Parameter Value Version 2.6.3.0 User name animeshs Machine name DMED7596 Date of writing 07/30/2024 22:13:46 Include contaminants True PSM FDR 0.01 PSM FDR Crosslink 0.01 Protein FDR 0.01 Site FDR 0.01 Use Normalized Ratios For Occupancy True Min. peptide Length 7 Min. score for unmodified peptides 0 Min. score for modified peptides 40 Min. delta score for unmodified peptides 0 Min. delta score for modified peptides 6 Min. unique peptides 1 Min. razor peptides 1 Min. peptides 1 Use only unmodified peptides and True Modifications included in protein quantification Oxidation (M);Acetyl (Protein N-term) Peptides used for protein quantification Unique Discard unmodified counterpart peptides True Label min. ratio count 1 Use delta score False iBAQ True iBAQ log fit True Match between runs False Find dependent peptides False Fasta file Z:\uniprotkb_proteome_UP000005640_2024_07_22.fasta Decoy mode revert Include contaminants True Advanced ratios True Fixed andromeda index folder Second peptides True Stabilize large LFQ ratios True Separate LFQ in parameter groups False Require MS/MS for LFQ comparisons True Calculate peak properties False Main search max. combinations 200 Advanced site intensities True Write msScans table False Write msmsScans table True Write ms3Scans table True Write allPeptides table False Write mzRange table True Write DIA fragments table False Write DIA fragments quant table False Write pasefMsmsScans table True Write accumulatedMsmsScans table True Max. peptide mass [Da] 4600 Min. peptide length for unspecific search 8 Max. peptide length for unspecific search 25 Razor protein FDR True Disable MD5 False Max mods in site table 3 Match unidentified features False Epsilon score for mutations True Evaluate variant peptides separately True Variation mode None MS/MS tol. (FTMS) 20 ppm Top MS/MS peaks per Da interval. (FTMS) 12 Da interval. (FTMS) 100 MS/MS deisotoping (FTMS) True MS/MS deisotoping tolerance (FTMS) 7 MS/MS deisotoping tolerance unit (FTMS) ppm MS/MS higher charges (FTMS) True MS/MS water loss (FTMS) True MS/MS water loss (FTMS for cross link) False MS/MS ammonia loss (FTMS) True MS/MS ammonia loss (FTMS for cross link) False MS/MS dependent losses (FTMS) True MS/MS recalibration (FTMS) False MS/MS tol. (ITMS) 0.5 Da Top MS/MS peaks per Da interval. (ITMS) 8 Da interval. (ITMS) 100 MS/MS deisotoping (ITMS) False MS/MS deisotoping tolerance (ITMS) 0.15 MS/MS deisotoping tolerance unit (ITMS) Da MS/MS higher charges (ITMS) True MS/MS water loss (ITMS) True MS/MS water loss (ITMS for cross link) False MS/MS ammonia loss (ITMS) True MS/MS ammonia loss (ITMS for cross link) False MS/MS dependent losses (ITMS) True MS/MS recalibration (ITMS) False MS/MS tol. (TOF) 25 ppm Top MS/MS peaks per Da interval. (TOF) 16 Da interval. (TOF) 100 MS/MS deisotoping (TOF) True MS/MS deisotoping tolerance (TOF) 0.01 MS/MS deisotoping tolerance unit (TOF) Da MS/MS higher charges (TOF) True MS/MS water loss (TOF) True MS/MS water loss (TOF for cross link) False MS/MS ammonia loss (TOF) True MS/MS ammonia loss (TOF for cross link) False MS/MS dependent losses (TOF) True MS/MS recalibration (TOF) False MS/MS tol. (UNKNOWN) 20 ppm Top MS/MS peaks per Da interval. (UNKNOWN) 12 Da interval. (UNKNOWN) 100 MS/MS deisotoping (UNKNOWN) True MS/MS deisotoping tolerance (UNKNOWN) 7 MS/MS deisotoping tolerance unit (UNKNOWN) ppm MS/MS higher charges (UNKNOWN) True MS/MS water loss (UNKNOWN) True MS/MS water loss (UNKNOWN for cross link) False MS/MS ammonia loss (UNKNOWN) True MS/MS ammonia loss (UNKNOWN for cross link) False MS/MS dependent losses (UNKNOWN) True MS/MS recalibration (UNKNOWN) False Site tables