diff --git a/changelog/9643.bugfix.rst b/changelog/9643.bugfix.rst
new file mode 100644
index 00000000000..24ca81182ed
--- /dev/null
+++ b/changelog/9643.bugfix.rst
@@ -0,0 +1,2 @@
+Delay issuing a :class:`~pytest.PytestWarning` about diamond inheritance involving :class:`~pytest.Item` and
+:class:`~pytest.Collector` so it can be filtered using :ref:`standard warning filters <warnings>`.
diff --git a/src/_pytest/nodes.py b/src/_pytest/nodes.py
index 6e8454ad7c6..e49c1b003e0 100644
--- a/src/_pytest/nodes.py
+++ b/src/_pytest/nodes.py
@@ -656,20 +656,6 @@ class Item(Node):
 
     nextitem = None
 
-    def __init_subclass__(cls) -> None:
-        problems = ", ".join(
-            base.__name__ for base in cls.__bases__ if issubclass(base, Collector)
-        )
-        if problems:
-            warnings.warn(
-                f"{cls.__name__} is an Item subclass and should not be a collector, "
-                f"however its bases {problems} are collectors.\n"
-                "Please split the Collectors and the Item into separate node types.\n"
-                "Pytest Doc example: https://docs.pytest.org/en/latest/example/nonpython.html\n"
-                "example pull request on a plugin: https://github.com/asmeurer/pytest-flakes/pull/40/",
-                PytestWarning,
-            )
-
     def __init__(
         self,
         name,
@@ -697,6 +683,37 @@ def __init__(
         #: for this test.
         self.user_properties: List[Tuple[str, object]] = []
 
+        self._check_item_and_collector_diamond_inheritance()
+
+    def _check_item_and_collector_diamond_inheritance(self) -> None:
+        """
+        Check if the current type inherits from both File and Collector
+        at the same time, emitting a warning accordingly (#8447).
+        """
+        cls = type(self)
+
+        # We inject an attribute in the type to avoid issuing this warning
+        # for the same class more than once, which is not helpful.
+        # It is a hack, but was deemed acceptable in order to avoid
+        # flooding the user in the common case.
+        attr_name = "_pytest_diamond_inheritance_warning_shown"
+        if getattr(cls, attr_name, False):
+            return
+        setattr(cls, attr_name, True)
+
+        problems = ", ".join(
+            base.__name__ for base in cls.__bases__ if issubclass(base, Collector)
+        )
+        if problems:
+            warnings.warn(
+                f"{cls.__name__} is an Item subclass and should not be a collector, "
+                f"however its bases {problems} are collectors.\n"
+                "Please split the Collectors and the Item into separate node types.\n"
+                "Pytest Doc example: https://docs.pytest.org/en/latest/example/nonpython.html\n"
+                "example pull request on a plugin: https://github.com/asmeurer/pytest-flakes/pull/40/",
+                PytestWarning,
+            )
+
     def runtest(self) -> None:
         """Run the test case for this item.
 
diff --git a/testing/test_nodes.py b/testing/test_nodes.py
index c8afe0252be..df1439e1c49 100644
--- a/testing/test_nodes.py
+++ b/testing/test_nodes.py
@@ -1,3 +1,5 @@
+import re
+import warnings
 from pathlib import Path
 from typing import cast
 from typing import List
@@ -58,30 +60,31 @@ def test_subclassing_both_item_and_collector_deprecated(
     request, tmp_path: Path
 ) -> None:
     """
-    Verifies we warn on diamond inheritance
-    as well as correctly managing legacy inheritance ctors with missing args
-    as found in plugins
+    Verifies we warn on diamond inheritance as well as correctly managing legacy
+    inheritance constructors with missing args as found in plugins.
     """
 
-    with pytest.warns(
-        PytestWarning,
-        match=(
-            "(?m)SoWrong is an Item subclass and should not be a collector, however its bases File are collectors.\n"
-            "Please split the Collectors and the Item into separate node types.\n.*"
-        ),
-    ):
+    # We do not expect any warnings messages to issued during class definition.
+    with warnings.catch_warnings():
+        warnings.simplefilter("error")
 
         class SoWrong(nodes.Item, nodes.File):
             def __init__(self, fspath, parent):
                 """Legacy ctor with legacy call # don't wana see"""
                 super().__init__(fspath, parent)
 
-    with pytest.warns(
-        PytestWarning, match=".*SoWrong.* not using a cooperative constructor.*"
-    ):
+    with pytest.warns(PytestWarning) as rec:
         SoWrong.from_parent(
             request.session, fspath=legacy_path(tmp_path / "broken.txt")
         )
+    messages = [str(x.message) for x in rec]
+    assert any(
+        re.search(".*SoWrong.* not using a cooperative constructor.*", x)
+        for x in messages
+    )
+    assert any(
+        re.search("(?m)SoWrong .* should not be a collector", x) for x in messages
+    )
 
 
 @pytest.mark.parametrize(