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There was an error in the function 'prep_SRST' #2

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khoriba opened this issue Mar 22, 2022 · 0 comments
Open

There was an error in the function 'prep_SRST' #2

khoriba opened this issue Mar 22, 2022 · 0 comments

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@khoriba
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khoriba commented Mar 22, 2022

Hi,

I encountered this error. What can I do to correct this? Your help is needed.

$ emm_typing.py -m EMM_data/ -1 R1.fq -2 R2.fq -o AC/
There was an error in the function 'prep_SRST'
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Traceback (most recent call last):
  File "/Users/home/local/emm-typing-tool/modules/utility_functions.py", line 54, in try_and_except
    return function(*parameters, **named_parameters)
  File "/Users/home/local/emm-typing-tool/modules/EMM_determiner_functions.py", line 137, in prep_SRST
    stdout_log_output, stderr_log_output = cline()
  File "/Users/home/anaconda3/envs/gbs/lib/python2.7/site-packages/Bio/Application/__init__.py", line 531, in __call__
    stdout_str, stderr_str)
ApplicationError: Non-zero return code 1 from 'blastn -out ../ACC5414A1//my_blast_tmp.xml -outfmt 5 -query AC/emm_tsdna_bait.fasta -db EMM_data/reference -evalue 0.001', message 'Command line argument error: Argument "query". File is not accessible:  `AC/emm_tsdna_bait.fasta\''
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