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Scaffolding with parental reads #28

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DustinSokolowski opened this issue Mar 26, 2024 · 0 comments
Open

Scaffolding with parental reads #28

DustinSokolowski opened this issue Mar 26, 2024 · 0 comments

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@DustinSokolowski
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Hello and thank you for the excellent tool.

I have a PB + nanopore assembly and 10X linked reads from the parents for trio binning, however I do not have the linked reads for the individual with PB and nanopore (I have it for his littermate -- long story). Would you recommend combining the paternal linked reads into a single fastq file (i.e., cat maternal_R1.fastq.gz paternal_R1_fastq.gz ; cat maternal_R2.fastq.gz paternal_R2_fastq.gz ) and running scaff10X on these merged data, or could you see this leading to misassemblies?

Thanks,
Dustin

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