool_small.png){width="25" align="absbottom"} `Plot heatmap with high number of rows` settings"
- Input should have column headers - these will be the columns that are plotted

--> `rLog-Normalized counts of DE genes`
--> Click the collection icon and select `edgeR Log2CPM Normalized counts of DE genes`
- Data transformation

--> `Plot the data as it is`
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