Possible to calculate burst kinetics for biallelic non-bursty and monoallelic genes

[Hemantcnaik]

Hello using scale how can we calculate burst kinetics for biallic non-bursty and monoallic genes I have run scale edited gene category R script changed it to all category name to "biallelic.bursty" so that get the burst frequency and burst size for a gene. my main concern is to get Burst frequency and size when I am doing this when I compare the true biallelic bursty genes the values are changing please can u help me why the burst kinetics are changing bellow pasting some output

https://drive.google.com/file/d/1azraUQstAf0S_zGMB42Juun0KU-kSPIZ/view?usp=sharing
Thank you