diff --git a/.changeset/ten-sloths-invent.md b/.changeset/ten-sloths-invent.md
new file mode 100644
index 000000000000..12c5bdf5fe2e
--- /dev/null
+++ b/.changeset/ten-sloths-invent.md
@@ -0,0 +1,7 @@
+---
+'astro': patch
+---
+
+Use UInt8Array instead of Buffer for both the input and return values of the `transform()` hook of the Image Service API to ensure compatibility with non-Node runtimes.
+
+This change is unlikely to affect you, but if you were previously relying on the return value being a Buffer, you may convert an `UInt8Array` to a `Buffer` using `Buffer.from(your_array)`.
diff --git a/packages/astro/src/assets/build/generate.ts b/packages/astro/src/assets/build/generate.ts
index c34e136ddd52..be637c26da7a 100644
--- a/packages/astro/src/assets/build/generate.ts
+++ b/packages/astro/src/assets/build/generate.ts
@@ -41,7 +41,7 @@ type AssetEnv = {
 	assetsFolder: AstroConfig['build']['assets'];
 };
 
-type ImageData = { data: Buffer; expires: number };
+type ImageData = { data: Uint8Array; expires: number };
 
 export async function prepareAssetsGenerationEnv(
 	pipeline: BuildPipeline,
diff --git a/packages/astro/src/assets/endpoint/generic.ts b/packages/astro/src/assets/endpoint/generic.ts
index 140189fe088c..a158448edbcf 100644
--- a/packages/astro/src/assets/endpoint/generic.ts
+++ b/packages/astro/src/assets/endpoint/generic.ts
@@ -14,7 +14,7 @@ async function loadRemoteImage(src: URL) {
 			return undefined;
 		}
 
-		return Buffer.from(await res.arrayBuffer());
+		return await res.arrayBuffer();
 	} catch (err: unknown) {
 		return undefined;
 	}
@@ -38,7 +38,7 @@ export const GET: APIRoute = async ({ request }) => {
 			throw new Error('Incorrect transform returned by `parseURL`');
 		}
 
-		let inputBuffer: Buffer | undefined = undefined;
+		let inputBuffer: ArrayBuffer | undefined = undefined;
 
 		const sourceUrl = isRemotePath(transform.src)
 			? new URL(transform.src)
@@ -54,7 +54,11 @@ export const GET: APIRoute = async ({ request }) => {
 			return new Response('Not Found', { status: 404 });
 		}
 
-		const { data, format } = await imageService.transform(inputBuffer, transform, imageConfig);
+		const { data, format } = await imageService.transform(
+			new Uint8Array(inputBuffer),
+			transform,
+			imageConfig
+		);
 
 		return new Response(data, {
 			status: 200,
diff --git a/packages/astro/src/assets/services/service.ts b/packages/astro/src/assets/services/service.ts
index 5a063d4670ae..ab647b7107a0 100644
--- a/packages/astro/src/assets/services/service.ts
+++ b/packages/astro/src/assets/services/service.ts
@@ -96,10 +96,10 @@ export interface LocalImageService<T extends Record<string, any> = Record<string
 	 * final image format of the optimized image.
 	 */
 	transform: (
-		inputBuffer: Buffer,
+		inputBuffer: Uint8Array,
 		transform: LocalImageTransform,
 		imageConfig: ImageConfig<T>
-	) => Promise<{ data: Buffer; format: ImageOutputFormat }>;
+	) => Promise<{ data: Uint8Array; format: ImageOutputFormat }>;
 
 	/**
 	 * A list of properties that should be used to generate the hash for the image.
diff --git a/packages/astro/src/assets/services/squoosh.ts b/packages/astro/src/assets/services/squoosh.ts
index 5be5d40770c6..c9586af10d6c 100644
--- a/packages/astro/src/assets/services/squoosh.ts
+++ b/packages/astro/src/assets/services/squoosh.ts
@@ -30,7 +30,7 @@ const qualityTable: Record<
 };
 
 async function getRotationForEXIF(
-	inputBuffer: Buffer,
+	inputBuffer: Uint8Array,
 	src?: string
 ): Promise<Operation | undefined> {
 	const meta = await imageMetadata(inputBuffer, src);
diff --git a/packages/astro/src/assets/services/vendor/squoosh/image-pool.ts b/packages/astro/src/assets/services/vendor/squoosh/image-pool.ts
index 839708f03b41..cc2df9c96ffe 100644
--- a/packages/astro/src/assets/services/vendor/squoosh/image-pool.ts
+++ b/packages/astro/src/assets/services/vendor/squoosh/image-pool.ts
@@ -19,7 +19,7 @@ const getWorker = execOnce(() => {
 
 type DecodeParams = {
 	operation: 'decode';
-	buffer: Buffer;
+	buffer: Uint8Array;
 };
 type ResizeParams = {
 	operation: 'resize';
@@ -86,7 +86,7 @@ function handleJob(params: JobMessage) {
 }
 
 export async function processBuffer(
-	buffer: Buffer,
+	buffer: Uint8Array,
 	operations: Operation[],
 	encoding: ImageOutputFormat,
 	quality?: number
diff --git a/packages/astro/src/assets/utils/metadata.ts b/packages/astro/src/assets/utils/metadata.ts
index 2ee96a7aca28..175bacc050e7 100644
--- a/packages/astro/src/assets/utils/metadata.ts
+++ b/packages/astro/src/assets/utils/metadata.ts
@@ -3,9 +3,10 @@ import { AstroError, AstroErrorData } from '../../core/errors/index.js';
 import type { ImageInputFormat, ImageMetadata } from '../types.js';
 
 export async function imageMetadata(
-	data: Buffer,
+	data: Uint8Array,
 	src?: string
 ): Promise<Omit<ImageMetadata, 'src' | 'fsPath'>> {
+	// @ts-expect-error probe-image-size types are wrong, it does accept Uint8Array. From the README: "Sync version can eat arrays, typed arrays and buffers."
 	const result = probe.sync(data);
 
 	if (result === null) {