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improve dynamic range of fluoresence sensing #1

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astraw opened this issue Oct 23, 2020 · 2 comments
Open

improve dynamic range of fluoresence sensing #1

astraw opened this issue Oct 23, 2020 · 2 comments
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enhancement New feature or request

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@astraw
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astraw commented Oct 23, 2020

Here's the result of an experiment we just ran. As can be seen, some the fluorescence values are saturated. This is likely from exceeding the dynamic range of the sensor as we are using it. It would be good if they didn't saturate.

Image Pasted at 2020-10-23 12-25

IMG_4348

@astraw
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astraw commented Oct 28, 2020

Perhaps reducing the gain by a factor of 2 would be sufficient.

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astraw commented Dec 7, 2020

It might be possible to obtain a non-saturated reading of highly fluorescent wells by pulsing the LEDs very briefly. (Either as a single pulse or a low duty cycle on a PWM LED illumination program which the ADC and photodiode integrate over.)

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