diff --git a/Pipfile.lock b/Pipfile.lock
index 7b79d98..e694713 100644
--- a/Pipfile.lock
+++ b/Pipfile.lock
@@ -111,7 +111,7 @@
                 "sha256:5a3d016c7c547f69d6f81fb0db9449ce888b418b5b9952cc5e6e66843e9dd845",
                 "sha256:83e9a75d1911279afd89352c68b45348559d1fc0506b054b346651b5e7fee29f"
             ],
-            "markers": "python_version >= '3.6'",
+            "markers": "python_full_version >= '3.6.0'",
             "version": "==2.1.1"
         },
         "click": {
@@ -132,78 +132,78 @@
         },
         "contourpy": {
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+                "sha256:f6ca38dd8d988eca8f07305125dec6f54ac1c518f1aaddcc14d08c01aebb6efc"
             ],
             "markers": "python_version >= '3.7'",
-            "version": "==1.0.5"
+            "version": "==1.0.6"
         },
         "cooler": {
             "hashes": [
@@ -228,27 +228,27 @@
         },
         "dill": {
             "hashes": [
-                "sha256:33501d03270bbe410c72639b350e941882a8b0fd55357580fbc873fba0c59302",
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-            "markers": "python_version >= '2.7' and python_version not in '3.0, 3.1, 3.2, 3.3, 3.4, 3.5, 3.6'",
-            "version": "==0.3.5.1"
+            "markers": "python_version >= '3.7'",
+            "version": "==0.3.6"
         },
         "filetype": {
             "hashes": [
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             ],
             "index": "pypi",
-            "version": "==1.1.0"
+            "version": "==1.2.0"
         },
         "fonttools": {
             "hashes": [
-                "sha256:86918c150c6412798e15a0de6c3e0d061ddefddd00f97b4f7b43dfa867ad315e",
-                "sha256:afae1b39555f9c3f0ad1f0f1daf678e5ad157e38c8842ecb567951bf1a9b9fd7"
+                "sha256:2bb244009f9bf3fa100fc3ead6aeb99febe5985fa20afbfbaa2f8946c2fbdaf1",
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             ],
             "markers": "python_version >= '3.7'",
-            "version": "==4.37.4"
+            "version": "==4.38.0"
         },
         "h5py": {
             "hashes": [
@@ -382,107 +382,106 @@
         },
         "matplotlib": {
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@@ -561,69 +560,70 @@
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@@ -662,30 +662,30 @@
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@@ -697,10 +697,10 @@
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@@ -793,78 +793,78 @@
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             "hashes": [
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-                "sha256:b10556817f09d46d420edd982dd0653940b90151d0576f09143a8e773459f6fe",
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-                "sha256:cf6e7d5fe2aeb54898df18db1baf479863eae581cce05410f61f6b4188c8ada1",
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-                "sha256:d74ee72b5071818a1a5dab47338e87f08a738cb938a3b0653b9e4d959ddd1fd9",
-                "sha256:dd16302d39c4d6f4afde80edd0c97d4db643327d355a312762ccd9bd2ca515ed",
-                "sha256:dd2fb11922f58df8528adfca123f6a84748ad17d066007e7ac977720063556bd",
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-                "sha256:fb62d517a516128bacf08cb6a86ecd39fb06d08e7c4980251f5d5601d29989ba"
+                "sha256:002f069c7bb9a86826616a78f1214fea5b993435720990eecb0bf10955b9cd0e",
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+                "sha256:0de746c8f76355c79fd15eccd7ecde0b137cd911bdcdc463fc5c36ec3d8b98ea",
+                "sha256:0f33d16fa7b5e2ed6ea85d7b31bc84cf8c73c40cc2c9f87071e0fffcd52f5342",
+                "sha256:0f49858b5fc802081b71269f4a3aa5c5500ec6553637c9a0630f30a2a6541ea7",
+                "sha256:17dbc7f71fa5b7e4a2acef38cf0be30461ae6659456a978ce7eeebeb5bdf9e1a",
+                "sha256:17ec5e408fb6615250c1f18fb4eac3b2b99a85e8613bfc2dfa54827d0bf7f3e1",
+                "sha256:1b4085151e00ab7ca66f269aff7153f0ec18589cb22e7ceb8b365709c723fdd0",
+                "sha256:1f169402069f8cf93e359f607725b1d920c4dbe5bda4c520025d5fad8d20c1b7",
+                "sha256:1fbacdbba3cf5a471c67a9ca6cd270bba9578d5bc22aef6028faebbdb98bbb15",
+                "sha256:252f7cc5524bb5507a08377a4a75aa7ff4645f3dfca814d38bdbcf0f3c34d1ce",
+                "sha256:2aeed35db00cdf5d49ff1e7d878afd38c86a5fead0f1d364d539ad4d7a869e0e",
+                "sha256:2cc76435569e6c19574a8e913cfccbed832249b2b3b360caee9a4caf8ff866bf",
+                "sha256:448ab14fa67b3ac235a8445d14ec6d56268c3dabbce78720f9efa6d698466710",
+                "sha256:4609feb2ae66c132c6dcbe01dbfd4f6431afb4ff17303e37ca128fb6297cebd2",
+                "sha256:46bafa7e794f0e91fde850d906b0dc29a624c726b27e75d23bc8c3e35a48f28b",
+                "sha256:4a6199d302ec7d889e1aa6b493aa8e40b4dfa4bd85708f8c8f0c64ce5b8e0986",
+                "sha256:4d8d016f70d241f82189bc9f6d1eb8558b3599861f2c501b3f32da7fdf4e92ac",
+                "sha256:503da91993cc671fe7ebbf120c3ce868278de8226f158336afde874f7b7aa871",
+                "sha256:54c63cc7857f16a20aa170ffda9ebce45a3b7ba764b67a5a95bfe7ae613a2710",
+                "sha256:58a429d2c2fa80834115b923ff689622de8f214cf0dc4afa9f59e824b444ab31",
+                "sha256:599e9c53d3203bc36ef68efec138ca76d201da7ac06a114fae78536a8c10e35b",
+                "sha256:5f3dd31309ae5cc9f2df51d2d5cac89722dac3c853042ebefcaf7ad06ca19387",
+                "sha256:6187cbea7fdede732fe0347ad08cd920ebd9faa30b6c48782cee494051ca97c6",
+                "sha256:622cf0e1f870f189a0757fdcad7998a0c1dd46b0e53aeac9960556c141319c83",
+                "sha256:638bdd2deaccd3b8e02b1783280bd82341df5e1faa59c4f0276f03f16eec13ea",
+                "sha256:6804ad50aaf581df5c982fc101b0d932638066fe191074ded783602eb1c8982a",
+                "sha256:7a4d9b266ae6db578719f1255c742e76ee4676593087f4f6b79a2bbae2b1dcc5",
+                "sha256:7a9476dcd72aeba7d55c4800b9cd2204201af3539894b8512d74597e35a3033a",
+                "sha256:7b95c5cf71c16e4fdaa724719aaf8ccbed533e2df57a20bcff825ceeead27688",
+                "sha256:8493d2c1a940471b07d7c9c356a3f4eee780df073da2917418d0fe8669b54f99",
+                "sha256:875cfb43b622672218045dc927a86fc7c4c8111264c1d303aca5de33d5df479e",
+                "sha256:8d762267c4af617e1798bd0151f626105d06a88f214e3874b77eb89106f899fe",
+                "sha256:94c17d01e4c65e63deec46c984bb810de5e3a1259eb6bacdca63f3efc9c4c673",
+                "sha256:96979ff7f0daf47422d5f95d2d006da3210e0490a166bce2529f59f55047fc67",
+                "sha256:97139bf5134d713710665a6edb9500d69b93642c4b6b44b20800232dbd0f5b39",
+                "sha256:989b31d586954e65170ad3ec597218a6790c401b82da6193e8a897a06aa7946e",
+                "sha256:98b4c824f15436f1b22fe6d73c42ffacb246f7efc4d9dbbee542dd72355ecc43",
+                "sha256:9aff3c24017a7819c76b2f177d4fe8334b3d4cb6f702a2d7c666b3d57c36ffb4",
+                "sha256:9db78e18624f94d7b5642bf487244f803dab844e771d92e83f85f22da21ffe2d",
+                "sha256:a0e6dd5a0b8c76fb7522470789f1af793d39d6edbd4e40853e7be550ad49c430",
+                "sha256:a2f70d8170c7e02166a4c91462581e6ae5f35e3351a6b6c5142adcb04c7153ac",
+                "sha256:a814227fa08cae435ac7a42dcd2a04a7ec4a3cee23b7f83f9544cd26f452dcc4",
+                "sha256:aa9ecdd1d7ecbc7d1066c37cfbe52f65adf64b11b22d481a98fe1d3675dfff4b",
+                "sha256:b2b19d7aa4e9a1e7bf8caaf5f478a790190c60136314f45bb7702cb5a9337266",
+                "sha256:b4997bd8332cef3923402a07351571788f552f55ea1394ffbfccd4d203a8a05f",
+                "sha256:b71fef8ee41d59509c7f4afac7f627ed143c9e6db9eb08cfbba85e4c4dc5e67b",
+                "sha256:bd67d6fad7f4cd7c9cb7fad32d78ce32862fdb574b898447987a5de22fd37d73",
+                "sha256:ca22993a1a00440392c6c76f39addab8d97c706d2a8bcc2c9b2b6cb2cd7f41df",
+                "sha256:ce1c0580372d3c9bfa151bd0721a9bd5647b9b2245d0588d813fdbd2eb5d6f22",
+                "sha256:d522f28f7b252454df86ac3db5a0e1fe5ae03c8fc0cd1592c912b07c9fad6c29",
+                "sha256:d5d25cc5dad31a10d7a8196125515cc3aa68187c8953459fcaf127c2c8410f51",
+                "sha256:d9f7a692c11de20cb8ec680584815315e03d1404a6e299d36489b0fb6447d98d",
+                "sha256:d9fa2ad4cabb5054faa8d4a44b84134b0ec9d1421f5e9264d057d6be4d13c7fa",
+                "sha256:db53a85f4db0dbd9e5f6277d9153bcaa2ccb87b0d672c6a35f19432b3f2301a3",
+                "sha256:db9d36c4c7997c2a2513a5d218fd90b53bfeaf7e727f94aaf3576973378b3bce",
+                "sha256:e80f02e68d25c222471fcc5d1933275b8eb396e5e40b7863e4e0a43b3c810059",
+                "sha256:e84bd1c29e83ec74a95de070473742eb52d08502f2428eff5751671081e0a0a6",
+                "sha256:f0e12bdafdf7e32c5ad4a073e325ea0d659d4277af8b3d8eccf3101c56879619",
+                "sha256:fd56a9e0c63a1f9c37621fe298c77795aefd2a26dca80dcae27688586c40b4bb"
             ],
             "markers": "python_version >= '2.5' and python_version not in '3.0, 3.1, 3.2, 3.3'",
-            "version": "==3.17.6"
+            "version": "==3.18.0"
         },
         "six": {
             "hashes": [
diff --git a/README.md b/README.md
index b3516b8..9f66f06 100644
--- a/README.md
+++ b/README.md
@@ -11,7 +11,7 @@
 
 ![](example/diagram.png)
 
-[See more example](https://sashimi.readthedocs.io/en/latest/)
+[Tutorials](https://sashimi.readthedocs.io/en/latest/)
 
 ## what is sashimi.py
 
diff --git a/requirements.txt b/requirements.txt
index e7209a0..707ec63 100644
--- a/requirements.txt
+++ b/requirements.txt
@@ -1,51 +1,47 @@
 asciitree>=0.3.3
 cairocffi>=1.4.0
-certifi>=2022.9.24
+certifi>=2022.9.24 ; python_version >= '3.6'
 cffi>=1.15.1
-charset-normalizer>=2.1.1
+charset-normalizer>=2.1.1 ; python_full_version >= '3.6.0'
 click>=8.1.3
-click-option-group>=0.5.3
-contourpy>=1.0.5
+click-option-group>=0.5.5
+contourpy>=1.0.6 ; python_version >= '3.7'
 cooler>=0.8.11
-cycler>=0.11.0
+cycler>=0.11.0 ; python_version >= '3.6'
 cytoolz>=0.10.1
-dill>=0.3.5.1
-filetype>=1.1.0
-fonttools>=4.37.3
-h5py>=3.7.0
+dill>=0.3.6 ; python_version >= '3.7'
+filetype>=1.2.0
+fonttools>=4.38.0 ; python_version >= '3.7'
+h5py>=3.7.0 ; python_version >= '3.7'
 hicmatrix>=15
-idna>=3.4
+idna>=3.4 ; python_version >= '3.5'
 intervaltree>=3.1.0
-kiwisolver>=1.4.4
+kiwisolver>=1.4.4 ; python_version >= '3.7'
 loguru>=0.6.0
-matplotlib>=3.6.0
-multiprocess>=0.70.13
-numexpr>=2.8.3
-numpy>=1.23.3
-packaging>=21.3
-pandas>=1.5.0
-pillow>=9.2.0
+matplotlib>=3.6.2
+multiprocess>=0.70.14 ; python_version >= '3.7'
+numexpr>=2.8.4 ; python_version >= '3.7'
+numpy>=1.23.4
+packaging>=21.3 ; python_version >= '3.6'
+pandas>=1.5.1
+pillow>=9.3.0 ; python_version >= '3.7'
 pybigwig>=0.3.18
 pycparser>=2.21
 pyfaidx>=0.7.1
 pypairix>=0.3.7
-pyparsing>=3.0.9
-pysam>=0.19.1
-python-dateutil>=2.8.2
-pytz>=2022.2.1
-pyyaml>=6.0
+pyparsing>=3.0.9 ; python_full_version >= '3.6.8'
+pysam>=0.20.0
+python-dateutil>=2.8.2 ; python_version >= '2.7' and python_version not in '3.0, 3.1, 3.2, 3.3'
+pytz>=2022.6
+pyyaml>=6.0 ; python_version >= '3.6'
 requests>=2.28.1
-scipy>=1.9.1
-seaborn>=0.12.0
-setuptools>=65.4.0
-simplejson>=3.17.6
-six>=1.16.0
+scipy>=1.9.3
+seaborn>=0.12.1
+setuptools>=65.5.1 ; python_version >= '3.7'
+simplejson>=3.18.0 ; python_version >= '2.5' and python_version not in '3.0, 3.1, 3.2, 3.3'
+six>=1.16.0 ; python_version >= '2.7' and python_version not in '3.0, 3.1, 3.2, 3.3'
 sortedcontainers>=2.4.0
-tables>=3.7.0
-toolz>=0.12.0
-urllib3>=1.26.12
+tables>=3.7.0 ; python_version >= '3.6'
+toolz>=0.12.0 ; python_version >= '3.5'
+urllib3>=1.26.12 ; python_version >= '2.7' and python_version not in '3.0, 3.1, 3.2, 3.3, 3.4, 3.5' and python_version < '4'
 xmltodict>=0.13.0
-
-uvicorn~=0.18.2
-fastapi~=0.79.0
-pydantic~=1.9.1
\ No newline at end of file
diff --git a/sashimi/base/ReadDepth.py b/sashimi/base/ReadDepth.py
index 63a92d1..18f4ae1 100644
--- a/sashimi/base/ReadDepth.py
+++ b/sashimi/base/ReadDepth.py
@@ -47,7 +47,6 @@ def __init__(self,
         self.junctions_dict = junctions_dict
         self.strand_aware = strand_aware
         self.minus = abs(minus) if minus is not None else minus
-        self.max = max(self.wiggle, default=0)
         self.junction_dict_plus = junction_dict_plus
         self.junction_dict_minus = junction_dict_minus
         self.site_plus = site_plus
@@ -55,7 +54,7 @@ def __init__(self,
 
     @property
     def wiggle(self) -> np.array:
-        if (self.plus is None or np.sum(self.plus) == 0) and self.minus is not None:
+        if (self.plus is None or not self.plus.any()) and self.minus is not None:
             return self.minus
 
         if self.plus is not None and self.minus is not None:
@@ -63,6 +62,10 @@ def wiggle(self) -> np.array:
 
         return self.plus
 
+    @property
+    def max(self) -> float:
+        return max(self.wiggle, default=0)
+
     def __add__(self, other):
 
         """
@@ -74,28 +77,33 @@ def __add__(self, other):
                 A new ReadDepth object containing the sum of the two original ReadDepth objects
         """
 
-        if len(self.wiggle) == len(other.wiggle):
-            junctions = self.junctions_dict if self.junctions_dict else {}
-            if other.junctions_dict:
-                for i, j in other.junctions_dict.items():
-                    if i in junctions.keys():
-                        junctions[i] += j
-                    else:
-                        junctions[i] = j
-
-            minus = None
-            if self.minus is not None and other.minus is not None:
-                minus = self.minus + other.minus
-            elif self.minus is None and other.minus is not None:
-                minus = other.minus
-            elif self.minus is not None and other.minus is None:
-                minus = self.minus
-
-            return ReadDepth(
-                self.plus + other.plus,
-                junctions_dict=junctions,
-                minus=minus
-            )
+        if self.wiggle is not None and other.wiggle is not None:
+            if len(self.wiggle) == len(other.wiggle):
+                junctions = self.junctions_dict if self.junctions_dict else {}
+                if other.junctions_dict:
+                    for i, j in other.junctions_dict.items():
+                        if i in junctions.keys():
+                            junctions[i] += j
+                        else:
+                            junctions[i] = j
+
+                minus = None
+                if self.minus is not None and other.minus is not None:
+                    minus = self.minus + other.minus
+                elif self.minus is None and other.minus is not None:
+                    minus = other.minus
+                elif self.minus is not None and other.minus is None:
+                    minus = self.minus
+
+                return ReadDepth(
+                    self.plus + other.plus,
+                    junctions_dict=junctions,
+                    minus=minus
+                )
+        elif self.wiggle is None:
+            return other
+        else:
+            return self
 
     def curr_height(self, pos: int) -> float:
         if self.minus is None:
diff --git a/sashimi/cli.py b/sashimi/cli.py
index d6a4850..bee9c0f 100644
--- a/sashimi/cli.py
+++ b/sashimi/cli.py
@@ -21,7 +21,7 @@
 from sashimi.file.ATAC import ATAC
 from sashimi.plot import Plot
 
-__version__ = "0.0.6"
+__version__ = "0.0.7"
 __author__ = "ygidtu & Ran Zhou"
 __email__ = "ygidtu@gmail.com"
 
@@ -49,6 +49,9 @@ def __init__(self,
                  library: str = "fru",
                  trans: Optional[str] = None,
                  depth: int = 30000):
+
+        if path.startswith("~"):
+            path = os.path.expanduser(path)
         self.path = os.path.abspath(path)
 
         if not os.path.exists(self.path):
@@ -63,6 +66,10 @@ def __init__(self,
         self.trans = trans
         self.depth = depth
 
+    @property
+    def name(self) -> str:
+        return os.path.basename(self.path)
+
     def __str__(self):
         return f"path: {self.path} \nlabel: {self.label} \ngroup: {self.group} \n" \
                f"color: {self.color} \ncategory: {self.category} \nlibrary: {self.library}"
@@ -114,7 +121,7 @@ def __read_iter__(path):
 
 def process_file_list(infile: str, category: str = "density"):
     u"""
-    Process and check the file list format
+    Process and check the file list format_
     :param infile: path to input file list
     :param category: the image type of file list used for
     """
@@ -235,7 +242,7 @@ def process_file_list(infile: str, category: str = "density"):
                  show_default=True)
 @optgroup.option("--barcode", type=click.Path(exists=True), show_default=True,
                  help="Path to barcode list file, At list two columns were required, "
-                      "- 1st The name of bam file; \b"
+                      "- 1st The name of bam file, not the alias of bam; \b"
                       "- 2nd the barcode; \b"
                       "- 3rd The group label, optional; \b"
                       "- 4th The color of each cell type, default using the color of corresponding bam file.\n")
@@ -519,8 +526,8 @@ def main(**kwargs):
                     p.add_interval(f.path, f.label)
             elif key == "density":
                 for f in process_file_list(kwargs[key], key):
-                    if barcodes and f.label in barcodes.keys() and f.category in ["bam", "atac"]:
-                        for group in barcodes[f.label].keys():
+                    if barcodes and f.name in barcodes.keys() and f.category in ["bam", "atac"]:
+                        for group in barcodes[f.name].keys():
                             if kwargs["group_by_cell"] and group:
                                 label = group
                             elif group:
@@ -530,13 +537,13 @@ def main(**kwargs):
 
                             if f.label not in size_factors.keys() and f.category == "atac":
                                 logger.info(f"Indexing {f.path}")
-                                size_factors[f.label] = ATAC.index(f.path, barcodes[f.label])
+                                size_factors[f.label] = ATAC.index(f.path, barcodes[f.name])
 
                             p.add_density(f.path,
                                           category=f.category,
                                           label=label,
                                           barcode=group,
-                                          barcode_groups=barcodes[f.label],
+                                          barcode_groups=barcodes[f.name],
                                           barcode_tag=kwargs["barcode_tag"],
                                           umi_tag=kwargs["umi_tag"],
                                           library=f.library,
@@ -568,17 +575,17 @@ def main(**kwargs):
                                       density_by_strand=kwargs["density_by_strand"],)
             elif key == "heatmap":
                 for f in process_file_list(kwargs[key], key):
-                    if barcodes and f.label in barcodes.keys() and f.category in ["bam", "atac"]:
+                    if barcodes and f.name in barcodes.keys() and f.category in ["bam", "atac"]:
                         if f.label not in size_factors.keys() and f.category == "atac":
                             logger.info(f"Indexing {f.path}")
-                            size_factors[f.label] = ATAC.index(f.path, barcodes[f.label])
+                            size_factors[f.label] = ATAC.index(f.path, barcodes[f.name])
 
-                        for group in barcodes[f.label].keys():
+                        for group in barcodes[f.name].keys():
                             p.add_heatmap(f.path,
                                           category=f.category,
                                           label=f"{f.label} - {group}" if group else f.label,
                                           barcode=group,
-                                          barcode_groups=barcodes[f.label],
+                                          barcode_groups=barcodes[f.name],
                                           group=f"{f.group} - {group}" if f.group else f.group,
                                           barcode_tag=kwargs["barcode_tag"],
                                           size_factor=size_factors.get(f.label),
@@ -613,8 +620,8 @@ def main(**kwargs):
                                       vmax=kwargs["heatmap_vmax"])
             elif key == "line":
                 for f in process_file_list(kwargs[key], key):
-                    if barcodes and f.label in barcodes.keys() and f.category == "bam":
-                        for group in barcodes[f.label].keys():
+                    if barcodes and f.name in barcodes.keys() and f.category == "bam":
+                        for group in barcodes[f.name].keys():
                             if kwargs["group_by_cell"] and group:
                                 label = group
                             elif group:
@@ -732,7 +739,8 @@ def main(**kwargs):
             "density": kwargs["sc_density_height_ratio"]
         },
         distance_between_label_axis=kwargs["distance_ratio"],
-        included_junctions=included_junctions
+        included_junctions=included_junctions,
+        n_jobs=kwargs.get("process", 1)
     )
 
 
diff --git a/sashimi/file/File.py b/sashimi/file/File.py
index a9f8055..991893e 100644
--- a/sashimi/file/File.py
+++ b/sashimi/file/File.py
@@ -38,6 +38,9 @@ def end(self) -> int:
     def load(self, *args, **kwargs):
         return None
 
+    def len(self, scale=1) -> int:
+        return len(self.data) / scale if self.data else 0
+
     def __hash__(self) -> int:
         return hash((self.path, self.label, self.title))
 
diff --git a/sashimi/file/Reference.py b/sashimi/file/Reference.py
index 5d4f4e9..9fd967b 100644
--- a/sashimi/file/Reference.py
+++ b/sashimi/file/Reference.py
@@ -13,7 +13,6 @@
 from typing import List, Union, Optional
 
 import filetype
-import matplotlib as mpl
 import pysam
 from loguru import logger
 
@@ -22,7 +21,6 @@
 from sashimi.base.Readder import Reader
 from sashimi.base.Transcript import Transcript
 from sashimi.file.File import File
-from sashimi.base.CoordinateMap import Coordinate
 
 
 class Reference(File):
@@ -133,9 +131,7 @@ def __load_local_domain__(self, region: GenomicLoci):
                 for record in Reader.read_bigbed(bb_file, region):
                     record = record[2].split("\t")
                     current_id = record[0]
-                    strand = record[2]
                     current_start = int(record[3])
-                    num_of_chunk = record[6]
                     block_sizes = [int(x) for x in record[7].split(",") if x]
                     block_starts = [int(x) for x in record[8].split(",") if x]
                     current_desc = record[17]
@@ -307,7 +303,6 @@ def index_gtf(cls, input_gtf):
         gtf = cls.is_gtf(input_gtf)
         assert gtf % 10 == 1, f"{input_gtf} seems not be gtf format"
 
-        index = False
         if gtf // 10 > 0:
             output_gtf = input_gtf
         else:
@@ -369,11 +364,10 @@ def __load_gtf__(self, region: GenomicLoci) -> List[Transcript]:
                         strand=rec.strand,
                         transcript_id=rec.transcript_id,
                         gene_id=rec.gene_id,
-                        gene=rec.gene_name,
-                        transcript=rec.transcript_name,
+                        gene=rec.gene_name if "gene_name" in rec.attributes else "",
+                        transcript=rec.transcript_name if "transcript_name" in rec.attributes else "",
                         exons=[]
                     )
-
             elif re.search(r"(exon)", rec.feature, re.I):
                 if rec.transcript_id not in exons.keys():
                     exons[rec.transcript_id] = []
@@ -444,12 +438,11 @@ def __load_bam__(self, region: GenomicLoci, threshold_of_reads: int = 0) -> List
 
         return sorted([x for x, y in transcripts.items() if y > threshold_of_reads])
 
-    def __load_bed__(self, region: GenomicLoci)-> List[Transcript]:
+    def __load_bed__(self, region: GenomicLoci) -> List[Transcript]:
         transcripts = []
         try:
             for rec in Reader.read_gtf(self.path, region=region, bed=True):
                 exon_bound = []
-                intron_bound = []
                 current_start = int(rec[1])
                 current_end = int(rec[2])
                 if len(rec) > 3:
@@ -525,6 +518,7 @@ def load(self, region: GenomicLoci, threshold_of_reads: int = 0, **kwargs):
         elif self.category == "bed":
             self.data = self.__load_bed__(region)
 
+        rec, start, end, strand = None, None, None, None
         for interval_file, interval_label in self.interval_file.items():
             try:
                 if not os.path.exists(interval_file + ".tbi"):
@@ -562,7 +556,7 @@ def load(self, region: GenomicLoci, threshold_of_reads: int = 0, **kwargs):
                         )
                     )
 
-                if len(interval_target) != 0:
+                if len(interval_target) != 0 and rec is not None:
                     self.data.append(Transcript(
                         chromosome=rec.contig,
                         start=start,
diff --git a/sashimi/plot.py b/sashimi/plot.py
index bddea90..3f97608 100644
--- a/sashimi/plot.py
+++ b/sashimi/plot.py
@@ -5,7 +5,7 @@
 """
 import io
 import logging
-import os.path
+import os
 from typing import Set
 
 import matplotlib.pyplot as plt
@@ -381,6 +381,7 @@ def __init_input_file__(path: str,
                             # for ATAC
                             size_factor=None
                             ):
+        path = os.path.expanduser(path)
         if category == "bam":
             obj = Bam.create(
                 path,
@@ -597,8 +598,10 @@ def add_heatmap(self,
                     'pink', 'spring', 'summer', 'autumn', 'winter', 'cool',
                     'Wistia', 'hot', 'afmhot', 'gist_heat', 'copper'
         :param show_row_names:
-        :param vmin: Values to anchor the colormap, otherwise they are inferred from the data and other keyword arguments.
-        :param vmax: Values to anchor the colormap, otherwise they are inferred from the data and other keyword arguments.
+        :param vmin: Values to anchor the colormap,
+                     otherwise they are inferred from the data and other keyword arguments.
+        :param vmax: Values to anchor the colormap,
+                     otherwise they are inferred from the data and other keyword arguments.
         :return:
         """
         obj, category = self.__init_input_file__(
@@ -923,6 +926,7 @@ def plot(self,
              return_image: Optional[str] = None,
              sc_height_ratio: Optional[Dict[str, float]] = None,
              distance_between_label_axis: float = .3,
+             n_jobs: int = 1,
              *args, **kwargs):
         u"""
         draw image
@@ -935,6 +939,7 @@ def plot(self,
         :param raster: plot rasterizer site plot
         :param sc_height_ratio: adjust the relative height of single cell plots
         :param distance_between_label_axis: distance between y-axis label and y-axis ticks
+        :param n_jobs: load data in how many processes
         :param return_image: used for interactive ui
         """
         if sc_height_ratio is None:
@@ -965,7 +970,8 @@ def plot(self,
             assert isinstance(p, PlotInfo), f"unrecognized data type: {type(p)}"
             try:
                 p.load(region=self.region if p.type != "motif" else self.params[p]["region"],
-                       junctions=self.junctions.get(p.obj[0].label, {}), *args, **kwargs)
+                       junctions=self.junctions.get(p.obj[0].label, {}),
+                       n_jobs=n_jobs, *args, **kwargs)
             except Exception as err:
                 logger.warning(f"failed to load data from {p}")
                 raise err
diff --git a/server.py b/server.py
index 36869ac..b69c5f8 100644
--- a/server.py
+++ b/server.py
@@ -5,7 +5,6 @@
 """
 
 import inspect
-import os
 import pickle
 import sys
 import traceback
diff --git a/setup.py b/setup.py
index 2b42abb..a114101 100644
--- a/setup.py
+++ b/setup.py
@@ -1,7 +1,7 @@
 import os
 from setuptools import setup, find_packages
 
-__version__ = "0.0.6"
+__version__ = "0.0.7"
 __author__ = "ygidtu & Ran Zhou"
 __email__ = "ygidtu@gmail.com"
 
diff --git a/web/package.json b/web/package.json
index 2e051d8..aaf660b 100644
--- a/web/package.json
+++ b/web/package.json
@@ -1,7 +1,7 @@
 {
   "name": "sashimi.py",
   "private": true,
-  "version": "0.0.6",
+  "version": "0.0.7",
   "type": "module",
   "scripts": {
     "dev": "vite",