gsutil -q -o GSUtil:parallel_composite_upload_threshold=150M -m rsync -d -r gs://fc-secure-5a278fa4-ea59-4086-be31-07b6d217ec5e/CITESeq-Sequencing/060724_DP1_Batch_2_3_NS2000_Pool/FASTQs/IFNB_B3_adt IFNB_B3_adt_0 gsutil -q -o GSUtil:parallel_composite_upload_threshold=150M -m rsync -d -r gs://fc-secure-5a278fa4-ea59-4086-be31-07b6d217ec5e/CITESeq-Sequencing/062124_DP1_Batch_2_3_NOVA/062724_fastqs/IFNB_B3_adt IFNB_B3_adt_1 Load feature barcodes. /cromwell_root/fc-secure-5a278fa4-ea59-4086-be31-07b6d217ec5e/101723_NS2000_DP1/101723_NS2000_ADT_HTO.csv is parsed. n_barcodes = 148, and barcode_len = 15. In the index, 18 out of 257650 items are ambigious, percentage = 0.01%. ntotA = 5, ntotBC = 9604. TotalSeq type is automatically detected as TotalSeq-C, barcode starts from 0-based position 10. Load cell barcodes. /cromwell_root/regev-lab/resources/cellranger/3M-3pgex-may-2023.txt.gz is parsed. n_barcodes = 7372800, and barcode_len = 16. In the index, 82135371 out of 295849913 items are ambigious, percentage = 27.76%. Time spent on parsing cell barcodes = 281.00s. Processed 1000000 reads. Processed 2000000 reads. Processed 3000000 reads. Processed 4000000 reads. Processed 5000000 reads. Processed 6000000 reads. Processed 7000000 reads. Processed 8000000 reads. Processed 9000000 reads. Processed 10000000 reads. Processed 11000000 reads. Processed 12000000 reads. Processed 13000000 reads. Processed 14000000 reads. Processed 15000000 reads. Processed 16000000 reads. Processed 17000000 reads. Processed 18000000 reads. Processed 19000000 reads. Processed 20000000 reads. Processed 21000000 reads. Processed 22000000 reads. Processed 23000000 reads. Processed 24000000 reads. Processed 25000000 reads. Processed 26000000 reads. Processed 27000000 reads. Processed 28000000 reads. Processed 29000000 reads. Processed 30000000 reads. Processed 31000000 reads. Processed 32000000 reads. Processed 33000000 reads. 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Total time spent (not including destruct objects) = 2385.00s. strato exists gs://fc-secure-5a278fa4-ea59-4086-be31-07b6d217ec5e/CITESeq-Sequencing/060724_DP1_Batch_2_3_NS2000_Pool/FASTQs/IFNB_B3_adt/ strato sync -m gs://fc-secure-5a278fa4-ea59-4086-be31-07b6d217ec5e/CITESeq-Sequencing/060724_DP1_Batch_2_3_NS2000_Pool/FASTQs/IFNB_B3_adt IFNB_B3_adt_0 strato exists gs://fc-secure-5a278fa4-ea59-4086-be31-07b6d217ec5e/CITESeq-Sequencing/062124_DP1_Batch_2_3_NOVA/062724_fastqs/IFNB_B3_adt/ strato sync -m gs://fc-secure-5a278fa4-ea59-4086-be31-07b6d217ec5e/CITESeq-Sequencing/062124_DP1_Batch_2_3_NOVA/062724_fastqs/IFNB_B3_adt IFNB_B3_adt_1 generate_count_matrix_ADTs /cromwell_root/regev-lab/resources/cellranger/3M-3pgex-may-2023.txt.gz /cromwell_root/fc-secure-5a278fa4-ea59-4086-be31-07b6d217ec5e/101723_NS2000_DP1/101723_NS2000_ADT_HTO.csv IFNB_B3_adt_0,IFNB_B3_adt_1 IFNB_B3_adt -p 4 --max-mismatch-feature 2 --feature antibody --max-mismatch-cell 1 --umi-length 10 gsutil -q -o GSUtil:parallel_composite_upload_threshold=150M -m cp IFNB_B3_adt.csv IFNB_B3_adt.stat.csv.gz IFNB_B3_adt.report.txt gs://fc-secure-5a278fa4-ea59-4086-be31-07b6d217ec5e/CITESeq-Sequencing/062124_DP1_Batch_2_3_NOVA/070324_cellranger_output/IFNB_B3_adt/