diff --git a/plugin/trino-hive/src/main/java/io/trino/plugin/hive/metastore/glue/GlueMetastoreModule.java b/plugin/trino-hive/src/main/java/io/trino/plugin/hive/metastore/glue/GlueMetastoreModule.java
index 2217c7690d49..ca70fea9ddc7 100644
--- a/plugin/trino-hive/src/main/java/io/trino/plugin/hive/metastore/glue/GlueMetastoreModule.java
+++ b/plugin/trino-hive/src/main/java/io/trino/plugin/hive/metastore/glue/GlueMetastoreModule.java
@@ -46,6 +46,7 @@
 import java.util.Set;
 
 import static com.google.common.base.Preconditions.checkArgument;
+import static com.google.common.base.Preconditions.checkState;
 import static com.google.inject.multibindings.OptionalBinder.newOptionalBinder;
 import static com.google.inject.multibindings.ProvidesIntoOptional.Type.DEFAULT;
 import static io.airlift.configuration.ConfigBinder.configBinder;
@@ -96,6 +97,12 @@ public static GlueCache createGlueCache(CachingHiveMetastoreConfig config, NodeM
         boolean enabled = nodeManager.getCurrentNode().isCoordinator() &&
                 (metadataCacheTtl.toMillis() > 0 || statsCacheTtl.toMillis() > 0);
 
+        checkState(config.getMetastoreRefreshInterval().isEmpty(), "Metastore refresh interval is not supported with Glue v2");
+        checkState(config.isPartitionCacheEnabled(), "Disabling partitions cache is not supported with Glue v2");
+        checkState(config.isCacheMissing(), "Disabling cache missing is not supported with Glue v2");
+        checkState(config.isCacheMissingPartitions(), "Disabling cache missing partitions is not supported with Glue v2");
+        checkState(config.isCacheMissingStats(), "Disabling cache missing stats is not supported with Glue v2");
+
         if (enabled) {
             return new InMemoryGlueCache(metadataCacheTtl, statsCacheTtl, config.getMetastoreCacheMaximumSize());
         }