scRNA.sub =subset(scRNAnew, downsample=30)

[seekning]

scRNA.sub =subset(scRNAnew, downsample=30) ，argument "downsample" didn't work,what ‘s wrong?

Idents(scRNAnew) = "celltype"
scRNA.sub =subset(scRNAnew, downsample=30) # downsample函数实现分层抽样 每个细胞类型抽200个
scRNA.sub
An object of class Seurat
29984 features across 300 samples within 2 assays
Active assay: RNA (27984 features, 0 variable features)
1 other assay present: integrated
3 dimensional reductions calculated: pca, umap, tsne
table(scRNA.sub$celltype)

  Fibroblasts          NK_cells           T_cells  Epithelial_cells       Macrophages 
           89                88                23                31                25 
    Monocytes      Plasma_cells               SMC Endothelial_cells           B_cells 
           13                10                 8                11                 2 

[rharao]

Interesting. Thanks for posting. Could you provide the results of sessionInfo() and table(scRNAnew$celltype)?