diff --git a/.changeset/weak-falcons-drop.md b/.changeset/weak-falcons-drop.md
new file mode 100644
index 00000000000..08d7e092869
--- /dev/null
+++ b/.changeset/weak-falcons-drop.md
@@ -0,0 +1,5 @@
+---
+"@astrojs/starlight": patch
+---
+
+Adds icon support for `.otf` files in `<FileTree>`
diff --git a/packages/starlight/user-components/file-tree-icons.ts b/packages/starlight/user-components/file-tree-icons.ts
index a62a37d59da..d8c6af38088 100644
--- a/packages/starlight/user-components/file-tree-icons.ts
+++ b/packages/starlight/user-components/file-tree-icons.ts
@@ -340,6 +340,7 @@ export const definitions: Definitions = {
 		'.ttf': 'seti:font',
 		'.woff': 'seti:font',
 		'.woff2': 'seti:font',
+		'.otf': 'seti:font',
 		'.avif': 'seti:image',
 		'.gif': 'seti:image',
 		'.jpg': 'seti:image',