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import-and-align.bsh
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import-and-align.bsh
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/**
* Call
*
* xvfb-run -a ./ImageJ-linux64 -Ddir=<project directory> -Dfile=<import file> -- --no-splash import-and-align.bsh
*
* on the cluster with absolute paths, e.g.
*
* ${HOME}/bin/xvfb-run -a ${HOME}/packages/Fiji.app/fiji-linux64 \
* -Xms10g -Xmx10g -Ddir=${HOME}/AL-FIB-Z0712-12/0-99 \
* -Dfile=${HOME}/AL-FIB-Z0712-12/0-99/import.txt -- --no-splash \
* ${HOME}/AL-FIB-Z0712-12/AL-FIB-Z0712-12/import-and-align.bsh
*
* @author Stephan Saalfeld <[email protected]>
*/
import ini.trakem2.ControlWindow;
import ini.trakem2.Project;
import ini.trakem2.display.Layer;
import ini.trakem2.display.Patch;
import ini.trakem2.utils.Filter;
import ij.ImagePlus;
import java.lang.Runtime;
import java.util.ArrayList;
import java.util.HashSet;
import mpicbg.trakem2.align.ElasticLayerAlignment;
import mpicbg.trakem2.align.RegularizedAffineLayerAlignment;
try {
runtime = Runtime.getRuntime();
System.out.println( runtime.availableProcessors() + " cores available for multi-threading" );
dir = System.getProperty("dir");
file = System.getProperty("file");
ControlWindow.setGUIEnabled(false);
project = Project.newFSProject("blank", null, dir, false);
loader = project.getLoader();
layerset = project.getRootLayerSet();
layerset.setSnapshotsMode(1);
/* add a reference layer (pointless gymnastik) */
layer = new Layer(project, 0, 1, layerset);
layerset.add(layer);
layer.recreateBuckets();
/* import images */
task = loader.importImages(
layerset.getLayer(0), // the first layer
file, // the absolute file path to the text file with absolute image file paths
" ", // the column separator <path> <x> <y> <section index>
1.0, // section thickness, defaults to 1
1.0, // calibration, defaults to 1
false, // whether to homogenize contrast, avoid
1.0f, // scaling factor, default to 1
0); // border width
/* wait until all images have been imported */
task.join();
// /* wait until all mipmaps are generated */
// futures = new ArrayList();
// for (p : layerset.getDisplayables(Patch.class))
// futures.add(p.updateMipMaps());
// for (f : futures)
// f.get();
/* delete reference layer if it was not part of the import range */
l0 = layerset.getLayer(0);
if (l0.getDisplayables(Patch.class).size() == 0)
layerset.remove(l0);
/* save the project */
project.saveAs(dir + "/project.xml", false);
/* prepare alignment */
layerRange = layerset.getLayers();
fixedLayers = new HashSet();
emptyLayers = new HashSet();
filter = new Filter() {
public boolean accept(Patch patch) {
return patch.isVisible();
}
};
/* affine alignment */
paramAffine = new RegularizedAffineLayerAlignment.Param();
paramAffine.ppm.sift.initialSigma = 1.6f;
paramAffine.ppm.sift.steps = 3;
paramAffine.ppm.sift.minOctaveSize = 200;
paramAffine.ppm.sift.maxOctaveSize = 1024;
paramAffine.ppm.sift.fdSize = 4;
paramAffine.ppm.sift.fdBins = 8;
paramAffine.ppm.rod = 0.92f;
paramAffine.ppm.clearCache = true;
paramAffine.ppm.maxNumThreadsSift = runtime.availableProcessors();
paramAffine.maxEpsilon = 50.0f;
paramAffine.minInlierRatio = 0.0f;
paramAffine.minNumInliers = 12;
paramAffine.expectedModelIndex = 0;
paramAffine.multipleHypotheses = true;
paramAffine.rejectIdentity = false;
paramAffine.identityTolerance = 0.0f;
paramAffine.maxNumNeighbors = 10;
paramAffine.maxNumFailures = 3;
paramAffine.maxNumThreads = runtime.availableProcessors();
paramAffine.desiredModelIndex = 3;
paramAffine.regularize = true;
paramAffine.maxIterationsOptimize = 2000;
paramAffine.maxPlateauwidthOptimize = 2000;
paramAffine.regularizerIndex = 0;
paramAffine.visualize = false;
paramAffine.lambda = 0.1f;
new RegularizedAffineLayerAlignment().exec(
paramAffine,
layerRange,
fixedLayers,
emptyLayers,
layerset.get2DBounds(),
false,
false,
filter);
paramAffine.lambda = 0.01f;
layerset.setMinimumDimensions();
new RegularizedAffineLayerAlignment().exec(
paramAffine,
layerRange,
fixedLayers,
emptyLayers,
layerset.get2DBounds(),
false,
false,
filter);
layerset.setMinimumDimensions();
/* save the project */
project.saveAs(dir + "/project.xml", true);
/* elastic alignment */
paramElastic = new ElasticLayerAlignment.Param();
paramElastic.layerScale = 0.25f;
paramElastic.searchRadius = 12;
paramElastic.blockRadius = 600;
paramElastic.resolutionSpringMesh = (int)Math.ceil((double)layerset.get2DBounds().width / 8192.0 * 32.0);
paramElastic.minR = 0.6f;
paramElastic.maxCurvatureR = 10.0f;
paramElastic.rodR = 0.92f;
paramElastic.useLocalSmoothnessFilter = true;
paramElastic.localModelIndex = 3;
paramElastic.localRegionSigma = 1000.0f;
paramElastic.maxLocalEpsilon = 1.0f;
paramElastic.maxLocalTrust = 4.0f;
paramElastic.isAligned = true;
paramElastic.maxNumFailures = 3;
paramElastic.maxNumNeighbors = 5;
paramElastic.maxNumThreads = 8;
paramElastic.desiredModelIndex = 1;
paramElastic.maxIterationsOptimize = 1000;
paramElastic.maxPlateauwidthOptimize = 200;
paramElastic.stiffnessSpringMesh = 0.25f;
paramElastic.maxStretchSpringMesh = 2000.0f;
paramElastic.maxIterationsSpringMesh = 5000;
paramElastic.maxPlateauwidthSpringMesh = 1000;
paramElastic.dampSpringMesh = 0.9f;
paramElastic.useLegacyOptimizer = false;
new ElasticLayerAlignment().exec(
paramElastic,
project,
layerRange,
fixedLayers,
emptyLayers,
layerset.get2DBounds(),
false,
false,
filter);
/* wait until all mipmaps are generated */
futures = new ArrayList();
for (p : layerset.getDisplayables(Patch.class))
futures.add(p.updateMipMaps());
for (f : futures)
f.get();
layerset.setMinimumDimensions();
/* save the project */
project.saveAs(dir + "/project.xml", true);
}
catch (e) {
e.printStackTrace();
}
/* shutdown */
runtime.exit(0);